Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0387c

Experiment: 7H9 with Ramoplanin 0.009 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0386 and Rv0387c are separated by 3 nucleotidesRv0387c and Rv0388c are separated by 52 nucleotidesRv0388c and Rv0389 are separated by 333 nucleotides Rv0386: Rv0386 - Probable transcriptional regulatory protein (probably LuxR/UhpA-family), at 463,411 to 466,668 Rv0386 Rv0387c: Rv0387c - Conserved hypothetical protein, at 466,672 to 467,406 Rv0387c Rv0388c: Rv0388c - PPE family protein PPE9, at 467,459 to 468,001 Rv0388c Rv0389: Rv0389 - Probable phosphoribosylglycinamide formyltransferase 2 PurT (GART 2) (gar transformylase 2) (5'-phosphoribosylglycinamide transformylase 2) (formate-dependent gar transformylase), at 468,335 to 469,594 Rv0389 Position (kb) 466 467 468Strain fitness (log2 ratio) -1 0 1 2at 465.775 kb on + strand, within Rv0386at 465.798 kb on + strand, within Rv0386at 465.845 kb on - strand, within Rv0386at 465.863 kb on - strand, within Rv0386at 466.020 kb on + strand, within Rv0386at 466.152 kb on + strand, within Rv0386at 466.285 kb on - strand, within Rv0386at 466.321 kb on + strand, within Rv0386at 466.321 kb on + strand, within Rv0386at 466.606 kb on + strandat 466.663 kb on - strandat 466.674 kb on - strandat 466.789 kb on + strand, within Rv0387cat 466.789 kb on + strand, within Rv0387cat 466.790 kb on - strand, within Rv0387cat 466.790 kb on - strand, within Rv0387cat 466.973 kb on + strand, within Rv0387cat 466.998 kb on + strand, within Rv0387cat 466.998 kb on + strand, within Rv0387cat 466.998 kb on + strand, within Rv0387cat 466.999 kb on - strand, within Rv0387cat 467.000 kb on + strand, within Rv0387cat 467.001 kb on - strand, within Rv0387cat 467.001 kb on - strand, within Rv0387cat 467.001 kb on - strand, within Rv0387cat 467.001 kb on - strand, within Rv0387cat 467.001 kb on - strand, within Rv0387cat 467.396 kb on - strandat 467.396 kb on - strandat 467.396 kb on - strandat 467.431 kb on + strandat 467.432 kb on - strandat 467.596 kb on + strand, within Rv0388cat 467.701 kb on - strand, within Rv0388cat 467.787 kb on + strand, within Rv0388cat 467.787 kb on + strand, within Rv0388cat 467.788 kb on - strand, within Rv0388cat 467.868 kb on + strand, within Rv0388cat 467.955 kb on + strandat 467.955 kb on + strandat 467.955 kb on + strandat 468.139 kb on + strandat 468.140 kb on - strandat 468.140 kb on - strandat 468.140 kb on - strandat 468.140 kb on - strandat 468.140 kb on - strandat 468.140 kb on - strandat 468.140 kb on - strandat 468.143 kb on + strandat 468.143 kb on + strandat 468.185 kb on - strandat 468.247 kb on + strandat 468.296 kb on + strandat 468.296 kb on + strandat 468.296 kb on + strandat 468.296 kb on + strandat 468.297 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ramoplanin 0.009 mM
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465,775 + Rv0386 0.73 -0.5
465,798 + Rv0386 0.73 -0.4
465,845 - Rv0386 0.75 -0.5
465,863 - Rv0386 0.75 -0.7
466,020 + Rv0386 0.80 -0.2
466,152 + Rv0386 0.84 -0.0
466,285 - Rv0386 0.88 +1.4
466,321 + Rv0386 0.89 -0.4
466,321 + Rv0386 0.89 +0.0
466,606 + -0.6
466,663 - +0.5
466,674 - +0.3
466,789 + Rv0387c 0.16 -0.0
466,789 + Rv0387c 0.16 -0.5
466,790 - Rv0387c 0.16 -0.8
466,790 - Rv0387c 0.16 -0.4
466,973 + Rv0387c 0.41 -0.6
466,998 + Rv0387c 0.44 -0.6
466,998 + Rv0387c 0.44 -0.5
466,998 + Rv0387c 0.44 -0.7
466,999 - Rv0387c 0.44 +1.9
467,000 + Rv0387c 0.45 +0.9
467,001 - Rv0387c 0.45 -0.7
467,001 - Rv0387c 0.45 -0.9
467,001 - Rv0387c 0.45 +1.9
467,001 - Rv0387c 0.45 -0.3
467,001 - Rv0387c 0.45 -0.1
467,396 - +0.3
467,396 - -0.7
467,396 - -0.8
467,431 + -0.5
467,432 - +0.2
467,596 + Rv0388c 0.25 -1.3
467,701 - Rv0388c 0.45 +0.9
467,787 + Rv0388c 0.60 +0.3
467,787 + Rv0388c 0.60 -1.0
467,788 - Rv0388c 0.61 -0.5
467,868 + Rv0388c 0.75 -0.0
467,955 + -0.4
467,955 + +0.0
467,955 + +0.0
468,139 + -1.5
468,140 - +1.9
468,140 - -0.0
468,140 - +0.1
468,140 - +0.3
468,140 - -0.3
468,140 - +0.7
468,140 - -0.2
468,143 + +0.5
468,143 + +0.1
468,185 - -0.3
468,247 + -0.3
468,296 + +1.9
468,296 + -0.7
468,296 + -0.7
468,296 + -1.7
468,297 - -0.2

Or see this region's nucleotide sequence