Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08375 and MMJJ_RS08380 are separated by 22 nucleotides MMJJ_RS08380 and MMJJ_RS08385 are separated by 20 nucleotides MMJJ_RS08385 and MMJJ_RS08390 are separated by 13 nucleotides MMJJ_RS08390 and MMJJ_RS08395 are separated by 33 nucleotides MMJJ_RS08395 and MMJJ_RS08400 are separated by 12 nucleotides
MMJJ_RS08375: MMJJ_RS08375 - ferritin, at 1,543,377 to 1,543,892
_RS08375
MMJJ_RS08380: MMJJ_RS08380 - hypothetical protein, at 1,543,915 to 1,544,181
_RS08380
MMJJ_RS08385: MMJJ_RS08385 - class II SORL domain-containing protein, at 1,544,202 to 1,544,558
_RS08385
MMJJ_RS08390: MMJJ_RS08390 - carboxymuconolactone decarboxylase family protein, at 1,544,572 to 1,544,886
_RS08390
MMJJ_RS08395: MMJJ_RS08395 - CoB--CoM heterodisulfide reductase subunit B, at 1,544,920 to 1,545,825
_RS08395
MMJJ_RS08400: MMJJ_RS08400 - CoB--CoM heterodisulfide reductase subunit C, at 1,545,838 to 1,546,416
_RS08400
Position (kb)
1544
1545 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1543.609 kb on - strand, within MMJJ_RS08375 at 1543.610 kb on + strand, within MMJJ_RS08375 at 1543.610 kb on + strand, within MMJJ_RS08375 at 1543.623 kb on + strand, within MMJJ_RS08375 at 1543.644 kb on - strand, within MMJJ_RS08375 at 1543.644 kb on - strand, within MMJJ_RS08375 at 1543.644 kb on - strand, within MMJJ_RS08375 at 1543.645 kb on + strand, within MMJJ_RS08375 at 1543.645 kb on + strand, within MMJJ_RS08375 at 1543.646 kb on - strand, within MMJJ_RS08375 at 1543.647 kb on + strand, within MMJJ_RS08375 at 1543.650 kb on + strand, within MMJJ_RS08375 at 1543.713 kb on - strand, within MMJJ_RS08375 at 1543.713 kb on - strand, within MMJJ_RS08375 at 1543.713 kb on - strand, within MMJJ_RS08375 at 1543.714 kb on + strand, within MMJJ_RS08375 at 1543.714 kb on + strand, within MMJJ_RS08375 at 1543.714 kb on + strand, within MMJJ_RS08375 at 1543.717 kb on - strand, within MMJJ_RS08375 at 1543.717 kb on - strand, within MMJJ_RS08375 at 1543.718 kb on + strand, within MMJJ_RS08375 at 1543.807 kb on - strand, within MMJJ_RS08375 at 1543.808 kb on + strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.827 kb on - strand, within MMJJ_RS08375 at 1543.828 kb on + strand, within MMJJ_RS08375 at 1543.828 kb on + strand, within MMJJ_RS08375 at 1543.828 kb on + strand, within MMJJ_RS08375 at 1543.831 kb on - strand, within MMJJ_RS08375 at 1543.831 kb on - strand, within MMJJ_RS08375 at 1543.831 kb on - strand, within MMJJ_RS08375 at 1543.831 kb on - strand, within MMJJ_RS08375 at 1543.832 kb on + strand, within MMJJ_RS08375 at 1543.840 kb on - strand, within MMJJ_RS08375 at 1543.841 