Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08515 and MMJJ_RS08520 are separated by 114 nucleotides MMJJ_RS08520 and MMJJ_RS08525 are separated by 41 nucleotides MMJJ_RS08525 and MMJJ_RS08530 are separated by 42 nucleotides
MMJJ_RS08515: MMJJ_RS08515 - secondary thiamine-phosphate synthase enzyme YjbQ, at 1,567,020 to 1,567,418
_RS08515
MMJJ_RS08520: MMJJ_RS08520 - peptidylprolyl isomerase, at 1,567,533 to 1,568,021
_RS08520
MMJJ_RS08525: MMJJ_RS08525 - peptidylprolyl isomerase, at 1,568,063 to 1,568,665
_RS08525
MMJJ_RS08530: MMJJ_RS08530 - RNA-binding transcriptional accessory protein, at 1,568,708 to 1,570,849
_RS08530
Position (kb)
1568
1569 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1567.088 kb on + strand, within MMJJ_RS08515 at 1567.148 kb on - strand, within MMJJ_RS08515 at 1567.165 kb on - strand, within MMJJ_RS08515 at 1567.165 kb on - strand, within MMJJ_RS08515 at 1567.166 kb on + strand, within MMJJ_RS08515 at 1567.252 kb on + strand, within MMJJ_RS08515 at 1567.252 kb on + strand, within MMJJ_RS08515 at 1567.315 kb on - strand, within MMJJ_RS08515 at 1567.316 kb on + strand, within MMJJ_RS08515 at 1567.421 kb on - strand at 1567.421 kb on - strand at 1567.421 kb on - strand at 1567.422 kb on + strand at 1567.442 kb on + strand at 1567.449 kb on + strand at 1567.454 kb on + strand at 1567.573 kb on - strand at 1567.579 kb on - strand at 1567.579 kb on - strand at 1567.666 kb on - strand, within MMJJ_RS08520 at 1567.808 kb on + strand, within MMJJ_RS08520 at 1567.835 kb on + strand, within MMJJ_RS08520 at 1567.966 kb on + strand, within MMJJ_RS08520 at 1568.010 kb on + strand at 1568.037 kb on - strand at 1568.038 kb on + strand at 1568.089 kb on + strand at 1568.132 kb on + strand, within MMJJ_RS08525 at 1568.172 kb on - strand, within MMJJ_RS08525 at 1568.173 kb on + strand, within MMJJ_RS08525 at 1568.178 kb on + strand, within MMJJ_RS08525 at 1568.310 kb on - strand at 1568.311 kb on + strand, within MMJJ_RS08525 at 1568.448 kb on - strand, within MMJJ_RS08525 at 1568.449 kb on + strand, within MMJJ_RS08525 at 1568.496 kb on + strand, within MMJJ_RS08525 at 1568.542 kb on - strand, within MMJJ_RS08525 at 1568.600 kb on + strand, within MMJJ_RS08525 at 1568.634 kb on - strand at 1568.635 kb on + strand at 1568.650 kb on - strand at 1568.680 kb on + strand at 1568.680 kb on + strand at 1568.710 kb on + strand at 1568.765 kb on - strand at 1568.766 kb on + strand at 1568.771 kb on + strand at 1568.798 kb on - strand at 1568.816 kb on - strand at 1568.816 kb on - strand at 1568.816 kb on - strand at 1568.817 kb on + strand at 1568.879 kb on + strand at 1568.899 kb on - strand at 1568.900 kb on + strand at 1568.919 kb on + strand at 1568.936 kb on - strand, within MMJJ_RS08530 at 1569.026 kb on - strand, within MMJJ_RS08530 at 1569.036 kb on + strand, within MMJJ_RS08530 at 1569.148 kb on - strand, within MMJJ_RS08530 at 1569.171 kb on - strand, within MMJJ_RS08530 at 1569.171 kb on - strand, within MMJJ_RS08530 at 1569.172 kb on + strand, within MMJJ_RS08530 at 1569.272 kb on - strand, within MMJJ_RS08530 at 1569.273 kb on + strand, within MMJJ_RS08530 at 1569.279 kb on + strand, within MMJJ_RS08530 at 1569.279 kb on + strand, within MMJJ_RS08530 at 1569.322 kb on + strand, within MMJJ_RS08530 at 1569.326 kb on - strand, within MMJJ_RS08530 at 1569.327 kb on + strand, within MMJJ_RS08530 at 1569.327 kb on + strand, within