Experiment: 7H9 with Ciprofloxacin Hydrochloride 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3127 and Rv3128c overlap by 14 nucleotides Rv3128c and Rv3129 are separated by 478 nucleotides Rv3129 and Rv3130c overlap by 18 nucleotides
Rv3127: Rv3127 - Conserved protein, at 3,492,147 to 3,493,181
Rv3127
Rv3128c: Rv3128c - Conserved hypothetical protein, at 3,493,168 to 3,494,181
Rv3128c
Rv3129: Rv3129 - Conserved hypothetical protein, at 3,494,660 to 3,494,992
Rv3129
Rv3130c: Rv3130c - Triacylglycerol synthase (diacylglycerol acyltransferase) Tgs1, at 3,494,975 to 3,496,366
Rv3130c
Position (kb)
3493
3494
3495 Strain fitness (log2 ratio)
-2
-1
0
1 at 3492.525 kb on + strand, within Rv3127 at 3492.793 kb on - strand, within Rv3127 at 3492.815 kb on - strand, within Rv3127 at 3492.907 kb on + strand, within Rv3127 at 3493.130 kb on + strand at 3493.143 kb on - strand at 3493.232 kb on + strand at 3493.233 kb on - strand at 3493.328 kb on + strand, within Rv3128c at 3493.559 kb on + strand, within Rv3128c at 3493.637 kb on + strand, within Rv3128c at 3493.638 kb on - strand, within Rv3128c at 3493.642 kb on - strand, within Rv3128c at 3494.213 kb on + strand at 3494.214 kb on - strand at 3494.382 kb on - strand at 3494.476 kb on + strand at 3494.476 kb on + strand at 3494.477 kb on - strand at 3495.121 kb on - strand, within Rv3130c at 3495.136 kb on - strand, within Rv3130c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Ciprofloxacin Hydrochloride 0.001 mM remove 3,492,525 + Rv3127 0.37 -0.5 3,492,793 - Rv3127 0.62 -0.5 3,492,815 - Rv3127 0.65 -0.4 3,492,907 + Rv3127 0.73 +0.3 3,493,130 + -0.8 3,493,143 - -0.4 3,493,232 + -1.0 3,493,233 - +1.3 3,493,328 + Rv3128c 0.16 +0.2 3,493,559 + Rv3128c 0.39 +0.4 3,493,637 + Rv3128c 0.46 +1.2 3,493,638 - Rv3128c 0.46 -0.4 3,493,642 - Rv3128c 0.47 +0.3 3,494,213 + -0.0 3,494,214 - -1.8 3,494,382 - +0.7 3,494,476 + +0.0 3,494,476 + +0.7 3,494,477 - +0.1 3,495,121 - Rv3130c 0.10 -0.6 3,495,136 - Rv3130c 0.12 +0.1
Or see this region's nucleotide sequence