Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01745 and MMJJ_RS01750 are separated by 111 nucleotides MMJJ_RS01750 and MMJJ_RS09260 are separated by 114 nucleotides MMJJ_RS09260 and MMJJ_RS01755 are separated by 15 nucleotides
MMJJ_RS01745: MMJJ_RS01745 - metal-dependent transcriptional regulator, at 338,240 to 338,645
_RS01745
MMJJ_RS01750: MMJJ_RS01750 - ferrous iron transport protein A, at 338,757 to 338,963
_RS01750
MMJJ_RS09260: MMJJ_RS09260 - hypothetical protein, at 339,078 to 339,215
_RS09260
MMJJ_RS01755: MMJJ_RS01755 - ferrous iron transport protein B, at 339,231 to 341,174
_RS01755
Position (kb) 339
340 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 338.082 kb on + strand at 338.082 kb on + strand at 338.082 kb on + strand at 338.093 kb on + strand at 338.097 kb on - strand at 338.112 kb on - strand at 338.113 kb on + strand at 338.117 kb on + strand at 338.125 kb on - strand at 338.126 kb on + strand at 338.126 kb on + strand at 338.126 kb on + strand at 338.127 kb on - strand at 338.128 kb on + strand at 338.128 kb on + strand at 338.141 kb on + strand at 338.152 kb on + strand at 338.158 kb on - strand at 338.159 kb on + strand at 338.159 kb on + strand at 338.196 kb on - strand at 338.205 kb on - strand at 338.205 kb on - strand at 338.206 kb on + strand at 338.206 kb on + strand at 338.234 kb on - strand at 338.234 kb on - strand at 338.235 kb on + strand at 338.239 kb on + strand at 338.289 kb on + strand, within MMJJ_RS01745 at 338.298 kb on - strand, within MMJJ_RS01745 at 338.301 kb on + strand, within MMJJ_RS01745 at 338.324 kb on - strand, within MMJJ_RS01745 at 338.324 kb on - strand, within MMJJ_RS01745 at 338.337 kb on + strand, within MMJJ_RS01745 at 338.341 kb on - strand, within MMJJ_RS01745 at 338.341 kb on - strand, within MMJJ_RS01745 at 338.342 kb on + strand, within MMJJ_RS01745 at 338.473 kb on - strand, within MMJJ_RS01745 at 338.482 kb on - strand, within MMJJ_RS01745 at 338.586 kb on + strand, within MMJJ_RS01745 at 338.601 kb on - strand, within MMJJ_RS01745 at 338.620 kb on - strand at 338.630 kb on - strand at 338.631 kb on + strand at 338.666 kb on - strand at 338.666 kb on - strand at 338.667 kb on + strand at 338.667 kb on + strand at 338.668 kb on - strand at 338.668 kb on - strand at 338.668 kb on - strand at 338.668 kb on - strand at 338.668 kb on - strand at 338.669 kb on + strand at 338.669 kb on + strand at 338.669 kb on + strand at 338.669 kb on + strand at 338.669 kb on + strand at 338.670 kb on - strand at 338.671 kb on + strand at 338.673 kb on + strand at 338.737 kb on - strand at 338.740 kb on + strand at 338.741 kb on - strand at 338.791 kb on + strand, within MMJJ_RS01750 at 338.792 kb on - strand, within MMJJ_RS01750 at 338.793 kb on + strand, within MMJJ_RS01750 at 338.797 kb on - strand, within MMJJ_RS01750 at 338.869 kb on - strand, within MMJJ_RS01750 at 338.894 kb on + strand, within MMJJ_RS01750 at 338.956 kb on - strand at 338.962 kb on + strand at 338.962 kb on + strand at 338.983 kb on - strand at 338.992 kb on + strand at 338.994 kb on + strand at 338.994 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 339.003 kb on - strand at 339.018 kb on + strand at 339.046 kb on + strand at 339.115 kb on - strand, within MMJJ_RS09260 at 339.285 kb on + strand at 339.388 kb on + strand at 339.538 kb on - strand, within MMJJ_RS01755 at 340.096 kb on - strand, within MMJJ_RS01755 at 340.170 kb on + strand, within MMJJ_RS01755
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 338,082 + -0.7 338,082 + -0.6 338,082 + -4.2 338,093 + -0.6 338,097 - +0.4 338,112 - +1.1 338,113 + -2.2 338,117 + +1.2 338,125 - -0.5 338,126 + +0.1 338,126 + +0.9 338,126 + -4.8 338,127 - +0.3 338,128 + +0.0 338,128 + -0.7 338,141 + +0.1 338,152 + -0.6 338,158 - -0.4 338,159 + -0.3 338,159 + -3.0 338,196 - +0.6 338,205 - +0.7 338,205 - +2.5 338,206 + +0.2 338,206 + -0.2 338,234 - +0.4 338,234 - +0.3 338,235 + +0.4 338,239 + -2.8 338,289 + MMJJ_RS01745 0.12 +0.3 338,298 - MMJJ_RS01745 0.14 +0.3 338,301 + MMJJ_RS01745 0.15 +0.4 338,324 - MMJJ_RS01745 0.21 -0.4 338,324 - MMJJ_RS01745 0.21 -0.6 338,337 + MMJJ_RS01745 0.24 -0.7 338,341 - MMJJ_RS01745 0.25 +0.2 338,341 - MMJJ_RS01745 0.25 +0.2 338,342 + MMJJ_RS01745 0.25 -0.0 338,473 - MMJJ_RS01745 0.57 -0.7 338,482 - MMJJ_RS01745 0.60 +0.7 338,586 + MMJJ_RS01745 0.85 +0.7 338,601 - MMJJ_RS01745 0.89 -1.0 338,620 - +1.2 338,630 - -0.5 338,631 + +0.4 338,666 - +0.8 338,666 - +0.3 338,667 + +0.4 338,667 + +0.0 338,668 - +0.8 338,668 - +0.3 338,668 - +0.4 338,668 - -2.7 338,668 - -0.5 338,669 + -0.7 338,669 + +0.3 338,669 + +1.1 338,669 + +0.4 338,669 + -1.2 338,670 - +0.5 338,671 + +0.7 338,673 + -0.6 338,737 - -1.2 338,740 + -1.8 338,741 - -2.7 338,791 + MMJJ_RS01750 0.16 -3.2 338,792 - MMJJ_RS01750 0.17 -2.8 338,793 + MMJJ_RS01750 0.17 -1.7 338,797 - MMJJ_RS01750 0.19 -1.4 338,869 - MMJJ_RS01750 0.54 -0.8 338,894 + MMJJ_RS01750 0.66 -1.2 338,956 - +0.0 338,962 + -3.5 338,962 + -0.7 338,983 - -1.4 338,992 + +0.0 338,994 + -0.5 338,994 + +0.5 338,999 + +1.1 338,999 + +0.5 338,999 + +0.7 338,999 + -0.9 338,999 + -0.2 338,999 + +0.1 338,999 + +0.2 339,003 - -2.2 339,018 + -1.2 339,046 + -2.0 339,115 - MMJJ_RS09260 0.27 +2.0 339,285 + -0.7 339,388 + -1.2 339,538 - MMJJ_RS01755 0.16 -0.7 340,096 - MMJJ_RS01755 0.44 -1.2 340,170 + MMJJ_RS01755 0.48 -1.8
Or see this region's nucleotide sequence