Experiment: 7H9 with Malondialdehyde tetrabutylammonium salt 0.96 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0006 and Rv0007 are separated by 95 nucleotides Rv0007 and GFF8 are separated by 58 nucleotides GFF8 and GFF9 are separated by 151 nucleotides
Rv0006: Rv0006 - DNA gyrase (subunit A) GyrA (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase), at 7,302 to 9,818
Rv0006
Rv0007: Rv0007 - Possible conserved membrane protein, at 9,914 to 10,828
Rv0007
GFF8 - tRNA-Ile, at 10,887 to 10,960
GFF8
GFF9 - tRNA-Ala, at 11,112 to 11,184
GFF9
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 9.817 kb on - strand at 9.877 kb on + strand at 9.877 kb on + strand at 9.878 kb on - strand at 9.878 kb on - strand at 9.878 kb on - strand at 10.278 kb on - strand, within Rv0007 at 11.289 kb on + strand at 11.290 kb on - strand at 11.422 kb on + strand at 11.422 kb on + strand at 11.557 kb on - strand at 11.619 kb on - strand at 11.675 kb on + strand at 11.675 kb on + strand at 11.675 kb on + strand at 11.675 kb on + strand at 11.675 kb on + strand at 11.676 kb on - strand at 11.676 kb on - strand at 11.679 kb on + strand at 11.680 kb on - strand at 11.733 kb on + strand at 11.733 kb on + strand at 11.743 kb on + strand at 11.743 kb on + strand at 11.744 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Malondialdehyde tetrabutylammonium salt 0.96 mM remove 9,817 - +1.9 9,877 + -0.1 9,877 + +0.1 9,878 - +1.9 9,878 - -0.9 9,878 - -0.5 10,278 - Rv0007 0.40 -2.0 11,289 + +0.6 11,290 - -1.1 11,422 + +0.4 11,422 + -0.1 11,557 - +0.4 11,619 - +0.3 11,675 + -1.8 11,675 + -0.2 11,675 + -0.3 11,675 + -0.3 11,675 + -0.0 11,676 - +0.3 11,676 - +0.1 11,679 + -0.0 11,680 - +0.9 11,733 + +0.9 11,733 + -0.5 11,743 + -0.8 11,743 + -0.3 11,744 - +3.2
Or see this region's nucleotide sequence