Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2002

Experiment: 7H9 with Irgasan 0.065 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntRv2000 and Rv2001 are separated by 9 nucleotidesRv2001 and Rv2002 are separated by 75 nucleotidesRv2002 and Rv2003c are separated by 120 nucleotides Rv2000: Rv2000 - Unknown protein, at 2,245,209 to 2,246,822 Rv2000 Rv2001: Rv2001 - Conserved hypothetical protein, at 2,246,832 to 2,247,584 Rv2001 Rv2002: Rv2002 - Possible 20-beta-hydroxysteroid dehydrogenase FabG3 (cortisone reductase) ((R)-20-hydroxysteroid dehydrogenase), at 2,247,660 to 2,248,442 Rv2002 Rv2003c: Rv2003c - Conserved hypothetical protein, at 2,248,563 to 2,249,420 Rv2003c Position (kb) 2247 2248 2249Strain fitness (log2 ratio) -2 -1 0 1at 2246.776 kb on + strandat 2246.893 kb on - strandat 2246.893 kb on - strandat 2247.409 kb on + strand, within Rv2001at 2247.410 kb on - strand, within Rv2001at 2247.580 kb on + strandat 2247.580 kb on + strandat 2247.713 kb on + strandat 2248.376 kb on - strandat 2248.440 kb on + strandat 2248.565 kb on - strandat 2248.593 kb on - strandat 2248.771 kb on + strand, within Rv2003cat 2248.823 kb on - strand, within Rv2003cat 2249.326 kb on - strand, within Rv2003c

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Irgasan 0.065 mM
remove
2,246,776 + -0.4
2,246,893 - +1.0
2,246,893 - +0.2
2,247,409 + Rv2001 0.77 +0.0
2,247,410 - Rv2001 0.77 -0.6
2,247,580 + +0.2
2,247,580 + -1.0
2,247,713 + -0.6
2,248,376 - -0.4
2,248,440 + -1.3
2,248,565 - -0.5
2,248,593 - -0.5
2,248,771 + Rv2003c 0.24 -2.1
2,248,823 - Rv2003c 0.30 -1.4
2,249,326 - Rv2003c 0.89 +0.9

Or see this region's nucleotide sequence