Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1614

Experiment: 7H9 with Ciprofloxacin Hydrochloride 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1612 and Rv1613 overlap by 1 nucleotidesRv1613 and Rv1614 overlap by 1 nucleotidesRv1614 and Rv1615 are separated by 675 nucleotides Rv1612: Rv1612 - Tryptophan synthase, beta subunit TrpB, at 1,811,127 to 1,812,359 Rv1612 Rv1613: Rv1613 - Probable tryptophan synthase, alpha subunit TrpA, at 1,812,359 to 1,813,171 Rv1613 Rv1614: Rv1614 - Possible prolipoprotein diacylglyceryl transferases Lgt, at 1,813,171 to 1,814,577 Rv1614 Rv1615: Rv1615 - Probable membrane protein, at 1,815,253 to 1,815,693 Rv1615 Position (kb) 1813 1814 1815Strain fitness (log2 ratio) -2 -1 0 1at 1814.038 kb on + strand, within Rv1614at 1814.038 kb on + strand, within Rv1614at 1814.620 kb on + strandat 1814.621 kb on - strandat 1814.621 kb on - strandat 1814.707 kb on + strandat 1814.747 kb on - strandat 1814.756 kb on - strandat 1814.761 kb on - strandat 1814.850 kb on + strandat 1814.850 kb on + strandat 1814.850 kb on + strandat 1814.850 kb on + strandat 1814.851 kb on - strandat 1814.851 kb on - strandat 1814.861 kb on - strandat 1814.925 kb on - strandat 1815.409 kb on + strand, within Rv1615at 1815.409 kb on + strand, within Rv1615at 1815.409 kb on + strand, within Rv1615at 1815.409 kb on + strand, within Rv1615at 1815.539 kb on - strand, within Rv1615at 1815.549 kb on + strand, within Rv1615

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ciprofloxacin Hydrochloride 0.001 mM
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1,814,038 + Rv1614 0.62 +0.4
1,814,038 + Rv1614 0.62 +0.4
1,814,620 + +0.6
1,814,621 - -0.5
1,814,621 - -0.1
1,814,707 + -0.3
1,814,747 - -0.2
1,814,756 - -2.0
1,814,761 - +0.0
1,814,850 + +0.2
1,814,850 + -0.2
1,814,850 + +0.1
1,814,850 + -0.3
1,814,851 - -0.3
1,814,851 - -0.5
1,814,861 - +0.6
1,814,925 - -0.3
1,815,409 + Rv1615 0.35 +0.5
1,815,409 + Rv1615 0.35 +0.3
1,815,409 + Rv1615 0.35 +0.3
1,815,409 + Rv1615 0.35 +0.6
1,815,539 - Rv1615 0.65 -0.3
1,815,549 + Rv1615 0.67 -0.5

Or see this region's nucleotide sequence