Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07970 and MMJJ_RS07975 are separated by 27 nucleotides MMJJ_RS07975 and MMJJ_RS07980 overlap by 4 nucleotides MMJJ_RS07980 and MMJJ_RS07985 are separated by 77 nucleotides MMJJ_RS07985 and MMJJ_RS07990 are separated by 30 nucleotides
MMJJ_RS07970: MMJJ_RS07970 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,461,221 to 1,462,363
_RS07970
MMJJ_RS07975: MMJJ_RS07975 - ATP-dependent sacrificial sulfur transferase LarE, at 1,462,391 to 1,463,167
_RS07975
MMJJ_RS07980: MMJJ_RS07980 - biotin synthase BioB, at 1,463,164 to 1,464,147
_RS07980
MMJJ_RS07985: MMJJ_RS07985 - hypothetical protein, at 1,464,225 to 1,464,425
_RS07985
MMJJ_RS07990: MMJJ_RS07990 - UPF0280 family protein, at 1,464,456 to 1,465,199
_RS07990
Position (kb)
1463
1464
1465 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1462.246 kb on + strand, within MMJJ_RS07970 at 1462.299 kb on + strand at 1462.327 kb on - strand at 1462.345 kb on - strand at 1462.364 kb on + strand at 1462.388 kb on - strand at 1462.392 kb on - strand at 1462.392 kb on - strand at 1462.393 kb on + strand at 1462.412 kb on - strand at 1462.412 kb on - strand at 1462.413 kb on + strand at 1462.431 kb on + strand at 1462.447 kb on - strand at 1462.451 kb on - strand at 1462.451 kb on - strand at 1462.454 kb on - strand at 1462.454 kb on - strand at 1462.455 kb on + strand at 1462.499 kb on + strand, within MMJJ_RS07975 at 1462.510 kb on - strand, within MMJJ_RS07975 at 1462.545 kb on - strand, within MMJJ_RS07975 at 1462.572 kb on + strand, within MMJJ_RS07975 at 1462.603 kb on - strand, within MMJJ_RS07975 at 1462.604 kb on + strand, within MMJJ_RS07975 at 1462.651 kb on - strand, within MMJJ_RS07975 at 1462.652 kb on + strand, within MMJJ_RS07975 at 1462.680 kb on - strand, within MMJJ_RS07975 at 1462.681 kb on + strand, within MMJJ_RS07975 at 1462.754 kb on + strand, within MMJJ_RS07975 at 1462.764 kb on + strand, within MMJJ_RS07975 at 1462.784 kb on - strand, within MMJJ_RS07975 at 1462.786 kb on - strand, within MMJJ_RS07975 at 1462.821 kb on - strand, within MMJJ_RS07975 at 1462.821 kb on - strand, within MMJJ_RS07975 at 1462.846 kb on - strand, within MMJJ_RS07975 at 1463.059 kb on - strand, within MMJJ_RS07975 at 1463.060 kb on + strand, within MMJJ_RS07975 at 1463.060 kb on + strand, within MMJJ_RS07975 at 1463.060 kb on + strand, within MMJJ_RS07975 at 1463.234 kb on - strand at 1463.352 kb on - strand, within MMJJ_RS07980 at 1463.353 kb on + strand, within MMJJ_RS07980 at 1463.356 kb on - strand, within MMJJ_RS07980 at 1463.392 kb on - strand, within MMJJ_RS07980 at 1463.393 kb on + strand, within MMJJ_RS07980 at 1463.394 kb on - strand, within MMJJ_RS07980 at 1463.394 kb on - strand, within MMJJ_RS07980 at 1463.394 kb on - strand, within MMJJ_RS07980 at 1463.395 kb on + strand, within MMJJ_RS07980 at 1463.407 kb on - strand, within MMJJ_RS07980 at 1463.407 kb on - strand, within MMJJ_RS07980 at 1463.413 kb on + strand, within MMJJ_RS07980 at 1463.443 kb on - strand, within MMJJ_RS07980 at 1463.446 kb on - strand, within MMJJ_RS07980 at 1463.452 kb on - strand, within MMJJ_RS07980 at 1463.453 kb on + strand, within MMJJ_RS07980 at 1463.483 kb on + strand, within MMJJ_RS07980 at 1463.491 kb on + strand, within MMJJ_RS07980 at 1463.514 kb on - strand, within MMJJ_RS07980 at 1463.514 kb on - strand at 1463.515 kb on + strand, within MMJJ_RS07980 at 1463.613 kb on - strand, within MMJJ_RS07980 at 1463.619 kb on - strand, within MMJJ_RS07980 at 1463.933 kb on + strand, within MMJJ_RS07980 at 1463.969 kb on + strand, within MMJJ_RS07980 at 1463.991 kb on - strand, within MMJJ_RS07980 at 1463.991 kb on - strand, within MMJJ_RS07980 at 1463.998 kb on + strand, within MMJJ_RS07980 at 1464.013 kb on - strand, within MMJJ_RS07980 at 1464.014 kb on + strand, within MMJJ_RS07980 at 1464.020 kb on - strand, within MMJJ_RS07980 at 1464.020 kb on - strand, within MMJJ_RS07980 at 1464.021 kb on + strand, within MMJJ_RS07980 at 1464.025 kb on - strand, within MMJJ_RS07980 at 1464.025 kb on - strand, within MMJJ_RS07980 at 1464.026 kb on + strand, within MMJJ_RS07980 at 1464.027 kb on - strand, within MMJJ_RS07980 at 1464.027 kb on - strand, within MMJJ_RS07980 at 1464.027 kb on - strand, within MMJJ_RS07980 at 1464.027 kb on - strand, within MMJJ_RS07980 at 1464.028 kb on + strand, within MMJJ_RS07980 at 1464.028 kb on + strand, within MMJJ_RS07980 at 1464.028 kb on + strand, within MMJJ_RS07980 at 1464.043 kb on - strand, within MMJJ_RS07980 at 1464.067 kb on - strand at 1464.206 kb on - strand at 1464.230 kb on - strand at 1464.230 kb on - strand at 1464.230 kb on - strand at 1464.231 kb on + strand at 1464.267 kb on + strand, within MMJJ_RS07985 at 1464.295 kb on - strand, within MMJJ_RS07985 at 1464.295 kb on - strand, within MMJJ_RS07985 at 1464.296 kb on + strand, within MMJJ_RS07985 at 1464.296 kb on + strand, within MMJJ_RS07985 at 1464.307 kb on + strand, within MMJJ_RS07985 at 1464.307 kb on + strand, within MMJJ_RS07985 at 1464.346 kb on - strand, within MMJJ_RS07985 at 1464.346 kb on - strand, within MMJJ_RS07985 at 1464.347 kb on + strand, within MMJJ_RS07985 at 1464.369 kb on - strand, within MMJJ_RS07985 at 1464.370 kb on + strand, within MMJJ_RS07985 at 1464.379 kb on + strand, within MMJJ_RS07985 at 1464.379 kb on + strand, within MMJJ_RS07985 at 1464.399 kb on - strand, within MMJJ_RS07985 at 1464.401 kb on - strand, within MMJJ_RS07985 at 1464.431 kb on + strand at 1464.439 kb on - strand at 1464.439 kb on - strand at 1464.439 kb on - strand at 1464.439 kb on - strand at 1464.440 kb on + strand at 1464.440 kb on + strand at 1464.441 kb on - strand at 1464.446 kb on - strand at 1464.447 kb on + strand at 1464.453 kb on + strand at 1464.453 kb on + strand at 1464.453 kb on + strand at 1464.550 kb on + strand, within MMJJ_RS07990 at 1465.047 kb on + strand, within MMJJ_RS07990 at 1465.139 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 1,462,246 + MMJJ_RS07970 0.90 +0.2 1,462,299 + -0.6 1,462,327 - -0.8 1,462,345 - -2.1 1,462,364 + -0.6 1,462,388 - -0.5 1,462,392 - -1.7 1,462,392 - +0.1 1,462,393 + +0.5 1,462,412 - -0.1 1,462,412 - -1.2 1,462,413 + +1.2 1,462,431 + +2.2 1,462,447 - +0.4 1,462,451 - -0.6 1,462,451 - -0.5 1,462,454 - -0.1 1,462,454 - -0.9 1,462,455 + +1.3 1,462,499 + MMJJ_RS07975 0.14 -1.4 