Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2449c

Experiment: 7H9 with copper (II) chloride 0.08 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2448c and Rv2449c are separated by 87 nucleotidesRv2449c and Rv2450c are separated by 89 nucleotidesRv2450c and Rv2451 are separated by 81 nucleotides Rv2448c: Rv2448c - Probable valyl-tRNA synthase protein ValS (valyl-tRNA synthetase) (valine--tRNA ligase) (valine translase), at 2,747,595 to 2,750,225 Rv2448c Rv2449c: Rv2449c - Conserved protein, at 2,750,313 to 2,751,572 Rv2449c Rv2450c: Rv2450c - Probable resuscitation-promoting factor RpfE, at 2,751,662 to 2,752,180 Rv2450c Rv2451: Rv2451 - Hypothetical proline and serine rich protein, at 2,752,262 to 2,752,660 Rv2451 Position (kb) 2750 2751 2752Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2750.266 kb on + strandat 2750.266 kb on + strandat 2750.267 kb on - strandat 2750.522 kb on - strand, within Rv2449cat 2750.548 kb on + strand, within Rv2449cat 2750.549 kb on - strand, within Rv2449cat 2750.549 kb on - strand, within Rv2449cat 2750.549 kb on - strand, within Rv2449cat 2750.549 kb on - strand, within Rv2449cat 2750.549 kb on - strand, within Rv2449cat 2750.564 kb on - strand, within Rv2449cat 2750.566 kb on + strand, within Rv2449cat 2750.626 kb on - strand, within Rv2449cat 2750.947 kb on + strand, within Rv2449cat 2750.947 kb on + strand, within Rv2449cat 2750.948 kb on - strand, within Rv2449cat 2750.948 kb on - strand, within Rv2449cat 2750.948 kb on - strand, within Rv2449cat 2750.958 kb on + strand, within Rv2449cat 2750.959 kb on - strand, within Rv2449cat 2750.985 kb on - strand, within Rv2449cat 2751.109 kb on + strand, within Rv2449cat 2751.112 kb on - strand, within Rv2449cat 2751.112 kb on - strand, within Rv2449cat 2751.118 kb on + strand, within Rv2449cat 2751.119 kb on - strand, within Rv2449cat 2751.119 kb on - strand, within Rv2449cat 2751.200 kb on - strand, within Rv2449cat 2751.250 kb on + strand, within Rv2449cat 2751.304 kb on + strand, within Rv2449cat 2751.304 kb on + strand, within Rv2449cat 2751.305 kb on - strand, within Rv2449cat 2751.496 kb on + strandat 2751.496 kb on + strandat 2751.497 kb on - strandat 2751.563 kb on + strandat 2751.584 kb on + strandat 2751.817 kb on - strand, within Rv2450cat 2751.817 kb on - strand, within Rv2450cat 2751.817 kb on - strand, within Rv2450cat 2751.817 kb on - strand, within Rv2450cat 2751.817 kb on - strand, within Rv2450cat 2751.819 kb on + strand, within Rv2450cat 2751.819 kb on + strand, within Rv2450cat 2751.820 kb on - strand, within Rv2450cat 2751.820 kb on - strand, within Rv2450cat 2751.891 kb on + strand, within Rv2450cat 2751.892 kb on - strand, within Rv2450cat 2751.892 kb on - strand, within Rv2450cat 2752.233 kb on + strandat 2752.390 kb on - strand, within Rv2451at 2752.447 kb on + strand, within Rv2451at 2752.479 kb on + strand, within Rv2451at 2752.483 kb on + strand, within Rv2451at 2752.563 kb on + strand, within Rv2451at 2752.563 kb on + strand, within Rv2451at 2752.563 kb on + strand, within Rv2451at 2752.564 kb on - strand, within Rv2451

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with copper (II) chloride 0.08 mM
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2,750,266 + -0.6
2,750,266 + +0.4
2,750,267 - -0.1
2,750,522 - Rv2449c 0.17 +0.3
2,750,548 + Rv2449c 0.19 +0.8
2,750,549 - Rv2449c 0.19 +0.9
2,750,549 - Rv2449c 0.19 +0.6
2,750,549 - Rv2449c 0.19 +0.4
2,750,549 - Rv2449c 0.19 +0.8
2,750,549 - Rv2449c 0.19 +0.5
2,750,564 - Rv2449c 0.20 +0.3
2,750,566 + Rv2449c 0.20 +0.2
2,750,626 - Rv2449c 0.25 -1.4
2,750,947 + Rv2449c 0.50 -0.1
2,750,947 + Rv2449c 0.50 +0.3
2,750,948 - Rv2449c 0.50 +1.9
2,750,948 - Rv2449c 0.50 +1.9
2,750,948 - Rv2449c 0.50 -1.1
2,750,958 + Rv2449c 0.51 +0.4
2,750,959 - Rv2449c 0.51 -0.3
2,750,985 - Rv2449c 0.53 -0.7
2,751,109 + Rv2449c 0.63 -1.5
2,751,112 - Rv2449c 0.63 +0.6
2,751,112 - Rv2449c 0.63 +1.0
2,751,118 + Rv2449c 0.64 -1.1
2,751,119 - Rv2449c 0.64 -0.0
2,751,119 - Rv2449c 0.64 +0.5
2,751,200 - Rv2449c 0.70 -0.3
2,751,250 + Rv2449c 0.74 +0.0
2,751,304 + Rv2449c 0.79 -0.1
2,751,304 + Rv2449c 0.79 -1.8
2,751,305 - Rv2449c 0.79 +0.4
2,751,496 + -0.8
2,751,496 + -0.1
2,751,497 - +0.1
2,751,563 + -1.7
2,751,584 + +0.3
2,751,817 - Rv2450c 0.30 +1.2
2,751,817 - Rv2450c 0.30 +0.2
2,751,817 - Rv2450c 0.30 +2.1
2,751,817 - Rv2450c 0.30 +0.3
2,751,817 - Rv2450c 0.30 -1.4
2,751,819 + Rv2450c 0.30 -0.3
2,751,819 + Rv2450c 0.30 -1.4
2,751,820 - Rv2450c 0.30 +0.4
2,751,820 - Rv2450c 0.30 +1.9
2,751,891 + Rv2450c 0.44 -0.7
2,751,892 - Rv2450c 0.44 -0.6
2,751,892 - Rv2450c 0.44 +1.0
2,752,233 + +0.3
2,752,390 - Rv2451 0.32 +0.2
2,752,447 + Rv2451 0.46 -4.1
2,752,479 + Rv2451 0.54 +1.0
2,752,483 + Rv2451 0.55 -0.5
2,752,563 + Rv2451 0.75 -0.2
2,752,563 + Rv2451 0.75 +0.1
2,752,563 + Rv2451 0.75 -0.5
2,752,564 - Rv2451 0.76 +0.3

Or see this region's nucleotide sequence