Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08160 and MMJJ_RS08165 are separated by 9 nucleotides MMJJ_RS08165 and MMJJ_RS08170 are separated by 252 nucleotides MMJJ_RS08170 and MMJJ_RS08175 are separated by 136 nucleotides
MMJJ_RS08160: MMJJ_RS08160 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,498,932 to 1,499,345
_RS08160
MMJJ_RS08165: MMJJ_RS08165 - thermonuclease family protein, at 1,499,355 to 1,499,912
_RS08165
MMJJ_RS08170: MMJJ_RS08170 - diaminopimelate decarboxylase, at 1,500,165 to 1,501,475
_RS08170
MMJJ_RS08175: MMJJ_RS08175 - winged helix-turn-helix transcriptional regulator, at 1,501,612 to 1,502,466
_RS08175
Position (kb)
1500
1501
1502 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1499.176 kb on - strand, within MMJJ_RS08160 at 1499.192 kb on - strand, within MMJJ_RS08160 at 1499.229 kb on - strand, within MMJJ_RS08160 at 1499.236 kb on - strand, within MMJJ_RS08160 at 1499.237 kb on + strand, within MMJJ_RS08160 at 1499.241 kb on + strand, within MMJJ_RS08160 at 1499.241 kb on + strand, within MMJJ_RS08160 at 1499.261 kb on - strand, within MMJJ_RS08160 at 1499.268 kb on - strand, within MMJJ_RS08160 at 1499.268 kb on - strand, within MMJJ_RS08160 at 1499.268 kb on - strand, within MMJJ_RS08160 at 1499.268 kb on - strand, within MMJJ_RS08160 at 1499.268 kb on - strand, within MMJJ_RS08160 at 1499.269 kb on + strand, within MMJJ_RS08160 at 1499.269 kb on + strand, within MMJJ_RS08160 at 1499.270 kb on - strand, within MMJJ_RS08160 at 1499.270 kb on - strand, within MMJJ_RS08160 at 1499.271 kb on + strand, within MMJJ_RS08160 at 1499.271 kb on + strand, within MMJJ_RS08160 at 1499.312 kb on - strand at 1499.313 kb on + strand at 1499.315 kb on + strand at 1499.408 kb on - strand at 1499.409 kb on + strand at 1499.431 kb on + strand, within MMJJ_RS08165 at 1499.450 kb on + strand, within MMJJ_RS08165 at 1499.533 kb on + strand, within MMJJ_RS08165 at 1499.534 kb on - strand, within MMJJ_RS08165 at 1499.535 kb on + strand, within MMJJ_RS08165 at 1499.538 kb on + strand, within MMJJ_RS08165 at 1499.549 kb on + strand, within MMJJ_RS08165 at 1499.599 kb on + strand, within MMJJ_RS08165 at 1499.634 kb on - strand, within MMJJ_RS08165 at 1499.670 kb on + strand, within MMJJ_RS08165 at 1499.758 kb on + strand, within MMJJ_RS08165 at 1499.758 kb on + strand, within MMJJ_RS08165 at 1499.760 kb on + strand, within MMJJ_RS08165 at 1499.818 kb on - strand, within MMJJ_RS08165 at 1499.819 kb on + strand, within MMJJ_RS08165 at 1499.822 kb on - strand, within MMJJ_RS08165 at 1499.822 kb on - strand, within MMJJ_RS08165 at 1499.823 kb on + strand, within MMJJ_RS08165 at 1499.902 kb on + strand