Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01840 and MMJJ_RS01845 are separated by 96 nucleotides MMJJ_RS01845 and MMJJ_RS09265 are separated by 80 nucleotides MMJJ_RS09265 and MMJJ_RS01850 are separated by 8 nucleotides MMJJ_RS01850 and MMJJ_RS01855 are separated by 1 nucleotides
MMJJ_RS01840: MMJJ_RS01840 - flavodoxin family protein, at 353,373 to 353,948
_RS01840
MMJJ_RS01845: MMJJ_RS01845 - acetolactate decarboxylase, at 354,045 to 354,845
_RS01845
MMJJ_RS09265: MMJJ_RS09265 - hypothetical protein, at 354,926 to 355,072
_RS09265
MMJJ_RS01850: MMJJ_RS01850 - RNA 2',3'-cyclic phosphodiesterase, at 355,081 to 355,605
_RS01850
MMJJ_RS01855: MMJJ_RS01855 - MBL fold metallo-hydrolase, at 355,607 to 356,149
_RS01855
Position (kb) 354
355
356 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 353.930 kb on - strand at 353.966 kb on - strand at 353.967 kb on + strand at 353.983 kb on - strand at 354.001 kb on - strand at 354.004 kb on + strand at 354.004 kb on + strand at 354.046 kb on - strand at 354.047 kb on + strand at 354.066 kb on - strand at 354.066 kb on - strand at 354.083 kb on - strand at 354.098 kb on - strand at 354.133 kb on - strand, within MMJJ_RS01845 at 354.209 kb on - strand, within MMJJ_RS01845 at 354.228 kb on - strand, within MMJJ_RS01845 at 354.228 kb on - strand, within MMJJ_RS01845 at 354.228 kb on - strand, within MMJJ_RS01845 at 354.228 kb on - strand at 354.229 kb on + strand, within MMJJ_RS01845 at 354.229 kb on + strand, within MMJJ_RS01845 at 354.421 kb on - strand, within MMJJ_RS01845 at 354.422 kb on + strand, within MMJJ_RS01845 at 354.428 kb on - strand, within MMJJ_RS01845 at 354.457 kb on - strand, within MMJJ_RS01845 at 354.477 kb on + strand, within MMJJ_RS01845 at 354.477 kb on + strand, within MMJJ_RS01845 at 354.496 kb on - strand, within MMJJ_RS01845 at 354.496 kb on - strand, within MMJJ_RS01845 at 354.521 kb on - strand, within MMJJ_RS01845 at 354.521 kb on - strand, within MMJJ_RS01845 at 354.522 kb on + strand, within MMJJ_RS01845 at 354.559 kb on - strand, within MMJJ_RS01845 at 354.559 kb on - strand, within MMJJ_RS01845 at 354.560 kb on + strand, within MMJJ_RS01845 at 354.560 kb on + strand, within MMJJ_RS01845 at 354.560 kb on + strand, within MMJJ_RS01845 at 354.560 kb on + strand, within MMJJ_RS01845 at 354.564 kb on - strand, within MMJJ_RS01845 at 354.564 kb on - strand, within MMJJ_RS01845 at 354.565 kb on + strand, within MMJJ_RS01845 at 354.591 kb on - strand, within MMJJ_RS01845 at 354.591 kb on - strand, within MMJJ_RS01845 at 354.591 kb on - strand, within MMJJ_RS01845 at 354.592 kb on + strand, within MMJJ_RS01845 at 354.640 kb on + strand, within MMJJ_RS01845 at 354.679 kb on + strand, within MMJJ_RS01845 at 354.691 kb on - strand, within MMJJ_RS01845 at 354.791 kb on - strand at 354.791 kb on - strand at 354.791 kb on - strand at 354.804 kb on - strand at 354.813 kb on - strand at 354.813 kb on - strand at 354.816 kb on + strand at 354.819 kb on - strand at 354.840 kb on + strand at 354.846 kb on + strand at 354.877 kb on + strand at 354.877 kb on + strand at 354.877 kb on + strand at 354.878 kb on - strand at 354.878 kb on - strand at 354.878 kb on - strand at 354.879 kb on + strand at 354.879 kb on + strand at 354.882 kb on - strand at 354.883 kb on + strand at 