Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0216

Experiment: 7H9 with Ciprofloxacin Hydrochloride 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0215c and Rv0216 are separated by 56 nucleotidesRv0216 and Rv0217c overlap by 4 nucleotidesRv0217c and Rv0218 are separated by 92 nucleotides Rv0215c: Rv0215c - Probable acyl-CoA dehydrogenase FadE3, at 257,783 to 258,856 Rv0215c Rv0216: Rv0216 - Double hotdog hydratase, at 258,913 to 259,926 Rv0216 Rv0217c: Rv0217c - Possible esterase LipW, at 259,923 to 260,831 Rv0217c Rv0218: Rv0218 - Probable conserved transmembrane protein, at 260,924 to 262,252 Rv0218 Position (kb) 258 259 260Strain fitness (log2 ratio) -1 0 1 2at 257.926 kb on - strand, within Rv0215cat 257.991 kb on + strand, within Rv0215cat 257.992 kb on - strand, within Rv0215cat 258.531 kb on + strand, within Rv0215cat 258.532 kb on - strand, within Rv0215cat 258.532 kb on - strand, within Rv0215cat 258.532 kb on - strand, within Rv0215cat 258.651 kb on + strand, within Rv0215cat 258.681 kb on + strand, within Rv0215cat 258.747 kb on + strand, within Rv0215cat 258.751 kb on + strandat 258.751 kb on + strandat 258.751 kb on + strandat 258.752 kb on - strandat 258.752 kb on - strandat 259.193 kb on - strand, within Rv0216at 259.924 kb on + strandat 259.935 kb on - strandat 259.974 kb on - strandat 260.050 kb on - strand, within Rv0217cat 260.157 kb on + strand, within Rv0217cat 260.209 kb on + strand, within Rv0217cat 260.209 kb on + strand, within Rv0217cat 260.209 kb on + strand, within Rv0217cat 260.210 kb on - strand, within Rv0217cat 260.255 kb on - strand, within Rv0217cat 260.302 kb on + strand, within Rv0217cat 260.455 kb on + strand, within Rv0217cat 260.584 kb on - strand, within Rv0217cat 260.702 kb on + strand, within Rv0217c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ciprofloxacin Hydrochloride 0.001 mM
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257,926 - Rv0215c 0.13 -0.5
257,991 + Rv0215c 0.19 -0.3
257,992 - Rv0215c 0.19 -0.7
258,531 + Rv0215c 0.70 -1.0
258,532 - Rv0215c 0.70 +0.9
258,532 - Rv0215c 0.70 +1.2
258,532 - Rv0215c 0.70 +0.3
258,651 + Rv0215c 0.81 -0.4
258,681 + Rv0215c 0.84 -0.5
258,747 + Rv0215c 0.90 +0.7
258,751 + -0.9
258,751 + +0.5
258,751 + -0.1
258,752 - +2.2
258,752 - -0.5
259,193 - Rv0216 0.28 +0.3
259,924 + +0.8
259,935 - -0.8
259,974 - +1.3
260,050 - Rv0217c 0.14 -0.0
260,157 + Rv0217c 0.26 -1.1
260,209 + Rv0217c 0.31 -0.7
260,209 + Rv0217c 0.31 +0.0
260,209 + Rv0217c 0.31 -0.5
260,210 - Rv0217c 0.32 +0.5
260,255 - Rv0217c 0.37 -0.4
260,302 + Rv0217c 0.42 -0.1
260,455 + Rv0217c 0.59 -1.1
260,584 - Rv0217c 0.73 -0.5
260,702 + Rv0217c 0.86 +1.3

Or see this region's nucleotide sequence