Experiment: 7H9 with Linezolid 0.44 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0442c and Rv0443 are separated by 181 nucleotides Rv0443 and Rv0444c are separated by 179 nucleotides Rv0444c and Rv0445c are separated by 43 nucleotides Rv0445c and Rv0446c are separated by 48 nucleotides
Rv0442c: Rv0442c - PPE family protein PPE10, at 530,751 to 532,214
Rv0442c
Rv0443: Rv0443 - Conserved protein, at 532,396 to 532,911
Rv0443
Rv0444c: Rv0444c - Anti-sigma factor RskA (regulator of sigma K), at 533,091 to 533,789
Rv0444c
Rv0445c: Rv0445c - Alternative RNA polymerase sigma factor SigK, at 533,833 to 534,396
Rv0445c
Rv0446c: Rv0446c - Possible conserved transmembrane protein, at 534,445 to 535,215
Rv0446c
Position (kb) 533
534 Strain fitness (log2 ratio) -2
-1
0
1 at 532.267 kb on + strand at 532.267 kb on + strand at 532.267 kb on + strand at 532.267 kb on + strand at 532.318 kb on + strand at 532.318 kb on + strand at 532.319 kb on - strand at 532.322 kb on + strand at 532.323 kb on - strand at 532.323 kb on - strand at 532.395 kb on + strand at 532.396 kb on - strand at 532.396 kb on - strand at 532.569 kb on + strand, within Rv0443 at 532.570 kb on - strand, within Rv0443 at 532.570 kb on - strand, within Rv0443 at 532.571 kb on + strand, within Rv0443 at 532.572 kb on - strand, within Rv0443 at 532.572 kb on - strand, within Rv0443 at 532.641 kb on - strand, within Rv0443 at 532.667 kb on - strand, within Rv0443 at 532.727 kb on - strand, within Rv0443 at 532.729 kb on + strand, within Rv0443 at 532.744 kb on - strand, within Rv0443 at 532.744 kb on - strand, within Rv0443 at 532.759 kb on + strand, within Rv0443 at 532.760 kb on - strand, within Rv0443 at 532.760 kb on - strand, within Rv0443 at 532.888 kb on + strand at 532.888 kb on + strand at 532.901 kb on + strand at 532.978 kb on - strand at 532.978 kb on - strand at 533.400 kb on + strand, within Rv0444c at 533.400 kb on + strand, within Rv0444c at 533.401 kb on - strand, within Rv0444c at 533.401 kb on - strand, within Rv0444c at 533.401 kb on - strand, within Rv0444c at 533.522 kb on + strand, within Rv0444c at 533.743 kb on + strand at 533.744 kb on - strand at 533.751 kb on - strand at 533.933 kb on + strand, within Rv0445c at 533.933 kb on + strand, within Rv0445c at 533.934 kb on - strand, within Rv0445c at 533.934 kb on - strand, within Rv0445c at 533.951 kb on + strand, within Rv0445c at 533.951 kb on + strand, within Rv0445c at 533.952 kb on - strand, within Rv0445c at 533.952 kb on - strand, within Rv0445c at 533.952 kb on - strand, within Rv0445c at 533.952 kb on - strand, within Rv0445c at 534.084 kb on - strand, within Rv0445c at 534.248 kb on + strand, within Rv0445c at 534.285 kb on - strand, within Rv0445c at 534.285 kb on - strand, within Rv0445c at 534.309 kb on - strand, within Rv0445c at 534.483 kb on - strand at 534.530 kb on + strand, within Rv0446c at 534.668 kb on + strand, within Rv0446c at 534.669 kb on - strand, within Rv0446c at 534.742 kb on + strand, within Rv0446c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Linezolid 0.44 ug/mL remove 532,267 + +0.5 532,267 + -1.8 532,267 + -1.0 532,267 + -0.3 532,318 + +1.4 532,318 + +0.7 532,319 - -0.3 532,322 + -0.6 532,323 - -0.8 532,323 - -1.1 532,395 + -0.6 532,396 - -2.7 532,396 - -0.8 532,569 + Rv0443 0.34 -0.5 532,570 - Rv0443 0.34 -1.9 532,570 - Rv0443 0.34 -0.7 532,571 + Rv0443 0.34 +0.6 532,572 - Rv0443 0.34 +0.5 532,572 - Rv0443 0.34 +0.5 532,641 - Rv0443 0.47 -0.4 532,667 - Rv0443 0.53 -0.2 532,727 - Rv0443 0.64 -0.3 532,729 + Rv0443 0.65 -0.3 532,744 - Rv0443 0.67 -0.1 532,744 - Rv0443 0.67 -0.3 532,759 + Rv0443 0.70 +1.2 532,760 - Rv0443 0.71 +0.5 532,760 - Rv0443 0.71 +0.2 532,888 + +0.1 532,888 + -0.6 532,901 + -0.3 532,978 - +0.5 532,978 - -0.4 533,400 + Rv0444c 0.44 -2.0 533,400 + Rv0444c 0.44 -1.0 533,401 - Rv0444c 0.44 -0.6 533,401 - Rv0444c 0.44 -1.4 533,401 - Rv0444c 0.44 -0.4 533,522 + Rv0444c 0.62 -1.0 533,743 + +0.2 533,744 - -2.4 533,751 - -2.2 533,933 + Rv0445c 0.18 -0.3 533,933 + Rv0445c 0.18 -0.8 533,934 - Rv0445c 0.18 -0.8 533,934 - Rv0445c 0.18 -0.3 533,951 + Rv0445c 0.21 +0.0 533,951 + Rv0445c 0.21 -0.5 533,952 - Rv0445c 0.21 -1.1 533,952 - Rv0445c 0.21 -0.3 533,952 - Rv0445c 0.21 +0.1 533,952 - Rv0445c 0.21 +0.2 534,084 - Rv0445c 0.45 -0.5 534,248 + Rv0445c 0.74 -0.4 534,285 - Rv0445c 0.80 -0.5 534,285 - Rv0445c 0.80 +0.8 534,309 - Rv0445c 0.84 +0.0 534,483 - -0.5 534,530 + Rv0446c 0.11 -0.8 534,668 + Rv0446c 0.29 -0.2 534,669 - Rv0446c 0.29 -0.7 534,742 + Rv0446c 0.39 -0.6
Or see this region's nucleotide sequence