MMJJ_RS08530 at 1569.327 kb on + strand, within MMJJ_RS08530 at 1569.366 kb on - strand, within MMJJ_RS08530 at 1569.366 kb on - strand, within MMJJ_RS08530 at 1569.366 kb on - strand, within MMJJ_RS08530 at 1569.396 kb on + strand, within MMJJ_RS08530 at 1569.558 kb on + strand, within MMJJ_RS08530 at 1569.569 kb on + strand, within MMJJ_RS08530 at 1569.569 kb on + strand, within MMJJ_RS08530 at 1569.622 kb on + strand, within MMJJ_RS08530 at 1569.644 kb on - strand, within MMJJ_RS08530 at 1569.650 kb on - strand, within MMJJ_RS08530 at 1569.651 kb on + strand, within MMJJ_RS08530 at 1569.651 kb on + strand, within MMJJ_RS08530 at 1569.651 kb on + strand, within MMJJ_RS08530 at 1569.655 kb on + strand, within MMJJ_RS08530 at 1569.657 kb on - strand, within MMJJ_RS08530 at 1569.657 kb on - strand, within MMJJ_RS08530
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 1,567,088 + MMJJ_RS08515 0.17 -2.5 1,567,148 - MMJJ_RS08515 0.32 +0.4 1,567,165 - MMJJ_RS08515 0.36 -5.1 1,567,165 - MMJJ_RS08515 0.36 +0.5 1,567,166 + MMJJ_RS08515 0.37 +0.6 1,567,252 + MMJJ_RS08515 0.58 +0.2 1,567,252 + MMJJ_RS08515 0.58 +0.6 1,567,315 - MMJJ_RS08515 0.74 -3.3 1,567,316 + MMJJ_RS08515 0.74 +0.5 1,567,421 - -3.2 1,567,421 - +0.3 1,567,421 - +0.2 1,567,422 + -0.6 1,567,442 + +0.7 1,567,449 + +0.2 1,567,454 + -1.3 1,567,573 - +0.8 1,567,579 - -0.3 1,567,579 - -0.2 1,567,666 - MMJJ_RS08520 0.27 -1.3 1,567,808 + MMJJ_RS08520 0.56 +0.4 1,567,835 + MMJJ_RS08520 0.62 +0.5 1,567,966 + MMJJ_RS08520 0.89 -0.4 1,568,010 + -1.0 1,568,037 - +0.8 1,568,038 + +2.8 1,568,089 + +0.3 1,568,132 + MMJJ_RS08525 0.11 +0.1 1,568,172 - MMJJ_RS08525 0.18 +0.1 1,568,173 + MMJJ_RS08525 0.18 +0.6 1,568,178 + MMJJ_RS08525 0.19 -0.5 1,568,310 - -0.1 1,568,311 + MMJJ_RS08525 0.41 +0.4 1,568,448 - MMJJ_RS08525 0.64 -5.1 1,568,449 + MMJJ_RS08525 0.64 +0.9 1,568,496 + MMJJ_RS08525 0.72 +0.9 1,568,542 - MMJJ_RS08525 0.79 +0.5 1,568,600 + MMJJ_RS08525 0.89 +0.6 1,568,634 - -0.5 1,568,635 + -0.0 1,568,650 - +1.1 1,568,680 + -0.2 1,568,680 + +0.9 1,568,710 + -0.8 1,568,765 - +0.4 1,568,766 + -3.2 1,568,771 + +2.3 1,568,798 - -1.7 1,568,816 - +0.4 1,568,816 - +0.6 1,568,816 - +0.1 1,568,817 + +0.0 1,568,879 + -2.9 1,568,899 - +0.3 1,568,900 + -2.1 1,568,919 + -0.4 1,568,936 - MMJJ_RS08530 0.11 -0.1 1,569,026 - MMJJ_RS08530 0.15 +0.5 1,569,036 + MMJJ_RS08530 0.15 -1.7 1,569,148 - MMJJ_RS08530 0.21 +0.3 1,569,171 - MMJJ_RS08530 0.22 -0.0 1,569,171 - MMJJ_RS08530 0.22 +0.4 1,569,172 + MMJJ_RS08530 0.22 -2.4 1,569,272 - MMJJ_RS08530 0.26 -0.2 1,569,273 + MMJJ_RS08530 0.26 +0.9 1,569,279 + MMJJ_RS08530 0.27 +0.9 1,569,279 + MMJJ_RS08530 0.27 +0.5 1,569,322 + MMJJ_RS08530 0.29 +0.4 1,569,326 - MMJJ_RS08530 0.29 -0.3 1,569,327 + MMJJ_RS08530 0.29 -0.4 1,569,327 + MMJJ_RS08530 0.29 -1.2 1,569,327 + MMJJ_RS08530 0.29 -1.4 1,569,366 - MMJJ_RS08530 0.31 +0.3 1,569,366 - MMJJ_RS08530 0.31 +2.3 1,569,366 - MMJJ_RS08530 0.31 +0.5 1,569,396 + MMJJ_RS08530 0.32 +0.9 1,569,558 + MMJJ_RS08530 0.40 +0.4 1,569,569 + MMJJ_RS08530 0.40 +0.7 1,569,569 + MMJJ_RS08530 0.40 +0.4 1,569,622 + MMJJ_RS08530 0.43 +1.5 1,569,644 - MMJJ_RS08530 0.44 -0.0 1,569,650 - MMJJ_RS08530 0.44 +0.9 1,569,651 + MMJJ_RS08530 0.44 +0.6 1,569,651 + MMJJ_RS08530 0.44 +0.4 1,569,651 + MMJJ_RS08530 0.44 +0.1 1,569,655 + MMJJ_RS08530 0.44 -0.3 1,569,657 - MMJJ_RS08530 0.44 +0.6 1,569,657 - MMJJ_RS08530 0.44 +0.8
Or see this region's nucleotide sequence