Experiment: 7H9 with Linezolid 0.3 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0938 and Rv0939 overlap by 4 nucleotides Rv0939 and Rv0940c are separated by 246 nucleotides
Rv0938: Rv0938 - ATP dependent DNA ligase LigD (ATP dependent polydeoxyribonucleotide synthase) (thermostable DNA ligase) (ATP dependent polynucleotide ligase) (sealase) (DNA repair enzyme) (DNA joinase), at 1,046,136 to 1,048,415
Rv0938
Rv0939: Rv0939 - Possible bifunctional enzyme: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HHDD isomerase) + cyclase/dehydrase, at 1,048,412 to 1,050,346
Rv0939
Rv0940c: Rv0940c - Possible oxidoreductase, at 1,050,593 to 1,051,459
Rv0940c
Position (kb) 1048
1049
1050
1051 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1048.005 kb on + strand, within Rv0938 at 1048.255 kb on + strand at 1048.290 kb on + strand at 1048.291 kb on - strand at 1048.479 kb on - strand at 1049.136 kb on - strand, within Rv0939 at 1049.525 kb on - strand, within Rv0939 at 1050.248 kb on + strand at 1050.248 kb on + strand at 1051.318 kb on - strand, within Rv0940c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Linezolid 0.3 ug/mL remove 1,048,005 + Rv0938 0.82 +0.0 1,048,255 + +0.2 1,048,290 + -0.2 1,048,291 - -0.3 1,048,479 - +0.9 1,049,136 - Rv0939 0.37 -2.0 1,049,525 - Rv0939 0.58 +2.3 1,050,248 + +1.2 1,050,248 + -0.1 1,051,318 - Rv0940c 0.84 +0.0
Or see this region's nucleotide sequence