Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0627

Experiment: 7H9 with Linezolid 0.3 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0625c and Rv0626 are separated by 131 nucleotidesRv0626 and Rv0627 overlap by 4 nucleotidesRv0627 and Rv0628c are separated by 71 nucleotides Rv0625c: Rv0625c - Probable conserved transmembrane protein, at 717,153 to 717,893 Rv0625c Rv0626: Rv0626 - Possible antitoxin VapB5, at 718,025 to 718,285 Rv0626 Rv0627: Rv0627 - Possible toxin VapC5, at 718,282 to 718,689 Rv0627 Rv0628c: Rv0628c - Conserved hypothetical protein, at 718,761 to 719,912 Rv0628c Position (kb) 718 719Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 717.428 kb on - strand, within Rv0625cat 717.428 kb on - strand, within Rv0625cat 717.718 kb on + strand, within Rv0625cat 717.719 kb on - strand, within Rv0625cat 717.719 kb on - strand, within Rv0625cat 717.802 kb on + strand, within Rv0625cat 717.803 kb on - strand, within Rv0625cat 717.803 kb on - strand, within Rv0625cat 717.813 kb on + strand, within Rv0625cat 717.813 kb on + strand, within Rv0625cat 718.001 kb on + strandat 718.020 kb on + strandat 718.054 kb on + strand, within Rv0626at 719.305 kb on + strand, within Rv0628cat 719.305 kb on + strand, within Rv0628cat 719.305 kb on + strand, within Rv0628cat 719.306 kb on - strand, within Rv0628cat 719.306 kb on - strand, within Rv0628cat 719.306 kb on - strand, within Rv0628cat 719.522 kb on - strand, within Rv0628c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Linezolid 0.3 ug/mL
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717,428 - Rv0625c 0.37 -0.4
717,428 - Rv0625c 0.37 +2.7
717,718 + Rv0625c 0.76 -1.8
717,719 - Rv0625c 0.76 -0.1
717,719 - Rv0625c 0.76 +0.3
717,802 + Rv0625c 0.88 -0.0
717,803 - Rv0625c 0.88 -0.5
717,803 - Rv0625c 0.88 -0.5
717,813 + Rv0625c 0.89 -0.5
717,813 + Rv0625c 0.89 +4.3
718,001 + -0.2
718,020 + +0.8
718,054 + Rv0626 0.11 +0.1
719,305 + Rv0628c 0.47 -0.4
719,305 + Rv0628c 0.47 -0.2
719,305 + Rv0628c 0.47 +1.1
719,306 - Rv0628c 0.47 -0.0
719,306 - Rv0628c 0.47 +0.4
719,306 - Rv0628c 0.47 -1.8
719,522 - Rv0628c 0.66 +0.2

Or see this region's nucleotide sequence