kb on + strand at 1543.866 kb on - strand at 1543.867 kb on + strand at 1543.878 kb on - strand at 1543.879 kb on + strand at 1543.879 kb on + strand at 1543.879 kb on + strand at 1543.879 kb on + strand at 1543.883 kb on - strand at 1543.892 kb on - strand at 1543.909 kb on - strand at 1543.909 kb on - strand at 1543.910 kb on + strand at 1543.920 kb on - strand at 1543.921 kb on + strand at 1543.921 kb on + strand at 1543.936 kb on + strand at 1543.990 kb on - strand, within MMJJ_RS08380 at 1544.018 kb on + strand at 1544.018 kb on + strand, within MMJJ_RS08380 at 1544.018 kb on + strand, within MMJJ_RS08380 at 1544.090 kb on + strand, within MMJJ_RS08380 at 1544.313 kb on + strand, within MMJJ_RS08385 at 1544.317 kb on + strand, within MMJJ_RS08385 at 1544.320 kb on + strand, within MMJJ_RS08385 at 1544.347 kb on + strand, within MMJJ_RS08385 at 1544.358 kb on - strand, within MMJJ_RS08385 at 1544.376 kb on - strand, within MMJJ_RS08385 at 1544.377 kb on + strand, within MMJJ_RS08385 at 1544.382 kb on - strand, within MMJJ_RS08385 at 1544.382 kb on - strand, within MMJJ_RS08385 at 1544.382 kb on - strand, within MMJJ_RS08385 at 1544.383 kb on + strand, within MMJJ_RS08385 at 1544.391 kb on + strand, within MMJJ_RS08385 at 1544.428 kb on + strand, within MMJJ_RS08385 at 1544.448 kb on - strand, within MMJJ_RS08385 at 1544.449 kb on + strand, within MMJJ_RS08385 at 1544.491 kb on + strand, within MMJJ_RS08385 at 1544.547 kb on - strand at 1544.559 kb on - strand at 1544.559 kb on - strand at 1544.560 kb on + strand at 1544.560 kb on + strand at 1544.587 kb on - strand at 1544.587 kb on - strand at 1544.588 kb on + strand at 1544.604 kb on - strand, within MMJJ_RS08390 at 1544.627 kb on + strand, within MMJJ_RS08390 at 1544.629 kb on - strand, within MMJJ_RS08390 at 1544.633 kb on + strand, within MMJJ_RS08390 at 1544.790 kb on + strand, within MMJJ_RS08390 at 1544.823 kb on + strand, within MMJJ_RS08390 at 1544.916 kb on - strand at 1544.921 kb on - strand at 1544.921 kb on - strand at 1544.922 kb on + strand at 1544.922 kb on + strand at 1544.922 kb on + strand at 1544.928 kb on - strand at 1544.932 kb on - strand at 1544.932 kb on - strand at 1545.003 kb on - strand at 1545.032 kb on - strand, within MMJJ_RS08395 at 1545.121 kb on - strand, within MMJJ_RS08395 at 1545.212 kb on + strand, within MMJJ_RS08395 at 1545.237 kb on - strand, within MMJJ_RS08395 at 1545.238 kb on + strand, within MMJJ_RS08395 at 1545.249 kb on - strand, within MMJJ_RS08395 at 1545.250 kb on + strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.251 kb on - strand, within MMJJ_RS08395 at 1545.252 kb on + strand, within