Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01895 and MMJJ_RS01900 are separated by 144 nucleotides MMJJ_RS01900 and MMJJ_RS09385 are separated by 120 nucleotides MMJJ_RS09385 and MMJJ_RS01910 are separated by 480 nucleotides
MMJJ_RS01895: MMJJ_RS01895 - translation initiation factor eIF-1A, at 363,985 to 364,299
_RS01895
MMJJ_RS01900: MMJJ_RS01900 - orotidine-5'-phosphate decarboxylase, at 364,444 to 365,097
_RS01900
MMJJ_RS09385: MMJJ_RS09385 - class III signal peptide-containing protein, at 365,218 to 365,454
_RS09385
MMJJ_RS01910: MMJJ_RS01910 - class III signal peptide-containing protein, at 365,935 to 366,618
_RS01910
Position (kb) 365
366 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 364.410 kb on + strand at 364.440 kb on + strand at 364.443 kb on - strand at 365.082 kb on - strand at 365.095 kb on - strand at 365.095 kb on - strand at 365.095 kb on - strand at 365.095 kb on - strand at 365.095 kb on - strand at 365.096 kb on + strand at 365.096 kb on + strand at 365.096 kb on + strand at 365.102 kb on - strand at 365.103 kb on + strand at 365.103 kb on + strand at 365.116 kb on - strand at 365.116 kb on - strand at 365.133 kb on + strand at 365.133 kb on + strand at 365.136 kb on - strand at 365.151 kb on - strand at 365.151 kb on - strand at 365.151 kb on - strand at 365.151 kb on - strand at 365.151 kb on - strand at 365.152 kb on + strand at 365.152 kb on + strand at 365.152 kb on + strand at 365.152 kb on + strand at 365.153 kb on - strand at 365.153 kb on - strand at 365.153 kb on - strand at 365.154 kb on + strand at 365.154 kb on + strand at 365.154 kb on + strand at 365.154 kb on + strand at 365.154 kb on + strand at 365.154 kb on + strand at 365.155 kb on - strand at 365.155 kb on - strand at 365.155 kb on - strand at 365.155 kb on - strand at 365.156 kb on + strand at 365.156 kb on + strand at 365.156 kb on + strand at 365.158 kb on - strand at 365.158 kb on - strand at 365.159 kb on + strand at 365.161 kb on - strand at 365.161 kb on - strand at 365.166 kb on - strand at 365.179 kb on - strand at 365.185 kb on - strand at 365.186 kb on + strand at 365.201 kb on + strand at 365.201 kb on + strand at 365.201 kb on + strand at 365.201 kb on + strand at 365.213 kb on - strand at 365.214 kb on + strand at 365.217 kb on - strand at 365.217 kb on - strand at 365.217 kb on - strand at 365.217 kb on - strand at 365.218 kb on + strand at 365.225 kb on - strand at 365.261 kb on - strand, within MMJJ_RS09385 at 365.262 kb on + strand, within MMJJ_RS09385 at 365.264 kb on - strand, within MMJJ_RS09385 at 365.264 kb on - strand, within MMJJ_RS09385 at 365.265 kb on + strand, within MMJJ_RS09385 at 365.265 kb on + strand, within MMJJ_RS09385 at 365.267 kb on - strand, within MMJJ_RS09385 at 365.267 kb on - strand, within MMJJ_RS09385 at 365.267 kb on - strand, within MMJJ_RS09385 at 365.268 kb on + strand, within MMJJ_RS09385 at 365.279 kb on - strand, within MMJJ_RS09385 at 365.279 kb on - strand, within MMJJ_RS09385 at 365.280 kb on + strand, within MMJJ_RS09385 at 365.282 kb on - strand, within MMJJ_RS09385 at 365.282 kb on - strand, within MMJJ_RS09385 at 365.283 kb on + strand, within MMJJ_RS09385 at 365.283 kb on + strand, within MMJJ_RS09385 at 365.297 kb on - strand, within MMJJ_RS09385 at 365.298 kb on + strand, within MMJJ_RS09385 at 365.298 kb on + strand, within MMJJ_RS09385 at 365.322 kb on - strand, within MMJJ_RS09385 at 365.490 kb on - strand at 365.528 kb on - strand at 365.537 kb on + strand at 365.637 kb on + strand at 365.650 kb on - strand at 365.650 kb on - strand at 365.658 kb on - strand at 365.659 kb on + strand at 365.698 kb on + strand at 365.704 kb on - strand at 365.705 kb on + strand at 365.705 kb on + strand at 365.764 kb on - strand at 365.765 kb on + strand at 365.765 kb on + strand at 365.766 kb on - strand at 365.766 kb on - strand at 365.766 kb on - strand at 365.767 kb on + strand at 365.767 kb on + strand at 365.767 kb on + strand at 365.767 kb on + strand at 365.767 kb on + strand at 365.767 kb on + strand at 365.767 kb on + strand at 365.767 kb on + strand at 365.767 kb on + strand at 365.769 kb on - strand at 365.769 kb on - strand at 365.769 kb on - strand at 365.769 kb on - strand at 365.770 kb on + strand at 365.770 kb on + strand at 365.770 kb on + strand at 365.770 kb on + strand at 365.770 kb on + strand at 365.770 kb on + strand at 365.770 kb on + strand at 365.779 kb on + strand at 365.798 kb on - strand at 365.805 kb on + strand at 365.805 kb on + strand at 365.809 kb on - strand at 365.809 kb on - strand at 365.809 kb on - strand at 365.809 kb on - strand at 365.809 kb on - strand at 365.809 kb on - strand at 365.809 kb on - strand at 365.809 kb on - strand at 365.809 kb on - strand at 365.809 kb on - strand at 365.809 kb on - strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.810 kb on + strand at 365.811 kb on - strand at 365.811 kb on - strand at 365.811 kb on - strand at 365.812 kb on + strand at 365.829 kb on - strand at 365.829 kb on - strand at 365.829 kb on - strand at 365.830 kb on + strand at 365.830 kb on + strand at 365.833 kb on - strand at 365.833 kb on - strand at 365.859 kb on - strand at 365.863 kb on - strand at 365.863 kb on - strand at 365.867 kb on - strand at 365.867 kb on - strand at 365.887 kb on + strand at 365.893 kb on - strand at 365.895 kb on - strand at 365.899 kb on - strand at 365.899 kb on - strand at 365.899 kb on - strand at 365.899 kb on - strand at 365.902 kb on - strand at 365.903 kb on + strand at 365.931 kb on + strand at 365.931 kb on + strand at 365.932 kb on - strand at 365.932 kb on - strand at 365.932 kb on - strand at 365.932 kb on - strand at 365.932 kb on - strand at 365.932 kb on - strand at 365.932 kb on - strand at 365.932 kb on - strand at 365.933 kb on + strand at 365.933 kb on + strand at 365.933 kb on + strand