Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3032A

Experiment: 7H9 with Linezolid 0.3 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3032 and Rv3032A are separated by 33 nucleotidesRv3032A and Rv3033 are separated by 178 nucleotidesRv3033 and Rv3034c are separated by 90 nucleotides Rv3032: Rv3032 - Alpha (1->4) glucosyltransferase, at 3,391,534 to 3,392,778 Rv3032 Rv3032A: Rv3032A - Conserved protein, at 3,392,812 to 3,393,201 Rv3032A Rv3033: Rv3033 - Unknown protein, at 3,393,380 to 3,393,928 Rv3033 Rv3034c: Rv3034c - Possible transferase, at 3,394,019 to 3,394,921 Rv3034c Position (kb) 3392 3393 3394Strain fitness (log2 ratio) -1 0 1at 3392.777 kb on - strandat 3392.879 kb on + strand, within Rv3032Aat 3393.793 kb on - strand, within Rv3033

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Linezolid 0.3 ug/mL
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3,392,777 - +1.7
3,392,879 + Rv3032A 0.17 +0.3
3,393,793 - Rv3033 0.75 -1.2

Or see this region's nucleotide sequence