Experiment: 7H9 with methylglyoxal 1.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv1413 and Rv1414 overlap by 11 nucleotides Rv1414 and Rv1415 are separated by 104 nucleotides Rv1415 and Rv1416 overlap by 4 nucleotides Rv1416 and Rv1417 overlap by 4 nucleotides Rv1417 and Rv1418 are separated by 24 nucleotides
Rv1413: Rv1413 - Conserved hypothetical protein, at 1,589,386 to 1,589,901
Rv1413
Rv1414: Rv1414 - Conserved hypothetical protein, at 1,589,891 to 1,590,292
Rv1414
Rv1415: Rv1415 - Probable riboflavin biosynthesis protein RibA2 : GTP cyclohydrolase II + 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase), at 1,590,397 to 1,591,674
Rv1415
Rv1416: Rv1416 - Probable riboflavin synthase beta chain RibH (6,7-dimethyl-8-ribityllumazine synthase) (DMRL synthase) (lumazine synthase), at 1,591,671 to 1,592,153
Rv1416
Rv1417: Rv1417 - Possible conserved membrane protein, at 1,592,150 to 1,592,614
Rv1417
Rv1418: Rv1418 - Probable lipoprotein LprH, at 1,592,639 to 1,593,325
Rv1418
Position (kb)
1590
1591
1592 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1589.411 kb on + strand at 1589.411 kb on + strand at 1589.412 kb on - strand at 1589.438 kb on + strand, within Rv1413 at 1589.439 kb on - strand, within Rv1413 at 1589.439 kb on - strand, within Rv1413 at 1589.439 kb on - strand, within Rv1413 at 1589.439 kb on - strand, within Rv1413 at 1589.439 kb on - strand, within Rv1413 at 1589.449 kb on + strand, within Rv1413 at 1589.449 kb on + strand, within Rv1413 at 1589.450 kb on - strand, within Rv1413 at 1589.450 kb on - strand, within Rv1413 at 1589.888 kb on + strand at 1589.888 kb on + strand at 1589.889 kb on - strand at 1589.889 kb on - strand at 1590.216 kb on + strand, within Rv1414 at 1590.317 kb on + strand at 1590.339 kb on + strand at 1592.505 kb on - strand, within Rv1417 at 1592.628 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with methylglyoxal 1.2 mM remove 1,589,411 + -0.1 1,589,411 + +0.4 1,589,412 - +0.8 1,589,438 + Rv1413 0.10 +0.0 1,589,439 - Rv1413 0.10 -0.5 1,589,439 - Rv1413 0.10 +0.3 1,589,439 - Rv1413 0.10 -1.3 1,589,439 - Rv1413 0.10 -1.1 1,589,439 - Rv1413 0.10 +1.3 1,589,449 + Rv1413 0.12 -0.4 1,589,449 + Rv1413 0.12 -0.4 1,589,450 - Rv1413 0.12 +0.0 1,589,450 - Rv1413 0.12 -0.3 1,589,888 + -1.0 1,589,888 + -0.3 1,589,889 - +2.8 1,589,889 - -0.4 1,590,216 + Rv1414 0.81 -1.3 1,590,317 + -0.4 1,590,339 + -0.0 1,592,505 - Rv1417 0.76 -1.7 1,592,628 - -1.5
Or see this region's nucleotide sequence