Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2467

Experiment: 7H9 with Malondialdehyde tetrabutylammonium salt 0.96 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2465c and Rv2466c are separated by 101 nucleotidesRv2466c and Rv2467 are separated by 101 nucleotidesRv2467 and Rv2468c are separated by 72 nucleotidesRv2468c and Rv2468A overlap by 50 nucleotidesRv2468A and Rv2469c are separated by 35 nucleotides Rv2465c: Rv2465c - Ribose-5-phosphate isomerase, at 2,767,671 to 2,768,159 Rv2465c Rv2466c: Rv2466c - Conserved protein, at 2,768,261 to 2,768,884 Rv2466c Rv2467: Rv2467 - Probable aminopeptidase N PepN (Lysyl aminopeptidase) (LYS-AP) (alanine aminopeptidase), at 2,768,986 to 2,771,571 Rv2467 Rv2468c: Rv2468c - Conserved protein, at 2,771,644 to 2,772,147 Rv2468c Rv2468A: Rv2468A - Conserved protein, at 2,772,098 to 2,772,331 Rv2468A Rv2469c: Rv2469c - Conserved hypothetical protein, at 2,772,367 to 2,773,035 Rv2469c Position (kb) 2768 2769 2770 2771 2772Strain fitness (log2 ratio) -2 -1 0 1 2at 2768.262 kb on + strandat 2768.316 kb on + strandat 2768.329 kb on + strand, within Rv2466cat 2768.330 kb on - strand, within Rv2466cat 2768.330 kb on - strand, within Rv2466cat 2768.429 kb on - strand, within Rv2466cat 2768.613 kb on + strand, within Rv2466cat 2768.738 kb on + strand, within Rv2466cat 2768.920 kb on + strandat 2769.052 kb on + strandat 2769.053 kb on - strandat 2769.301 kb on + strand, within Rv2467at 2769.358 kb on + strand, within Rv2467at 2769.529 kb on - strand, within Rv2467at 2769.529 kb on - strand, within Rv2467at 2769.595 kb on + strand, within Rv2467at 2769.596 kb on - strand, within Rv2467at 2769.656 kb on - strand, within Rv2467at 2769.706 kb on + strand, within Rv2467at 2769.853 kb on + strand, within Rv2467at 2770.139 kb on - strand, within Rv2467at 2770.216 kb on + strand, within Rv2467at 2770.216 kb on + strand, within Rv2467at 2770.606 kb on + strand, within Rv2467at 2770.607 kb on - strand, within Rv2467at 2770.945 kb on + strand, within Rv2467at 2770.947 kb on + strand, within Rv2467at 2770.947 kb on + strand, within Rv2467at 2771.045 kb on + strand, within Rv2467at 2771.078 kb on + strand, within Rv2467at 2771.344 kb on + strandat 2771.366 kb on + strandat 2771.417 kb on - strandat 2771.621 kb on + strandat 2771.698 kb on + strand, within Rv2468cat 2771.699 kb on - strand, within Rv2468cat 2771.789 kb on + strand, within Rv2468cat 2771.789 kb on + strand, within Rv2468cat 2771.948 kb on + strand, within Rv2468cat 2771.948 kb on + strand, within Rv2468cat 2771.949 kb on - strand, within Rv2468cat 2772.235 kb on - strand, within Rv2468Aat 2772.235 kb on - strand, within Rv2468Aat 2772.235 kb on - strand, within Rv2468Aat 2772.235 kb on - strand, within Rv2468Aat 2772.331 kb on + strandat 2772.332 kb on - strandat 2772.353 kb on - strandat 2772.389 kb on + strandat 2772.389 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Malondialdehyde tetrabutylammonium salt 0.96 mM
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2,768,262 + -1.0
2,768,316 + -0.5
2,768,329 + Rv2466c 0.11 +0.4
2,768,330 - Rv2466c 0.11 -0.4
2,768,330 - Rv2466c 0.11 +0.1
2,768,429 - Rv2466c 0.27 -0.5
2,768,613 + Rv2466c 0.56 +0.3
2,768,738 + Rv2466c 0.76 -0.9
2,768,920 + +0.3
2,769,052 + -1.4
2,769,053 - -1.9
2,769,301 + Rv2467 0.12 +1.1
2,769,358 + Rv2467 0.14 +0.8
2,769,529 - Rv2467 0.21 -1.1
2,769,529 - Rv2467 0.21 -1.5
2,769,595 + Rv2467 0.24 -1.0
2,769,596 - Rv2467 0.24 -0.5
2,769,656 - Rv2467 0.26 -2.3
2,769,706 + Rv2467 0.28 +0.1
2,769,853 + Rv2467 0.34 -0.9
2,770,139 - Rv2467 0.45 -1.9
2,770,216 + Rv2467 0.48 -0.2
2,770,216 + Rv2467 0.48 +0.2
2,770,606 + Rv2467 0.63 -0.5
2,770,607 - Rv2467 0.63 -0.3
2,770,945 + Rv2467 0.76 -0.8
2,770,947 + Rv2467 0.76 -2.2
2,770,947 + Rv2467 0.76 -0.6
2,771,045 + Rv2467 0.80 -1.6
2,771,078 + Rv2467 0.81 -1.5
2,771,344 + -1.3
2,771,366 + -0.1
2,771,417 - -0.4
2,771,621 + -0.8
2,771,698 + Rv2468c 0.11 +0.5
2,771,699 - Rv2468c 0.11 -0.6
2,771,789 + Rv2468c 0.29 -0.8
2,771,789 + Rv2468c 0.29 -0.1
2,771,948 + Rv2468c 0.60 -0.2
2,771,948 + Rv2468c 0.60 +1.8
2,771,949 - Rv2468c 0.61 -0.5
2,772,235 - Rv2468A 0.59 -1.3
2,772,235 - Rv2468A 0.59 +0.1
2,772,235 - Rv2468A 0.59 -0.9
2,772,235 - Rv2468A 0.59 -1.2
2,772,331 + -0.2
2,772,332 - -0.5
2,772,353 - -0.9
2,772,389 + +0.3
2,772,389 + -0.1

Or see this region's nucleotide sequence