Experiment: 7H9 with Linezolid 0.3 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0440 and Rv0441c are separated by 65 nucleotides Rv0441c and Rv0442c are separated by 26 nucleotides Rv0442c and Rv0443 are separated by 181 nucleotides Rv0443 and Rv0444c are separated by 179 nucleotides
Rv0440: Rv0440 - 60 kDa chaperonin 2 GroEL2 (protein CPN60-2) (GroEL protein 2) (65 kDa antigen) (heat shock protein 65) (cell wall protein A) (antigen A), at 528,608 to 530,230
Rv0440
Rv0441c: Rv0441c - Hypothetical protein, at 530,296 to 530,724
Rv0441c
Rv0442c: Rv0442c - PPE family protein PPE10, at 530,751 to 532,214
Rv0442c
Rv0443: Rv0443 - Conserved protein, at 532,396 to 532,911
Rv0443
Rv0444c: Rv0444c - Anti-sigma factor RskA (regulator of sigma K), at 533,091 to 533,789
Rv0444c
Position (kb) 530
531
532
533 Strain fitness (log2 ratio) -2
-1
0
1
2 at 530.297 kb on + strand at 530.297 kb on + strand at 530.297 kb on + strand at 530.298 kb on - strand at 530.298 kb on - strand at 530.298 kb on - strand at 530.326 kb on + strand at 530.326 kb on + strand at 530.327 kb on - strand at 530.469 kb on - strand, within Rv0441c at 530.475 kb on + strand, within Rv0441c at 530.475 kb on + strand, within Rv0441c at 530.535 kb on + strand, within Rv0441c at 530.536 kb on - strand, within Rv0441c at 530.672 kb on + strand, within Rv0441c at 530.708 kb on - strand at 530.708 kb on - strand at 530.736 kb on + strand at 530.736 kb on + strand at 530.792 kb on - strand at 530.818 kb on + strand at 530.819 kb on - strand at 530.852 kb on + strand at 530.853 kb on - strand at 530.921 kb on - strand, within Rv0442c at 530.939 kb on + strand, within Rv0442c at 530.939 kb on + strand, within Rv0442c at 530.940 kb on - strand, within Rv0442c at 530.940 kb on - strand, within Rv0442c at 530.940 kb on - strand, within Rv0442c at 531.166 kb on + strand, within Rv0442c at 531.167 kb on - strand, within Rv0442c at 531.214 kb on - strand, within Rv0442c at 531.363 kb on + strand, within Rv0442c at 531.363 kb on + strand, within Rv0442c at 531.787 kb on + strand, within Rv0442c at 531.839 kb on + strand, within Rv0442c at 531.877 kb on - strand, within Rv0442c at 531.987 kb on + strand, within Rv0442c at 531.988 kb on - strand, within Rv0442c at 531.988 kb on - strand, within Rv0442c at 531.992 kb on - strand, within Rv0442c at 532.267 kb on + strand at 532.267 kb on + strand at 532.267 kb on + strand at 532.267 kb on + strand at 532.318 kb on + strand at 532.318 kb on + strand at 532.319 kb on - strand at 532.322 kb on + strand at 532.323 kb on - strand at 532.323 kb on - strand at 532.395 kb on + strand at 532.396 kb on - strand at 532.396 kb on - strand at 532.569 kb on + strand, within Rv0443 at 532.570 kb on - strand, within Rv0443 at 532.570 kb on - strand, within Rv0443 at 532.571 kb on + strand, within Rv0443 at 532.572 kb on - strand, within Rv0443 at 532.572 kb on - strand, within Rv0443 at 532.641 kb on - strand, within Rv0443 at 532.667 kb on - strand, within Rv0443 at 532.727 kb on - strand, within Rv0443 at 532.729 kb on + strand, within Rv0443 at 532.744 kb on - strand, within Rv0443 at 532.744 kb on - strand, within Rv0443 at 532.759 kb on + strand, within Rv0443 at 532.760 kb on - strand, within Rv0443 at 532.760 kb on - strand, within Rv0443 at 532.888 kb on + strand at 532.888 kb on + strand at 532.901 kb on + strand at 532.978 kb on - strand at 532.978 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Linezolid 0.3 ug/mL remove 530,297 + -0.6 530,297 + -0.3 530,297 + -0.4 530,298 - +0.8 530,298 - -1.4 530,298 - +0.6 530,326 + -0.7 530,326 + -0.5 530,327 - +0.9 530,469 - Rv0441c 0.40 +1.6 530,475 + Rv0441c 0.42 -0.5 530,475 + Rv0441c 0.42 -0.1 530,535 + Rv0441c 0.56 -0.3 530,536 - Rv0441c 0.56 -0.5 530,672 + Rv0441c 0.88 +0.4 530,708 - -0.8 530,708 - +0.6 530,736 + -0.9 530,736 + -0.4 530,792 - -0.4 530,818 + -0.8 530,819 - -0.8 530,852 + -0.2 530,853 - +0.6 530,921 - Rv0442c 0.12 +1.0 530,939 + Rv0442c 0.13 -0.1 530,939 + Rv0442c 0.13 -0.7 530,940 - Rv0442c 0.13 +1.6 530,940 - Rv0442c 0.13 +0.1 530,940 - Rv0442c 0.13 +0.2 531,166 + Rv0442c 0.28 -0.5 531,167 - Rv0442c 0.28 -0.4 531,214 - Rv0442c 0.32 -0.5 531,363 + Rv0442c 0.42 -1.2 531,363 + Rv0442c 0.42 -0.4 531,787 + Rv0442c 0.71 -1.2 531,839 + Rv0442c 0.74 +0.3 531,877 - Rv0442c 0.77 -1.4 531,987 + Rv0442c 0.84 -1.1 531,988 - Rv0442c 0.84 +0.2 531,988 - Rv0442c 0.84 +0.6 531,992 - Rv0442c 0.85 +0.4 532,267 + -0.2 532,267 + +1.9 532,267 + +0.0 532,267 + -0.6 532,318 + +0.9 532,318 + -0.2 532,319 - -0.0 532,322 + -0.2 532,323 - -1.4 532,323 - -0.3 532,395 + -0.2 532,396 - -1.7 532,396 - -0.3 532,569 + Rv0443 0.34 +0.0 532,570 - Rv0443 0.34 -2.1 532,570 - Rv0443 0.34 -0.4 532,571 + Rv0443 0.34 -0.1 532,572 - Rv0443 0.34 +0.1 532,572 - Rv0443 0.34 +0.9 532,641 - Rv0443 0.47 -1.4 532,667 - Rv0443 0.53 -0.1 532,727 - Rv0443 0.64 -0.2 532,729 + Rv0443 0.65 -0.2 532,744 - Rv0443 0.67 -0.6 532,744 - Rv0443 0.67 -0.0 532,759 + Rv0443 0.70 -0.2 532,760 - Rv0443 0.71 -0.5 532,760 - Rv0443 0.71 -0.5 532,888 + -2.1 532,888 + -0.2 532,901 + +0.1 532,978 - +0.3 532,978 - -0.4
Or see this region's nucleotide sequence