at 365.933 kb on + strand at 365.933 kb on + strand at 365.933 kb on + strand at 365.934 kb on - strand at 365.972 kb on - strand at 365.972 kb on - strand at 365.993 kb on - strand at 366.036 kb on - strand, within MMJJ_RS01910 at 366.036 kb on - strand, within MMJJ_RS01910 at 366.053 kb on - strand, within MMJJ_RS01910 at 366.053 kb on - strand, within MMJJ_RS01910 at 366.053 kb on - strand, within MMJJ_RS01910 at 366.054 kb on + strand, within MMJJ_RS01910 at 366.054 kb on + strand, within MMJJ_RS01910 at 366.054 kb on + strand, within MMJJ_RS01910 at 366.057 kb on + strand, within MMJJ_RS01910 at 366.079 kb on - strand, within MMJJ_RS01910 at 366.079 kb on - strand, within MMJJ_RS01910 at 366.080 kb on + strand, within MMJJ_RS01910 at 366.080 kb on + strand, within MMJJ_RS01910 at 366.086 kb on - strand, within MMJJ_RS01910 at 366.094 kb on + strand, within MMJJ_RS01910 at 366.094 kb on + strand, within MMJJ_RS01910 at 366.096 kb on + strand, within MMJJ_RS01910 at 366.101 kb on - strand, within MMJJ_RS01910 at 366.101 kb on - strand, within MMJJ_RS01910 at 366.102 kb on + strand, within MMJJ_RS01910 at 366.102 kb on + strand, within MMJJ_RS01910 at 366.102 kb on + strand, within MMJJ_RS01910 at 366.102 kb on + strand, within MMJJ_RS01910 at 366.102 kb on + strand, within MMJJ_RS01910 at 366.103 kb on - strand, within MMJJ_RS01910 at 366.103 kb on - strand, within MMJJ_RS01910 at 366.103 kb on - strand, within MMJJ_RS01910 at 366.104 kb on + strand, within MMJJ_RS01910 at 366.104 kb on + strand, within MMJJ_RS01910 at 366.104 kb on + strand, within MMJJ_RS01910 at 366.104 kb on + strand, within MMJJ_RS01910 at 366.104 kb on + strand, within MMJJ_RS01910 at 366.117 kb on + strand, within MMJJ_RS01910 at 366.118 kb on - strand, within MMJJ_RS01910 at 366.118 kb on - strand, within MMJJ_RS01910 at 366.118 kb on - strand, within MMJJ_RS01910 at 366.118 kb on - strand, within MMJJ_RS01910 at 366.119 kb on + strand, within MMJJ_RS01910 at 366.119 kb on + strand, within MMJJ_RS01910 at 366.122 kb on + strand, within MMJJ_RS01910 at 366.207 kb on - strand, within MMJJ_RS01910 at 366.208 kb on + strand, within MMJJ_RS01910 at 366.211 kb on - strand, within MMJJ_RS01910 at 366.211 kb on - strand, within MMJJ_RS01910 at 366.212 kb on + strand, within MMJJ_RS01910 at 366.212 kb on + strand, within MMJJ_RS01910 at 366.212 kb on + strand, within MMJJ_RS01910 at 366.212 kb on + strand, within MMJJ_RS01910 at 366.221 kb on + strand, within MMJJ_RS01910 at 366.241 kb on + strand, within MMJJ_RS01910 at 366.300 kb on + strand, within MMJJ_RS01910 at 366.327 kb on - strand, within MMJJ_RS01910 at 366.328 kb on + strand, within MMJJ_RS01910 at 366.329 kb on - strand, within MMJJ_RS01910 at 366.329 kb on - strand, within MMJJ_RS01910 at 366.330 kb on + strand, within MMJJ_RS01910 at 366.330 kb on + strand, within MMJJ_RS01910 at 366.345 kb on - strand, within MMJJ_RS01910 at 366.374 kb on + strand, within MMJJ_RS01910 at 366.394 kb on + strand, within MMJJ_RS01910 at 366.396 kb on - strand, within MMJJ_RS01910 at 366.440 kb on - strand, within MMJJ_RS01910 at 366.445 kb on - strand, within MMJJ_RS01910 at 366.446 kb on + strand, within MMJJ_RS01910