354.883 kb on + strand at 354.892 kb on - strand at 354.896 kb on - strand at 354.897 kb on + strand at 354.900 kb on + strand at 354.915 kb on + strand at 354.920 kb on - strand at 354.921 kb on + strand at 355.006 kb on + strand, within MMJJ_RS09265 at 355.039 kb on - strand, within MMJJ_RS09265 at 355.098 kb on - strand at 355.119 kb on + strand at 355.318 kb on - strand, within MMJJ_RS01850 at 355.318 kb on - strand, within MMJJ_RS01850 at 355.318 kb on - strand, within MMJJ_RS01850 at 355.319 kb on + strand, within MMJJ_RS01850 at 355.366 kb on + strand, within MMJJ_RS01850 at 355.447 kb on + strand, within MMJJ_RS01850 at 355.487 kb on - strand, within MMJJ_RS01850 at 355.507 kb on + strand, within MMJJ_RS01850 at 355.529 kb on - strand, within MMJJ_RS01850 at 355.530 kb on + strand, within MMJJ_RS01850 at 355.546 kb on + strand, within MMJJ_RS01850 at 355.546 kb on + strand, within MMJJ_RS01850 at 355.554 kb on - strand at 355.564 kb on + strand at 355.579 kb on - strand at 355.614 kb on - strand at 355.627 kb on + strand at 355.651 kb on - strand at 355.651 kb on - strand at 355.652 kb on + strand at 355.673 kb on - strand, within MMJJ_RS01855 at 355.723 kb on - strand, within MMJJ_RS01855 at 355.724 kb on + strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.780 kb on + strand, within MMJJ_RS01855 at 355.780 kb on + strand, within MMJJ_RS01855 at 355.801 kb on - strand, within MMJJ_RS01855 at 355.853 kb on + strand, within MMJJ_RS01855 at 355.862 kb on - strand, within MMJJ_RS01855 at 355.879 kb on + strand, within MMJJ_RS01855 at 355.882 kb on - strand, within MMJJ_RS01855 at 355.901 kb on + strand, within MMJJ_RS01855 at 355.947 kb on + strand, within MMJJ_RS01855 at 355.948 kb on - strand, within MMJJ_RS01855 at 355.948 kb on - strand, within MMJJ_RS01855 at 355.948 kb on - strand, within MMJJ_RS01855 at 355.949 kb on + strand, within MMJJ_RS01855 at 355.949 kb on + strand, within MMJJ_RS01855 at 355.951 kb on - strand, within MMJJ_RS01855 at 355.952 kb on + strand, within MMJJ_RS01855 at 355.952 kb on + strand, within MMJJ_RS01855 at 355.952 kb on + strand, within MMJJ_RS01855 at 355.953 kb on - strand, within MMJJ_RS01855 at 355.954 kb on + strand, within MMJJ_RS01855 at 355.979 kb on - strand, within MMJJ_RS01855 at 355.980 kb on + strand, within MMJJ_RS01855 at 355.980 kb on + strand, within MMJJ_RS01855 at 356.030 kb on + strand, within MMJJ_RS01855 at 356.039 kb on - strand, within MMJJ_RS01855 at 356.060 kb on - strand, within MMJJ_RS01855 at 356.061 kb on + strand, within MMJJ_RS01855 at 356.063 kb on + strand, within MMJJ_RS01855 at 356.064 kb on - strand, within MMJJ_RS01855 at 356.064 kb on - strand, within MMJJ_RS01855 at 356.068 kb on - strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 353,930 - -1.0 353,966 - +0.3 353,967 + -0.7 353,983 - +0.6 354,001 - -0.9 354,004 + +1.2 354,004 + -4.9 354,046 - +1.0 354,047 + +0.1 354,066 - -0.3 354,066 - +0.5 354,083 - +0.1 354,098 - +0.3 354,133 - MMJJ_RS01845 0.11 -3.8 354,209 - MMJJ_RS01845 0.20 +0.6 354,228 - MMJJ_RS01845 0.23 -0.4 354,228 - MMJJ_RS01845 0.23 -1.0 354,228 - MMJJ_RS01845 0.23 -0.0 354,228 - -1.8 354,229 + MMJJ_RS01845 0.23 -0.6 354,229 + MMJJ_RS01845 0.23 +0.7 354,421 - MMJJ_RS01845 0.47 -4.2 354,422 + MMJJ_RS01845 0.47 -0.3 354,428 - MMJJ_RS01845 