MMJJ_RS08395 at 1545.252 kb on + strand, within MMJJ_RS08395 at 1545.252 kb on + strand, within MMJJ_RS08395 at 1545.252 kb on + strand, within MMJJ_RS08395 at 1545.254 kb on - strand, within MMJJ_RS08395 at 1545.254 kb on - strand, within MMJJ_RS08395 at 1545.254 kb on - strand, within MMJJ_RS08395 at 1545.254 kb on - strand, within MMJJ_RS08395 at 1545.254 kb on - strand, within MMJJ_RS08395 at 1545.255 kb on + strand, within MMJJ_RS08395 at 1545.255 kb on + strand, within MMJJ_RS08395 at 1545.257 kb on + strand, within MMJJ_RS08395 at 1545.321 kb on - strand, within MMJJ_RS08395 at 1545.322 kb on + strand, within MMJJ_RS08395 at 1545.329 kb on + strand, within MMJJ_RS08395 at 1545.337 kb on + strand, within MMJJ_RS08395 at 1545.337 kb on + strand, within MMJJ_RS08395 at 1545.343 kb on - strand, within MMJJ_RS08395 at 1545.343 kb on - strand, within MMJJ_RS08395 at 1545.344 kb on + strand, within MMJJ_RS08395 at 1545.344 kb on + strand, within MMJJ_RS08395 at 1545.347 kb on - strand, within MMJJ_RS08395 at 1545.359 kb on - strand, within MMJJ_RS08395 at 1545.431 kb on - strand, within MMJJ_RS08395 at 1545.432 kb on + strand, within MMJJ_RS08395 at 1545.436 kb on - strand, within MMJJ_RS08395 at 1545.436 kb on - strand, within MMJJ_RS08395 at 1545.436 kb on - strand, within MMJJ_RS08395 at 1545.472 kb on + strand, within MMJJ_RS08395 at 1545.473 kb on - strand, within MMJJ_RS08395 at 1545.473 kb on - strand, within MMJJ_RS08395 at 1545.473 kb on - strand, within MMJJ_RS08395 at 1545.473 kb on - strand, within MMJJ_RS08395 at 1545.505 kb on - strand, within MMJJ_RS08395 at 1545.505 kb on - strand, within MMJJ_RS08395 at 1545.505 kb on - strand, within MMJJ_RS08395 at 1545.510 kb on - strand, within MMJJ_RS08395 at 1545.510 kb on - strand, within MMJJ_RS08395 at 1545.510 kb on - strand, within MMJJ_RS08395 at 1545.511 kb on + strand, within MMJJ_RS08395 at 1545.567 kb on + strand, within MMJJ_RS08395 at 1545.576 kb on - strand, within MMJJ_RS08395 at 1545.577 kb on + strand, within MMJJ_RS08395 at 1545.580 kb on - strand, within MMJJ_RS08395 at 1545.580 kb on - strand, within MMJJ_RS08395 at 1545.617 kb on + strand, within MMJJ_RS08395 at 1545.617 kb on + strand, within MMJJ_RS08395 at 1545.621 kb on - strand, within MMJJ_RS08395 at 1545.640 kb on + strand, within MMJJ_RS08395 at 1545.640 kb on + strand, within MMJJ_RS08395 at 1545.640 kb on + strand, within MMJJ_RS08395 at 1545.743 kb on - strand at 1545.772 kb on + strand at 1545.804 kb on + strand at 1545.828 kb on - strand at 1545.839 kb on - strand at 1545.862 kb on - strand at 1545.879 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 