at 1499.902 kb on + strand at 1499.923 kb on - strand at 1499.975 kb on - strand at 1499.976 kb on + strand at 1499.988 kb on + strand at 1500.009 kb on + strand at 1500.011 kb on + strand at 1500.028 kb on - strand at 1500.028 kb on - strand at 1500.039 kb on - strand at 1500.041 kb on - strand at 1500.041 kb on - strand at 1500.046 kb on - strand at 1500.046 kb on - strand at 1500.047 kb on + strand at 1500.050 kb on + strand at 1500.058 kb on - strand at 1500.059 kb on + strand at 1500.062 kb on + strand at 1500.064 kb on - strand at 1500.065 kb on + strand at 1500.065 kb on + strand at 1500.065 kb on + strand at 1500.069 kb on + strand at 1500.069 kb on + strand at 1500.086 kb on + strand at 1500.088 kb on - strand at 1500.088 kb on - strand at 1500.088 kb on - strand at 1500.088 kb on - strand at 1500.088 kb on - strand at 1500.088 kb on - strand at 1500.093 kb on + strand at 1500.093 kb on + strand at 1500.093 kb on + strand at 1500.107 kb on - strand at 1500.107 kb on - strand at 1500.130 kb on + strand at 1500.162 kb on + strand at 1500.167 kb on + strand at 1500.255 kb on + strand at 1500.443 kb on - strand, within MMJJ_RS08170 at 1500.473 kb on + strand at 1500.768 kb on - strand, within MMJJ_RS08170 at 1501.487 kb on + strand at 1501.522 kb on + strand at 1501.526 kb on + strand at 1501.538 kb on - strand at 1501.543 kb on - strand at 1501.543 kb on - strand at 1501.543 kb on - strand at 1501.543 kb on - strand at 1501.544 kb on + strand at 1501.544 kb on + strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.545 kb on - strand at 1501.546 kb on + strand at 1501.546 kb on + strand at 1501.546 kb on + strand at 1501.546 kb on + strand at 1501.546 kb on + strand at 1501.546 kb on + strand at 1501.547 kb on - strand at 1501.547 kb on - strand at 1501.547 kb on - strand at 1501.547 kb on - strand at 1501.548 kb on + strand at 1501.548 kb on + strand at 1501.548 kb on + strand at 1501.560 kb on + strand at 1501.563 kb on - strand at 1501.564 kb on + strand at 1501.567 kb on + strand at 1501.603 kb on + strand at 1501.609 kb on - strand at 1501.613 kb on - strand at 1501.631 kb on - strand at 1501.631 kb on - strand at 1501.631 kb on - strand at 1501.632 kb on + strand at 1501.691 kb on + strand at 1501.704 kb on - strand, within MMJJ_RS08175 at 1501.761 kb on - strand, within MMJJ_RS08175 at 1501.762 kb on + strand, within MMJJ_RS08175 at 1501.838 kb on + strand, within MMJJ_RS08175 at 1501.879 kb on + strand, within MMJJ_RS08175 at 1501.910 kb on + strand, within MMJJ_RS08175 at 1501.966 kb on - strand, within MMJJ_RS08175 at 1502.014 kb on + strand, within MMJJ_RS08175 at 1502.061 kb on + strand, within