Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0621

Experiment: 7H9 with Ciprofloxacin Hydrochloride 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0620 and Rv0621 are separated by 394 nucleotidesRv0621 and Rv0622 are separated by 103 nucleotides Rv0620: Rv0620 - Probable galactokinase GalK (galactose kinase), at 712,716 to 713,807 Rv0620 Rv0621: Rv0621 - Possible membrane protein, at 714,202 to 715,266 Rv0621 Rv0622: Rv0622 - Possible membrane protein, at 715,370 to 716,317 Rv0622 Position (kb) 714 715 716Strain fitness (log2 ratio) -1 0 1 2at 714.061 kb on - strandat 714.290 kb on + strandat 714.290 kb on + strandat 714.291 kb on - strandat 714.291 kb on - strandat 714.322 kb on + strand, within Rv0621at 715.437 kb on - strandat 715.542 kb on - strand, within Rv0622at 715.542 kb on - strand, within Rv0622at 715.796 kb on + strand, within Rv0622at 715.797 kb on - strand, within Rv0622at 716.231 kb on + strandat 716.232 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ciprofloxacin Hydrochloride 0.001 mM
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714,061 - -0.2
714,290 + -0.4
714,290 + -1.0
714,291 - -1.5
714,291 - -0.2
714,322 + Rv0621 0.11 -0.7
715,437 - +1.1
715,542 - Rv0622 0.18 -0.5
715,542 - Rv0622 0.18 +2.2
715,796 + Rv0622 0.45 -0.1
715,797 - Rv0622 0.45 -1.7
716,231 + +0.0
716,232 - -0.2

Or see this region's nucleotide sequence