1,543,609 - MMJJ_RS08375 0.45 +1.2 1,543,610 + MMJJ_RS08375 0.45 -0.0 1,543,610 + MMJJ_RS08375 0.45 +0.9 1,543,623 + MMJJ_RS08375 0.48 +0.1 1,543,644 - MMJJ_RS08375 0.52 +0.2 1,543,644 - MMJJ_RS08375 0.52 +1.4 1,543,644 - MMJJ_RS08375 0.52 +0.7 1,543,645 + MMJJ_RS08375 0.52 -1.5 1,543,645 + MMJJ_RS08375 0.52 +1.4 1,543,646 - MMJJ_RS08375 0.52 +0.6 1,543,647 + MMJJ_RS08375 0.52 +0.3 1,543,650 + MMJJ_RS08375 0.53 -0.1 1,543,713 - MMJJ_RS08375 0.65 +0.7 1,543,713 - MMJJ_RS08375 0.65 +0.7 1,543,713 - MMJJ_RS08375 0.65 +0.4 1,543,714 + MMJJ_RS08375 0.65 +0.7 1,543,714 + MMJJ_RS08375 0.65 +0.8 1,543,714 + MMJJ_RS08375 0.65 -2.8 1,543,717 - MMJJ_RS08375 0.66 +2.1 1,543,717 - MMJJ_RS08375 0.66 +0.0 1,543,718 + MMJJ_RS08375 0.66 +2.8 1,543,807 - MMJJ_RS08375 0.83 +1.5 1,543,808 + MMJJ_RS08375 0.84 +0.6 1,543,827 - MMJJ_RS08375 0.87 -3.8 1,543,827 - MMJJ_RS08375 0.87 -0.4 1,543,827 - MMJJ_RS08375 0.87 +0.5 1,543,827 - MMJJ_RS08375 0.87 -0.7 1,543,827 - MMJJ_RS08375 0.87 -0.4 1,543,827 - MMJJ_RS08375 0.87 +1.1 1,543,827 - MMJJ_RS08375 0.87 -0.7 1,543,828 + MMJJ_RS08375 0.87 -4.6 1,543,828 + MMJJ_RS08375 0.87 +0.6 1,543,828 + MMJJ_RS08375 0.87 -0.1 1,543,831 - MMJJ_RS08375 0.88 -0.1 1,543,831 - MMJJ_RS08375 0.88 -0.4 1,543,831 - MMJJ_RS08375 0.88 +0.3 1,543,831 - MMJJ_RS08375 0.88 +0.2 1,543,832 + MMJJ_RS08375 0.88 -1.5 1,543,840 - MMJJ_RS08375 0.90 -0.0 1,543,841 + +1.3 1,543,866 - +0.7 1,543,867 + -0.0 1,543,878 - +1.2 1,543,879 + +0.1 1,543,879 + +0.2 1,543,879 + -0.0 1,543,879 + +0.4 1,543,883 - +1.3 1,543,892 - -0.4 1,543,909 - +1.8 1,543,909 - -0.2 1,543,910 + -0.0 1,543,920 - -0.2 1,543,921 + -0.5 1,543,921 + +1.6 1,543,936 + +0.9 1,543,990 - MMJJ_RS08380 0.28 +0.1 1,544,018 + -1.0 1,544,018 + MMJJ_RS08380 0.39 +0.0 1,544,018 + MMJJ_RS08380 0.39 +0.8 1,544,090 + MMJJ_RS08380 0.66 -1.7 1,544,313 + MMJJ_RS08385 0.31 -0.2 1,544,317 + MMJJ_RS08385 0.32 +1.6 1,544,320 + MMJJ_RS08385 0.33 +0.2 1,544,347 + MMJJ_RS08385 0.41 +0.1 1,544,358 - MMJJ_RS08385 0.44 +0.4 1,544,376 - MMJJ_RS08385 0.49 +0.8 1,544,377 + MMJJ_RS08385 0.49 +0.2 1,544,382 - MMJJ_RS08385 0.50 -1.2 1,544,382 - MMJJ_RS08385 0.50 -0.1 1,544,382 - MMJJ_RS08385 0.50 +0.7 1,544,383 + MMJJ_RS08385 0.51 +0.2 1,544,391 + MMJJ_RS08385 0.53 -0.1 1,544,428 + MMJJ_RS08385 0.63 +0.7 1,544,448 - MMJJ_RS08385 0.69 +0.4 1,544,449 + MMJJ_RS08385 0.69 -0.4 1,544,491 + MMJJ_RS08385 0.81 +1.1 1,544,547 - -1.1 1,544,559 - +0.8 1,544,559 - -0.3 1,544,560 + +0.2 1,544,560 + -0.2 1,544,587 - -0.1 1,544,587 - +0.1 1,544,588 + -0.3 1,544,604 - MMJJ_RS08390 0.10 -0.3 1,544,627 + MMJJ_RS08390 0.17 +2.6 1,544,629 - MMJJ_RS08390 0.18 +0.2 1,544,633 + MMJJ_RS08390 0.19 +0.3 1,544,790 + MMJJ_RS08390 0.69 -1.2 1,544,823 + MMJJ_RS08390 0.80 +0.5 1,544,916 - +0.3 1,544,921 - +0.2 1,544,921 - -1.5 1,544,922 + +0.2 1,544,922 + +0.0 1,544,922 + +0.1 1,544,928 - +0.7 1,544,932 - +0.4 1,544,932 - +0.3 1,545,003 - +0.8 1,545,032 - MMJJ_RS08395 0.12 +0.1 1,545,121 - MMJJ_RS08395 0.22 +0.2 1,545,212 + MMJJ_RS08395 0.32 +0.4 1,545,237 - MMJJ_RS08395 0.35 +0.4 1,545,238 + MMJJ_RS08395 0.35 -0.0 1,545,249 - MMJJ_RS08395 0.36 +1.2 1,545,250 + MMJJ_RS08395 0.36 +1.7 1,545,251 - MMJJ_RS08395 0.37 -0.3 1,545,251 - MMJJ_RS08395 0.37 -0.3 1,545,251 - MMJJ_RS08395 0.37 +0.6 1,545,251 - MMJJ_RS08395 0.37 -0.0 1,545,251 - MMJJ_RS08395 0.37 +2.0 1,545,251 - MMJJ_RS08395 0.37 -0.0 1,545,252 + MMJJ_RS08395 0.37 -0.8 1,545,252 + MMJJ_RS08395 0.37 -2.8 1,545,252 + MMJJ_RS08395 0.37 -0.2 1,545,252 + MMJJ_RS08395 0.37 +0.5 1,545,254 - MMJJ_RS08395 0.37 -2.8 1,545,254 - MMJJ_RS08395 0.37 -0.0 1,545,254 - MMJJ_RS08395 0.37 +0.3 1,545,254 - MMJJ_RS08395 0.37 -0.0 1,545,254 - MMJJ_RS08395 0.37 -1.8 1,545,255 + MMJJ_RS08395 0.37 -0.5 1,545,255 + MMJJ_RS08395 0.37 +0.4 1,545,257 + MMJJ_RS08395 0.37 +1.2 1,545,321 - MMJJ_RS08395 0.44 +0.5 1,545,322 + MMJJ_RS08395 0.44 -2.0 1,545,329 + MMJJ_RS08395 0.45 -1.0 1,545,337 + MMJJ_RS08395 0.46 +0.8 1,545,337 + MMJJ_RS08395 0.46 +0.2 1,545,343 - MMJJ_RS08395 0.47 +0.9 1,545,343 - MMJJ_RS08395 0.47 -0.6 1,545,344 + MMJJ_RS08395 0.47 -0.1 1,545,344 + MMJJ_RS08395 0.47 +0.8 1,545,347 - MMJJ_RS08395 0.47 +0.7 1,545,359 - MMJJ_RS08395 0.48 -1.8 1,545,431 - MMJJ_RS08395 0.56 -0.0 1,545,432 + MMJJ_RS08395 0.57 -0.0 1,545,436 - MMJJ_RS08395 0.57 +0.5 1,545,436 - MMJJ_RS08395 0.57 +0.1 1,545,436 - MMJJ_RS08395 0.57 -1.9 1,545,472 + MMJJ_RS08395 0.61 -0.0 1,545,473 - MMJJ_RS08395 0.61 -2.4 1,545,473 - MMJJ_RS08395 0.61 +0.5 1,545,473 - MMJJ_RS08395 0.61 -1.1 1,545,473 - MMJJ_RS08395 0.61 +1.9 1,545,505 - MMJJ_RS08395 0.65 -0.2 1,545,505 - MMJJ_RS08395 0.65 +0.3 1,545,505 - MMJJ_RS08395 0.65 -0.2 1,545,510 - MMJJ_RS08395 0.65 -3.5 1,545,510 - MMJJ_RS08395 0.65 -1.5 1,545,510 - MMJJ_RS08395 0.65 +0.4 1,545,511 + MMJJ_RS08395 0.65 +0.2 1,545,567 + MMJJ_RS08395 0.71 +0.1 1,545,576 - MMJJ_RS08395 0.72 +0.4 1,545,577 + MMJJ_RS08395 0.73 +0.0 1,545,580 - MMJJ_RS08395 0.73 -0.2 1,545,580 - MMJJ_RS08395 0.73 +0.6 1,545,617 + MMJJ_RS08395 0.77 -0.9 1,545,617 + MMJJ_RS08395 0.77 -0.2 1,545,621 - MMJJ_RS08395 0.77 +1.0 1,545,640 + MMJJ_RS08395 0.79 -0.3 1,545,640 + MMJJ_RS08395 0.79 -0.1 1,545,640 + MMJJ_RS08395 0.79 -1.7 1,545,743 - +0.2 1,545,772 + +0.1 1,545,804 + +0.8 1,545,828 - -3.4 1,545,839 - +0.0 1,545,862 - +1.4 1,545,879 - +0.7
Or see this region's nucleotide sequence