Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1485

Experiment: 7H9 with Norfloxacin 0.125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1483 and Rv1484 are separated by 18 nucleotidesRv1484 and Rv1485 are separated by 5 nucleotidesRv1485 and Rv1486c overlap by 35 nucleotidesRv1486c and Rv1487 are separated by 57 nucleotides Rv1483: Rv1483 - 3-oxoacyl-[acyl-carrier protein] reductase FabG1 (3-ketoacyl-acyl carrier protein reductase) (mycolic acid biosynthesis a protein), at 1,673,440 to 1,674,183 Rv1483 Rv1484: Rv1484 - NADH-dependent enoyl-[acyl-carrier-protein] reductase InhA (NADH-dependent enoyl-ACP reductase), at 1,674,202 to 1,675,011 Rv1484 Rv1485: Rv1485 - Ferrochelatase HemZ (protoheme ferro-lyase) (heme synthetase), at 1,675,017 to 1,676,051 Rv1485 Rv1486c: Rv1486c - Conserved hypothetical protein, at 1,676,017 to 1,676,883 Rv1486c Rv1487: Rv1487 - Conserved membrane protein, at 1,676,941 to 1,677,375 Rv1487 Position (kb) 1675 1676 1677Strain fitness (log2 ratio) -2 -1 0 1 2at 1676.304 kb on + strand, within Rv1486cat 1676.304 kb on + strand, within Rv1486cat 1676.305 kb on - strand, within Rv1486cat 1676.305 kb on - strand, within Rv1486cat 1676.387 kb on + strand, within Rv1486cat 1676.387 kb on + strand, within Rv1486cat 1676.388 kb on - strand, within Rv1486cat 1676.420 kb on + strand, within Rv1486cat 1676.421 kb on - strand, within Rv1486cat 1676.421 kb on - strand, within Rv1486cat 1676.762 kb on + strand, within Rv1486cat 1676.896 kb on + strandat 1676.896 kb on + strandat 1676.930 kb on - strandat 1676.935 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Norfloxacin 0.125 mM
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1,676,304 + Rv1486c 0.33 -0.2
1,676,304 + Rv1486c 0.33 -0.6
1,676,305 - Rv1486c 0.33 +0.1
1,676,305 - Rv1486c 0.33 +0.1
1,676,387 + Rv1486c 0.43 +0.1
1,676,387 + Rv1486c 0.43 +0.1
1,676,388 - Rv1486c 0.43 +2.2
1,676,420 + Rv1486c 0.46 -0.5
1,676,421 - Rv1486c 0.47 -0.1
1,676,421 - Rv1486c 0.47 -0.5
1,676,762 + Rv1486c 0.86 +0.3
1,676,896 + -2.0
1,676,896 + -0.6
1,676,930 - -0.4
1,676,935 - -0.8

Or see this region's nucleotide sequence