Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0001

Experiment: 7H9 with methylglyoxal 1.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0001 and Rv0002 are separated by 527 nucleotides Rv0001: Rv0001 - Chromosomal replication initiator protein DnaA, at 1 to 1,524 Rv0001 Rv0002: Rv0002 - DNA polymerase III (beta chain) DnaN (DNA nucleotidyltransferase), at 2,052 to 3,260 Rv0002 Position (kb) 0 1 2Strain fitness (log2 ratio) -1 0 1at 1.635 kb on + strandat 1.635 kb on + strandat 1.635 kb on + strandat 1.636 kb on - strandat 1.858 kb on + strandat 1.858 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with methylglyoxal 1.2 mM
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1,635 + -1.0
1,635 + -1.0
1,635 + +0.3
1,636 - +0.8
1,858 + -1.4
1,858 + -0.9

Or see this region's nucleotide sequence