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 364,410 + -1.9 364,440 + -1.6 364,443 - -3.5 365,082 - -1.6 365,095 - +1.1 365,095 - -0.3 365,095 - -0.1 365,095 - +0.0 365,095 - +1.4 365,096 + -3.0 365,096 + -4.3 365,096 + -0.4 365,102 - +0.3 365,103 + -0.0 365,103 + -0.3 365,116 - +1.2 365,116 - -0.9 365,133 + +0.7 365,133 + +0.2 365,136 - -0.3 365,151 - +0.1 365,151 - +1.6 365,151 - -0.3 365,151 - +0.1 365,151 - +0.7 365,152 + +1.7 365,152 + -0.8 365,152 + -1.2 365,152 + +0.5 365,153 - -1.7 365,153 - -0.8 365,153 - -0.6 365,154 + +0.3 365,154 + +0.4 365,154 + -0.0 365,154 + +0.5 365,154 + -0.2 365,154 + -0.1 365,155 - +1.3 365,155 - +0.4 365,155 - +0.9 365,155 - +0.4 365,156 + -1.8 365,156 + -1.2 365,156 + +0.1 365,158 - -0.4 365,158 - -0.4 365,159 + -0.0 365,161 - +0.7 365,161 - +1.2 365,166 - +0.4 365,179 - -0.2 365,185 - -1.1 365,186 + -1.7 365,201 + -0.0 365,201 + +0.5 365,201 + +0.3 365,201 + +0.1 365,213 - +0.5 365,214 + +0.2 365,217 - -1.0 365,217 - +1.1 365,217 - +0.6 365,217 - +0.5 365,218 + +0.7 365,225 - +0.5 365,261 - MMJJ_RS09385 0.18 -0.5 365,262 + MMJJ_RS09385 0.19 +1.0 365,264 - MMJJ_RS09385 0.19 +0.5 365,264 - MMJJ_RS09385 0.19 -0.2 365,265 + MMJJ_RS09385 0.20 +1.7 365,265 + MMJJ_RS09385 0.20 -0.1 365,267 - MMJJ_RS09385 0.21 +0.5 365,267 - MMJJ_RS09385 0.21 +0.8 365,267 - MMJJ_RS09385 0.21 +0.7 365,268 + MMJJ_RS09385 0.21 -0.0 365,279 - MMJJ_RS09385 0.26 -0.2 365,279 - MMJJ_RS09385 0.26 +0.2 365,280 + MMJJ_RS09385 0.26 +0.2 365,282 - MMJJ_RS09385 0.27 -0.8 365,282 - MMJJ_RS09385 0.27 +0.1 365,283 + MMJJ_RS09385 0.27 +1.5 365,283 + MMJJ_RS09385 0.27 -0.6 365,297 - MMJJ_RS09385 0.33 -0.3 365,298 + MMJJ_RS09385 0.34 +0.7 365,298 + MMJJ_RS09385 0.34 +0.3 365,322 - MMJJ_RS09385 0.44 -0.7 365,490 - +0.3 365,528 - -0.8 365,537 + +0.6 365,637 + -0.3 365,650 - -1.2 365,650 - -0.3 365,658 - +1.6 365,659 + +0.3 365,698 + +0.3 365,704 - -0.1 365,705 + -0.0 365,705 + +1.2 365,764 - +0.9 365,765 + -0.2 365,765 + +0.4 365,766 - -1.1 365,766 - -2.0 365,766 - -0.8 365,767 + +0.3 365,767 + +0.6 365,767 + -1.1 365,767 + -0.8 365,767 + -0.4 365,767 + +0.2 365,767 + -1.8 365,767 + -2.4 365,767 + +0.7 365,769 - +0.3 365,769 - -0.8 365,769 - -1.5 365,769 - +0.6 365,770 + -0.2 365,770 + +0.3 365,770 + -0.4 365,770 + +0.3 365,770 + +0.5 365,770 + -0.6 365,770 + +1.1 365,779 + +0.0 365,798 - +0.4 365,805 + -0.3 365,805 + -0.2 365,809 - -0.5 365,809 - -0.4 365,809 - -0.8 365,809 - +0.4 365,809 - +0.8 365,809 - +0.3 365,809 - -1.8 365,809 - +0.2 365,809 - -0.1 365,809 - -3.2 365,809 - +0.2 365,810 + -0.7 365,810 + +0.3 365,810 + +0.2 365,810 + +0.4 365,810 + +0.0 365,810 + +0.4 365,810 + +0.3 365,810 + -0.8 365,810 + -0.8 365,810 + -0.7 365,810 + -2.0 365,810 + -1.2 365,810 + -0.1 365,810 + +0.0 365,810 + +0.7 365,811 - +0.0 365,811 - +0.7 365,811 - -1.1 365,812 + -4.0 365,829 - +0.9 365,829 - +0.7 365,829 - -0.4 365,830 + +0.4 365,830 + +0.6 365,833 - +0.4 365,833 - +0.2 365,859 - -1.0 365,863 - +1.0 365,863 - +0.5 365,867 - +1.0 365,867 - -0.0 365,887 + -0.1 365,893 - +0.7 365,895 - -0.7 365,899 - -0.4 365,899 - -2.4 365,899 - -0.7 365,899 - +1.1 365,902 - +0.6 365,903 + +0.3 365,931 + -0.4 365,931 + -0.2 365,932 - -2.5 365,932 - +0.4 365,932 - -0.2 365,932 - -2.5 365,932 - +1.2 365,932 - -0.2 365,932 - +1.1 365,932 - +0.3 365,933 + -0.2 365,933 + -0.4 365,933 + +0.6 365,933 + -0.7 365,933 + -0.5 365,933 + -0.5 365,934 - -0.9 365,972 - -4.5 365,972 - -0.7 365,993 - +0.3 366,036 - MMJJ_RS01910 0.15 +0.0 366,036 - MMJJ_RS01910 0.15 +1.9 366,053 - MMJJ_RS01910 0.17 -0.7 366,053 - MMJJ_RS01910 0.17 -2.0 366,053 - MMJJ_RS01910 0.17 +0.0 366,054 + MMJJ_RS01910 0.17 +0.7 366,054 + MMJJ_RS01910 0.17 -2.2 366,054 + MMJJ_RS01910 0.17 +0.1 366,057 + MMJJ_RS01910 0.18 -3.7 366,079 - MMJJ_RS01910 0.21 +0.8 366,079 - MMJJ_RS01910 0.21 -3.0 366,080 + MMJJ_RS01910 0.21 +0.1 366,080 + MMJJ_RS01910 0.21 -0.3 366,086 - MMJJ_RS01910 0.22 -3.1 366,094 + MMJJ_RS01910 0.23 +0.3 366,094 + MMJJ_RS01910 0.23 +0.9 366,096 + MMJJ_RS01910 0.24 -0.6 366,101 - MMJJ_RS01910 0.24 -0.8 366,101 - MMJJ_RS01910 0.24 +0.2 366,102 + MMJJ_RS01910 0.24 +0.5 366,102 + MMJJ_RS01910 0.24 +0.6 366,102 + MMJJ_RS01910 0.24 -3.0 366,102 + MMJJ_RS01910 0.24 +0.6 366,102 + MMJJ_RS01910 0.24 +0.0 366,103 - MMJJ_RS01910 0.25 -0.2 366,103 - MMJJ_RS01910 0.25 -0.5 366,103 - MMJJ_RS01910 0.25 +0.5 366,104 + MMJJ_RS01910 0.25 +1.1 366,104 + MMJJ_RS01910 0.25 +0.6 366,104 + MMJJ_RS01910 0.25 -0.2 366,104 + MMJJ_RS01910 0.25 +0.4 366,104 + MMJJ_RS01910 0.25 -1.1 366,117 + MMJJ_RS01910 0.27 +0.7 366,118 - MMJJ_RS01910 0.27 -1.0 366,118 - MMJJ_RS01910 0.27 -1.3 366,118 - MMJJ_RS01910 0.27 +0.4 366,118 - MMJJ_RS01910 0.27 -0.6 366,119 + MMJJ_RS01910 0.27 +0.5 366,119 + MMJJ_RS01910 0.27 -3.4 366,122 + MMJJ_RS01910 0.27 -0.5 366,207 - MMJJ_RS01910 0.40 +0.1 366,208 + MMJJ_RS01910 0.40 -3.0 366,211 - MMJJ_RS01910 0.40 -2.6 366,211 - MMJJ_RS01910 0.40 +1.0 366,212 + MMJJ_RS01910 0.40 +0.3 366,212 + MMJJ_RS01910 0.40 +0.1 366,212 + MMJJ_RS01910 0.40 +0.2 366,212 + MMJJ_RS01910 0.40 -3.4 366,221 + MMJJ_RS01910 0.42 +0.4 366,241 + MMJJ_RS01910 0.45 +0.3 366,300 + MMJJ_RS01910 0.53 -0.3 366,327 - MMJJ_RS01910 0.57 +0.2 366,328 + MMJJ_RS01910 0.57 +0.2 366,329 - MMJJ_RS01910 0.58 +0.6 366,329 - MMJJ_RS01910 0.58 +0.4 366,330 + MMJJ_RS01910 0.58 +0.2 366,330 + MMJJ_RS01910 0.58 +1.3 366,345 - MMJJ_RS01910 0.60 +0.1 366,374 + MMJJ_RS01910 0.64 +0.2 366,394 + MMJJ_RS01910 0.67 +1.0 366,396 - MMJJ_RS01910 0.67 +1.3 366,440 - MMJJ_RS01910 0.74 -1.4 366,445 - MMJJ_RS01910 0.75 -0.2 366,446 + MMJJ_RS01910 0.75 +0.4
Or see this region's nucleotide sequence