MMJJ_RS08175 at 1502.061 kb on + strand, within MMJJ_RS08175 at 1502.084 kb on + strand, within MMJJ_RS08175 at 1502.084 kb on + strand, within MMJJ_RS08175 at 1502.092 kb on - strand, within MMJJ_RS08175 at 1502.093 kb on + strand, within MMJJ_RS08175 at 1502.121 kb on + strand, within MMJJ_RS08175 at 1502.131 kb on - strand, within MMJJ_RS08175 at 1502.134 kb on + strand, within MMJJ_RS08175 at 1502.142 kb on - strand, within MMJJ_RS08175 at 1502.148 kb on - strand, within MMJJ_RS08175 at 1502.185 kb on + strand, within MMJJ_RS08175 at 1502.188 kb on - strand, within MMJJ_RS08175 at 1502.190 kb on - strand, within MMJJ_RS08175 at 1502.199 kb on - strand, within MMJJ_RS08175 at 1502.205 kb on - strand, within MMJJ_RS08175 at 1502.205 kb on - strand, within MMJJ_RS08175 at 1502.265 kb on + strand, within MMJJ_RS08175 at 1502.374 kb on - strand, within MMJJ_RS08175 at 1502.421 kb on - strand at 1502.422 kb on + strand at 1502.422 kb on + strand at 1502.424 kb on - strand at 1502.425 kb on + strand at 1502.428 kb on - strand at 1502.428 kb on - strand at 1502.466 kb on - strand at 1502.466 kb on - strand at 1502.467 kb on + strand at 1502.467 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 1,499,176 - MMJJ_RS08160 0.59 -2.8 1,499,192 - MMJJ_RS08160 0.63 +0.1 1,499,229 - MMJJ_RS08160 0.72 -1.5 1,499,236 - MMJJ_RS08160 0.73 +0.8 1,499,237 + MMJJ_RS08160 0.74 -3.1 1,499,241 + MMJJ_RS08160 0.75 -3.7 1,499,241 + MMJJ_RS08160 0.75 +1.3 1,499,261 - MMJJ_RS08160 0.79 +0.7 1,499,268 - MMJJ_RS08160 0.81 +0.0 1,499,268 - MMJJ_RS08160 0.81 -0.4 1,499,268 - MMJJ_RS08160 0.81 -0.6 1,499,268 - MMJJ_RS08160 0.81 +0.2 1,499,268 - MMJJ_RS08160 0.81 +0.5 1,499,269 + MMJJ_RS08160 0.81 -4.0 1,499,269 + MMJJ_RS08160 0.81 +0.3 1,499,270 - MMJJ_RS08160 0.82 +0.4 1,499,270 - MMJJ_RS08160 0.82 +0.4 1,499,271 + MMJJ_RS08160 0.82 +1.4 1,499,271 + MMJJ_RS08160 0.82 -2.5 1,499,312 - -3.7 1,499,313 + -0.5 1,499,315 + -0.4 1,499,408 - +0.5 1,499,409 + +1.7 1,499,431 + MMJJ_RS08165 0.14 +0.9 1,499,450 + MMJJ_RS08165 0.17 -0.1 1,499,533 + MMJJ_RS08165 0.32 -0.2 1,499,534 - MMJJ_RS08165 0.32 -0.3 1,499,535 + MMJJ_RS08165 0.32 -0.4 1,499,538 + MMJJ_RS08165 0.33 -1.1 1,499,549 + MMJJ_RS08165 0.35 -0.9 1,499,599 + MMJJ_RS08165 0.44 -0.2 1,499,634 - MMJJ_RS08165 0.50 +0.2 1,499,670 + MMJJ_RS08165 0.56 +0.4 1,499,758 + MMJJ_RS08165 0.72 +0.5 1,499,758 + MMJJ_RS08165 0.72 +0.6 1,499,760 + MMJJ_RS08165 0.73 +0.9 1,499,818 - MMJJ_RS08165 0.83 -0.1 1,499,819 + MMJJ_RS08165 0.83 +0.3 1,499,822 - MMJJ_RS08165 0.84 +0.2 1,499,822 - MMJJ_RS08165 0.84 -0.1 1,499,823 + MMJJ_RS08165 0.84 +1.3 1,499,902 + -1.8 1,499,902 + -0.0 1,499,923 - +0.2 1,499,975 - +0.2 