0.48 +0.5 354,457 - MMJJ_RS01845 0.51 -2.8 354,477 + MMJJ_RS01845 0.54 -0.2 354,477 + MMJJ_RS01845 0.54 -2.7 354,496 - MMJJ_RS01845 0.56 +0.9 354,496 - MMJJ_RS01845 0.56 +1.3 354,521 - MMJJ_RS01845 0.59 +0.5 354,521 - MMJJ_RS01845 0.59 +0.3 354,522 + MMJJ_RS01845 0.60 +0.2 354,559 - MMJJ_RS01845 0.64 -0.2 354,559 - MMJJ_RS01845 0.64 +0.4 354,560 + MMJJ_RS01845 0.64 -0.2 354,560 + MMJJ_RS01845 0.64 -1.7 354,560 + MMJJ_RS01845 0.64 +0.1 354,560 + MMJJ_RS01845 0.64 +0.4 354,564 - MMJJ_RS01845 0.65 +0.3 354,564 - MMJJ_RS01845 0.65 -1.2 354,565 + MMJJ_RS01845 0.65 -0.7 354,591 - MMJJ_RS01845 0.68 -0.6 354,591 - MMJJ_RS01845 0.68 +0.7 354,591 - MMJJ_RS01845 0.68 +0.0 354,592 + MMJJ_RS01845 0.68 -1.8 354,640 + MMJJ_RS01845 0.74 +0.0 354,679 + MMJJ_RS01845 0.79 -0.3 354,691 - MMJJ_RS01845 0.81 +0.2 354,791 - -0.8 354,791 - -0.0 354,791 - +0.1 354,804 - +0.3 354,813 - +1.0 354,813 - +0.8 354,816 + +0.8 354,819 - +0.5 354,840 + +0.1 354,846 + +0.1 354,877 + -0.7 354,877 + +0.3 354,877 + +0.0 354,878 - +0.3 354,878 - +0.3 354,878 - -0.2 354,879 + -2.2 354,879 + -1.4 354,882 - +1.0 354,883 + +0.3 354,883 + +0.4 354,892 - +0.3 354,896 - +0.2 354,897 + +0.6 354,900 + +0.4 354,915 + -0.3 354,920 - -1.0 354,921 + -0.3 355,006 + MMJJ_RS09265 0.54 +0.1 355,039 - MMJJ_RS09265 0.77 +0.5 355,098 - -0.7 355,119 + -0.7 355,318 - MMJJ_RS01850 0.45 -0.2 355,318 - MMJJ_RS01850 0.45 -0.3 355,318 - MMJJ_RS01850 0.45 -0.7 355,319 + MMJJ_RS01850 0.45 -0.2 355,366 + MMJJ_RS01850 0.54 -0.2 355,447 + MMJJ_RS01850 0.70 +0.0 355,487 - MMJJ_RS01850 0.77 +1.5 355,507 + MMJJ_RS01850 0.81 +0.8 355,529 - MMJJ_RS01850 0.85 -0.8 355,530 + MMJJ_RS01850 0.86 +0.3 355,546 + MMJJ_RS01850 0.89 +0.1 355,546 + MMJJ_RS01850 0.89 +0.2 355,554 - -0.4 355,564 + -1.1 355,579 - -1.0 355,614 - +1.2 355,627 + +0.7 355,651 - +0.4 355,651 - -0.5 355,652 + +1.0 355,673 - MMJJ_RS01855 0.12 -0.9 355,723 - MMJJ_RS01855 0.21 +0.3 355,724 + MMJJ_RS01855 0.22 +0.1 355,779 - MMJJ_RS01855 0.32 +0.5 355,779 - MMJJ_RS01855 0.32 +0.5 355,779 - MMJJ_RS01855 0.32 +0.5 355,779 - MMJJ_RS01855 0.32 +0.2 355,779 - MMJJ_RS01855 0.32 +0.4 355,780 + MMJJ_RS01855 0.32 +0.2 355,780 + MMJJ_RS01855 0.32 -3.3 355,801 - MMJJ_RS01855 0.36 +0.3 355,853 + MMJJ_RS01855 0.45 -0.3 355,862 - MMJJ_RS01855 0.47 -0.6 355,879 + MMJJ_RS01855 0.50 +0.2 355,882 - MMJJ_RS01855 0.51 +0.5 355,901 + MMJJ_RS01855 0.54 +2.1 355,947 + MMJJ_RS01855 0.63 +1.6 355,948 - MMJJ_RS01855 0.63 +0.2 355,948 - MMJJ_RS01855 0.63 +0.3 355,948 - MMJJ_RS01855 0.63 +0.8 355,949 + MMJJ_RS01855 0.63 +0.7 355,949 + MMJJ_RS01855 0.63 +0.9 355,951 - MMJJ_RS01855 0.63 +0.1 355,952 + MMJJ_RS01855 0.64 +0.9 355,952 + MMJJ_RS01855 0.64 +0.1 355,952 + MMJJ_RS01855 0.64 +0.1 355,953 - MMJJ_RS01855 0.64 +0.1 355,954 + MMJJ_RS01855 0.64 -0.6 355,979 - MMJJ_RS01855 0.69 +0.7 355,980 + MMJJ_RS01855 0.69 -0.2 355,980 + MMJJ_RS01855 0.69 +1.1 356,030 + MMJJ_RS01855 0.78 -5.0 356,039 - MMJJ_RS01855 0.80 +0.6 356,060 - MMJJ_RS01855 0.83 +0.5 356,061 + MMJJ_RS01855 0.84 +0.6 356,063 + MMJJ_RS01855 0.84 +0.3 356,064 - MMJJ_RS01855 0.84 +0.3 356,064 - MMJJ_RS01855 0.84 -0.7 356,068 - MMJJ_RS01855 0.85 +0.3 356,069 + MMJJ_RS01855 0.85 -0.2 356,069 + MMJJ_RS01855 0.85 +0.4 356,069 + MMJJ_RS01855 0.85 +0.4 356,069 + MMJJ_RS01855 0.85 +0.8
Or see this region's nucleotide sequence