Experiment: 7H9 with Ciprofloxacin Hydrochloride 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0777 and Rv0778 are separated by 4 nucleotides Rv0778 and Rv0779c overlap by 4 nucleotides Rv0779c and Rv0780 are separated by 50 nucleotides
Rv0777: Rv0777 - Probable adenylosuccinate lyase PurB (adenylosuccinase) (ASL) (ASASE), at 870,008 to 871,426
Rv0777
Rv0778: Rv0778 - Possible cytochrome P450 126 Cyp126, at 871,431 to 872,675
Rv0778
Rv0779c: Rv0779c - Possible conserved transmembrane protein, at 872,672 to 873,292
Rv0779c
Rv0780: Rv0780 - Phosphoribosylaminoimidazole-succinocarboxamide synthase PurC (SAICAR synthetase), at 873,343 to 874,236
Rv0780
Position (kb) 871
872
873 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 871.436 kb on + strand at 871.540 kb on - strand at 871.638 kb on + strand, within Rv0778 at 871.639 kb on - strand, within Rv0778 at 871.840 kb on + strand, within Rv0778 at 871.840 kb on + strand, within Rv0778 at 872.037 kb on + strand, within Rv0778 at 872.038 kb on - strand, within Rv0778 at 872.043 kb on + strand, within Rv0778 at 872.043 kb on + strand, within Rv0778 at 872.044 kb on - strand, within Rv0778 at 872.067 kb on - strand, within Rv0778 at 872.160 kb on + strand, within Rv0778 at 872.160 kb on + strand, within Rv0778 at 872.161 kb on - strand, within Rv0778 at 872.621 kb on + strand at 872.674 kb on - strand at 873.301 kb on + strand at 873.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Ciprofloxacin Hydrochloride 0.001 mM remove 871,436 + +0.0 871,540 - -0.5 871,638 + Rv0778 0.17 +0.0 871,639 - Rv0778 0.17 -0.6 871,840 + Rv0778 0.33 -0.6 871,840 + Rv0778 0.33 -1.1 872,037 + Rv0778 0.49 +2.9 872,038 - Rv0778 0.49 -0.3 872,043 + Rv0778 0.49 -1.0 872,043 + Rv0778 0.49 -0.6 872,044 - Rv0778 0.49 -1.2 872,067 - Rv0778 0.51 -0.0 872,160 + Rv0778 0.59 -3.2 872,160 + Rv0778 0.59 -0.3 872,161 - Rv0778 0.59 -0.2 872,621 + -1.4 872,674 - +0.4 873,301 + -0.6 873,302 - -0.2
Or see this region's nucleotide sequence