1,499,976 + -0.0 1,499,988 + -3.9 1,500,009 + -1.2 1,500,011 + -0.6 1,500,028 - -0.3 1,500,028 - +0.7 1,500,039 - +0.2 1,500,041 - +1.4 1,500,041 - +0.0 1,500,046 - +0.2 1,500,046 - -0.3 1,500,047 + -0.2 1,500,050 + -0.2 1,500,058 - -0.2 1,500,059 + +0.5 1,500,062 + +0.7 1,500,064 - -0.9 1,500,065 + -0.4 1,500,065 + +0.5 1,500,065 + +1.0 1,500,069 + +0.4 1,500,069 + +1.0 1,500,086 + +1.4 1,500,088 - +0.8 1,500,088 - +0.1 1,500,088 - -0.3 1,500,088 - +0.3 1,500,088 - +0.3 1,500,088 - -1.4 1,500,093 + -0.3 1,500,093 + +0.0 1,500,093 + -0.2 1,500,107 - -0.2 1,500,107 - +1.7 1,500,130 + +0.1 1,500,162 + +1.1 1,500,167 + +0.7 1,500,255 + -2.0 1,500,443 - MMJJ_RS08170 0.21 +0.5 1,500,473 + -0.4 1,500,768 - MMJJ_RS08170 0.46 -1.1 1,501,487 + +0.3 1,501,522 + -2.7 1,501,526 + -0.0 1,501,538 - -0.5 1,501,543 - +0.3 1,501,543 - +0.4 1,501,543 - -1.2 1,501,543 - +0.4 1,501,544 + +1.4 1,501,544 + +2.0 1,501,545 - -0.8 1,501,545 - -1.0 1,501,545 - -0.0 1,501,545 - +0.1 1,501,545 - +0.3 1,501,545 - +1.2 1,501,545 - -0.4 1,501,545 - -0.2 1,501,545 - -1.4 1,501,545 - +0.1 1,501,546 + -1.2 1,501,546 + -0.2 1,501,546 + +0.8 1,501,546 + -0.0 1,501,546 + +0.2 1,501,546 + -1.7 1,501,547 - +1.2 1,501,547 - +0.5 1,501,547 - -1.5 1,501,547 - -1.0 1,501,548 + -0.8 1,501,548 + -1.9 1,501,548 + +1.2 1,501,560 + +0.3 1,501,563 - +0.5 1,501,564 + -1.1 1,501,567 + -0.1 1,501,603 + +0.4 1,501,609 - -0.5 1,501,613 - +0.1 1,501,631 - +0.5 1,501,631 - -2.6 1,501,631 - +0.8 1,501,632 + -0.3 1,501,691 + -1.5 1,501,704 - MMJJ_RS08175 0.11 -1.0 1,501,761 - MMJJ_RS08175 0.17 -0.4 1,501,762 + MMJJ_RS08175 0.18 -0.8 1,501,838 + MMJJ_RS08175 0.26 -1.8 1,501,879 + MMJJ_RS08175 0.31 -0.5 1,501,910 + MMJJ_RS08175 0.35 -0.3 1,501,966 - MMJJ_RS08175 0.41 -1.0 1,502,014 + MMJJ_RS08175 0.47 +0.0 1,502,061 + MMJJ_RS08175 0.53 +1.2 1,502,061 + MMJJ_RS08175 0.53 -0.8 1,502,084 + MMJJ_RS08175 0.55 -1.2 1,502,084 + MMJJ_RS08175 0.55 +0.9 1,502,092 - MMJJ_RS08175 0.56 -1.1 1,502,093 + MMJJ_RS08175 0.56 -0.1 1,502,121 + MMJJ_RS08175 0.60 +1.2 1,502,131 - MMJJ_RS08175 0.61 +0.4 1,502,134 + MMJJ_RS08175 0.61 -1.0 1,502,142 - MMJJ_RS08175 0.62 +0.9 1,502,148 - MMJJ_RS08175 0.63 -3.2 1,502,185 + MMJJ_RS08175 0.67 -5.3 1,502,188 - MMJJ_RS08175 0.67 -4.3 1,502,190 - MMJJ_RS08175 0.68 -0.1 1,502,199 - MMJJ_RS08175 0.69 -0.1 1,502,205 - MMJJ_RS08175 0.69 +0.3 1,502,205 - MMJJ_RS08175 0.69 -0.6 1,502,265 + MMJJ_RS08175 0.76 -0.2 1,502,374 - MMJJ_RS08175 0.89 -0.5 1,502,421 - -0.3 1,502,422 + +0.1 1,502,422 + -1.2 1,502,424 - -0.9 1,502,425 + -0.7 1,502,428 - +1.1 1,502,428 - -0.5 1,502,466 - -1.2 1,502,466 - +1.1 1,502,467 + -2.2 1,502,467 + -1.1
Or see this region's nucleotide sequence