Experiment: 7H9 with Linezolid 0.44 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv1415 and Rv1416 overlap by 4 nucleotides Rv1416 and Rv1417 overlap by 4 nucleotides Rv1417 and Rv1418 are separated by 24 nucleotides Rv1418 and Rv1419 are separated by 179 nucleotides Rv1419 and Rv1420 are separated by 63 nucleotides
Rv1415: Rv1415 - Probable riboflavin biosynthesis protein RibA2 : GTP cyclohydrolase II + 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase), at 1,590,397 to 1,591,674
Rv1415
Rv1416: Rv1416 - Probable riboflavin synthase beta chain RibH (6,7-dimethyl-8-ribityllumazine synthase) (DMRL synthase) (lumazine synthase), at 1,591,671 to 1,592,153
Rv1416
Rv1417: Rv1417 - Possible conserved membrane protein, at 1,592,150 to 1,592,614
Rv1417
Rv1418: Rv1418 - Probable lipoprotein LprH, at 1,592,639 to 1,593,325
Rv1418
Rv1419: Rv1419 - Unknown protein, at 1,593,505 to 1,593,978
Rv1419
Rv1420: Rv1420 - Probable excinuclease ABC (subunit C-nuclease) UvrC, at 1,594,042 to 1,595,982
Rv1420
Position (kb)
1592
1593
1594 Strain fitness (log2 ratio)
-1
0
1 at 1592.505 kb on - strand, within Rv1417 at 1592.628 kb on - strand at 1592.876 kb on + strand, within Rv1418 at 1592.876 kb on + strand, within Rv1418 at 1592.975 kb on + strand, within Rv1418 at 1592.976 kb on - strand, within Rv1418 at 1592.978 kb on - strand, within Rv1418 at 1592.978 kb on - strand, within Rv1418 at 1593.171 kb on + strand, within Rv1418 at 1593.171 kb on + strand, within Rv1418 at 1593.172 kb on - strand, within Rv1418 at 1593.515 kb on + strand at 1593.515 kb on + strand at 1593.516 kb on - strand at 1594.031 kb on - strand at 1594.035 kb on + strand at 1594.036 kb on - strand at 1594.036 kb on - strand at 1594.036 kb on - strand at 1594.039 kb on + strand at 1594.039 kb on + strand at 1594.039 kb on + strand at 1594.135 kb on + strand at 1594.178 kb on - strand at 1594.288 kb on + strand, within Rv1420
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Linezolid 0.44 ug/mL remove 1,592,505 - Rv1417 0.76 +0.3 1,592,628 - +0.5 1,592,876 + Rv1418 0.34 -0.3 1,592,876 + Rv1418 0.34 -0.6 1,592,975 + Rv1418 0.49 -0.6 1,592,976 - Rv1418 0.49 -1.5 1,592,978 - Rv1418 0.49 +0.3 1,592,978 - Rv1418 0.49 -0.4 1,593,171 + Rv1418 0.77 +1.2 1,593,171 + Rv1418 0.77 -1.3 1,593,172 - Rv1418 0.78 -0.6 1,593,515 + -0.6 1,593,515 + -0.5 1,593,516 - +1.0 1,594,031 - -0.1 1,594,035 + +0.6 1,594,036 - -0.4 1,594,036 - -1.2 1,594,036 - +0.8 1,594,039 + -0.1 1,594,039 + -1.7 1,594,039 + +1.4 1,594,135 + -0.5 1,594,178 - -0.9 1,594,288 + Rv1420 0.13 +0.2
Or see this region's nucleotide sequence