Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1415

Experiment: 7H9 with methylglyoxal 1.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1413 and Rv1414 overlap by 11 nucleotidesRv1414 and Rv1415 are separated by 104 nucleotidesRv1415 and Rv1416 overlap by 4 nucleotidesRv1416 and Rv1417 overlap by 4 nucleotidesRv1417 and Rv1418 are separated by 24 nucleotides Rv1413: Rv1413 - Conserved hypothetical protein, at 1,589,386 to 1,589,901 Rv1413 Rv1414: Rv1414 - Conserved hypothetical protein, at 1,589,891 to 1,590,292 Rv1414 Rv1415: Rv1415 - Probable riboflavin biosynthesis protein RibA2 : GTP cyclohydrolase II + 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase), at 1,590,397 to 1,591,674 Rv1415 Rv1416: Rv1416 - Probable riboflavin synthase beta chain RibH (6,7-dimethyl-8-ribityllumazine synthase) (DMRL synthase) (lumazine synthase), at 1,591,671 to 1,592,153 Rv1416 Rv1417: Rv1417 - Possible conserved membrane protein, at 1,592,150 to 1,592,614 Rv1417 Rv1418: Rv1418 - Probable lipoprotein LprH, at 1,592,639 to 1,593,325 Rv1418 Position (kb) 1590 1591 1592Strain fitness (log2 ratio) -1 0 1 2 3at 1589.411 kb on + strandat 1589.411 kb on + strandat 1589.412 kb on - strandat 1589.438 kb on + strand, within Rv1413at 1589.439 kb on - strand, within Rv1413at 1589.439 kb on - strand, within Rv1413at 1589.439 kb on - strand, within Rv1413at 1589.439 kb on - strand, within Rv1413at 1589.439 kb on - strand, within Rv1413at 1589.449 kb on + strand, within Rv1413at 1589.449 kb on + strand, within Rv1413at 1589.450 kb on - strand, within Rv1413at 1589.450 kb on - strand, within Rv1413at 1589.888 kb on + strandat 1589.888 kb on + strandat 1589.889 kb on - strandat 1589.889 kb on - strandat 1590.216 kb on + strand, within Rv1414at 1590.317 kb on + strandat 1590.339 kb on + strandat 1592.505 kb on - strand, within Rv1417at 1592.628 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with methylglyoxal 1.2 mM
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1,589,411 + -0.1
1,589,411 + +0.4
1,589,412 - +0.8
1,589,438 + Rv1413 0.10 +0.0
1,589,439 - Rv1413 0.10 -0.5
1,589,439 - Rv1413 0.10 +0.3
1,589,439 - Rv1413 0.10 -1.3
1,589,439 - Rv1413 0.10 -1.1
1,589,439 - Rv1413 0.10 +1.3
1,589,449 + Rv1413 0.12 -0.4
1,589,449 + Rv1413 0.12 -0.4
1,589,450 - Rv1413 0.12 +0.0
1,589,450 - Rv1413 0.12 -0.3
1,589,888 + -1.0
1,589,888 + -0.3
1,589,889 - +2.8
1,589,889 - -0.4
1,590,216 + Rv1414 0.81 -1.3
1,590,317 + -0.4
1,590,339 + -0.0
1,592,505 - Rv1417 0.76 -1.7
1,592,628 - -1.5

Or see this region's nucleotide sequence