Experiment: 7H9 with Linezolid 0.3 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0064 and Rv0064A are separated by 29 nucleotides Rv0064A and Rv0065 overlap by 8 nucleotides Rv0065 and Rv0066c are separated by 51 nucleotides
Rv0064: Rv0064 - Probable conserved transmembrane protein, at 68,620 to 71,559
Rv0064
Rv0064A: Rv0064A - Possible antitoxin VapB1, at 71,589 to 71,828
Rv0064A
Rv0065: Rv0065 - Possible toxin VapC1, at 71,821 to 72,222
Rv0065
Rv0066c: Rv0066c - Probable isocitrate dehydrogenase [NADP] Icd2 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP), at 72,274 to 74,511
Rv0066c
Position (kb) 71
72 Strain fitness (log2 ratio) -2
-1
0
1
2 at 70.728 kb on + strand, within Rv0064 at 70.933 kb on + strand, within Rv0064 at 70.988 kb on + strand, within Rv0064 at 71.143 kb on + strand, within Rv0064 at 71.143 kb on + strand, within Rv0064 at 71.144 kb on - strand, within Rv0064 at 71.144 kb on - strand, within Rv0064 at 71.300 kb on + strand at 71.679 kb on + strand, within Rv0064A at 71.679 kb on + strand, within Rv0064A at 71.679 kb on + strand, within Rv0064A at 71.679 kb on + strand, within Rv0064A at 71.680 kb on - strand, within Rv0064A at 71.680 kb on - strand, within Rv0064A at 71.680 kb on - strand, within Rv0064A at 71.834 kb on + strand at 71.835 kb on - strand at 72.032 kb on - strand, within Rv0065 at 72.077 kb on - strand, within Rv0065 at 72.089 kb on + strand, within Rv0065
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Linezolid 0.3 ug/mL remove 70,728 + Rv0064 0.72 -1.2 70,933 + Rv0064 0.79 -0.4 70,988 + Rv0064 0.81 +0.3 71,143 + Rv0064 0.86 +0.6 71,143 + Rv0064 0.86 +0.2 71,144 - Rv0064 0.86 +0.0 71,144 - Rv0064 0.86 -1.0 71,300 + +0.4 71,679 + Rv0064A 0.38 +0.4 71,679 + Rv0064A 0.38 -0.0 71,679 + Rv0064A 0.38 +2.1 71,679 + Rv0064A 0.38 -0.7 71,680 - Rv0064A 0.38 -0.2 71,680 - Rv0064A 0.38 +1.0 71,680 - Rv0064A 0.38 -1.9 71,834 + -0.0 71,835 - -0.5 72,032 - Rv0065 0.52 -0.2 72,077 - Rv0065 0.64 +1.0 72,089 + Rv0065 0.67 +1.1
Or see this region's nucleotide sequence