1,462,510 - MMJJ_RS07975 0.15 -5.5 1,462,545 - MMJJ_RS07975 0.20 +0.1 1,462,572 + MMJJ_RS07975 0.23 +1.6 1,462,603 - MMJJ_RS07975 0.27 -0.1 1,462,604 + MMJJ_RS07975 0.27 -1.5 1,462,651 - MMJJ_RS07975 0.33 -2.1 1,462,652 + MMJJ_RS07975 0.34 -0.6 1,462,680 - MMJJ_RS07975 0.37 +1.6 1,462,681 + MMJJ_RS07975 0.37 -1.7 1,462,754 + MMJJ_RS07975 0.47 +0.1 1,462,764 + MMJJ_RS07975 0.48 -0.2 1,462,784 - MMJJ_RS07975 0.51 -0.5 1,462,786 - MMJJ_RS07975 0.51 +0.2 1,462,821 - MMJJ_RS07975 0.55 +0.8 1,462,821 - MMJJ_RS07975 0.55 -0.1 1,462,846 - MMJJ_RS07975 0.59 -1.4 1,463,059 - MMJJ_RS07975 0.86 -0.3 1,463,060 + MMJJ_RS07975 0.86 +0.5 1,463,060 + MMJJ_RS07975 0.86 -0.0 1,463,060 + MMJJ_RS07975 0.86 +1.3 1,463,234 - -0.5 1,463,352 - MMJJ_RS07980 0.19 +1.5 1,463,353 + MMJJ_RS07980 0.19 -2.8 1,463,356 - MMJJ_RS07980 0.20 -1.8 1,463,392 - MMJJ_RS07980 0.23 -0.6 1,463,393 + MMJJ_RS07980 0.23 -0.2 1,463,394 - MMJJ_RS07980 0.23 +3.2 1,463,394 - MMJJ_RS07980 0.23 -0.8 1,463,394 - MMJJ_RS07980 0.23 -0.6 1,463,395 + MMJJ_RS07980 0.23 -2.2 1,463,407 - MMJJ_RS07980 0.25 +0.8 1,463,407 - MMJJ_RS07980 0.25 +0.2 1,463,413 + MMJJ_RS07980 0.25 -0.0 1,463,443 - MMJJ_RS07980 0.28 +0.4 1,463,446 - MMJJ_RS07980 0.29 +3.5 1,463,452 - MMJJ_RS07980 0.29 -2.1 1,463,453 + MMJJ_RS07980 0.29 -0.2 1,463,483 + MMJJ_RS07980 0.32 -0.2 1,463,491 + MMJJ_RS07980 0.33 -4.1 1,463,514 - MMJJ_RS07980 0.36 -3.8 1,463,514 - -0.6 1,463,515 + MMJJ_RS07980 0.36 -0.3 1,463,613 - MMJJ_RS07980 0.46 +0.9 1,463,619 - MMJJ_RS07980 0.46 -0.2 1,463,933 + MMJJ_RS07980 0.78 -2.1 1,463,969 + MMJJ_RS07980 0.82 +1.9 1,463,991 - MMJJ_RS07980 0.84 +1.6 1,463,991 - MMJJ_RS07980 0.84 -4.1 1,463,998 + MMJJ_RS07980 0.85 -1.1 1,464,013 - MMJJ_RS07980 0.86 -1.0 1,464,014 + MMJJ_RS07980 0.86 +1.9 1,464,020 - MMJJ_RS07980 0.87 +0.9 1,464,020 - MMJJ_RS07980 0.87 -1.8 1,464,021 + MMJJ_RS07980 0.87 -3.1 1,464,025 - MMJJ_RS07980 0.88 +2.0 1,464,025 - MMJJ_RS07980 0.88 -3.7 1,464,026 + MMJJ_RS07980 0.88 +0.3 1,464,027 - MMJJ_RS07980 0.88 -4.1 1,464,027 - MMJJ_RS07980 0.88 +1.4 1,464,027 - MMJJ_RS07980 0.88 -3.9 1,464,027 - MMJJ_RS07980 0.88 +0.7 1,464,028 + MMJJ_RS07980 0.88 -2.3 1,464,028 + MMJJ_RS07980 0.88 +0.1 1,464,028 + MMJJ_RS07980 0.88 +0.0 1,464,043 - MMJJ_RS07980 0.89 -0.5 1,464,067 - -1.1 1,464,206 - +1.0 1,464,230 - -0.5 1,464,230 - +0.3 1,464,230 - -0.3 1,464,231 + -2.2 1,464,267 + MMJJ_RS07985 0.21 +1.7 1,464,295 - MMJJ_RS07985 0.35 +0.5 1,464,295 - MMJJ_RS07985 0.35 -0.2 1,464,296 + MMJJ_RS07985 0.35 +0.6 1,464,296 + MMJJ_RS07985 0.35 +0.2 1,464,307 + MMJJ_RS07985 0.41 +0.8 1,464,307 + MMJJ_RS07985 0.41 +0.5 1,464,346 - MMJJ_RS07985 0.60 +1.1 1,464,346 - MMJJ_RS07985 0.60 -1.4 1,464,347 + MMJJ_RS07985 0.61 -1.3 1,464,369 - MMJJ_RS07985 0.72 -1.1 1,464,370 + MMJJ_RS07985 0.72 -1.1 1,464,379 + MMJJ_RS07985 0.77 +0.7 1,464,379 + MMJJ_RS07985 0.77 -1.1 1,464,399 - MMJJ_RS07985 0.87 -0.3 1,464,401 - MMJJ_RS07985 0.88 +0.2 1,464,431 + +0.1 1,464,439 - -0.2 1,464,439 - -1.1 1,464,439 - -0.3 1,464,439 - -1.0 1,464,440 + -0.6 1,464,440 + -0.6 1,464,441 - -0.1 1,464,446 - -0.0 1,464,447 + +0.2 1,464,453 + +0.4 1,464,453 + -2.5 1,464,453 + +1.4 1,464,550 + MMJJ_RS07990 0.13 -1.3 1,465,047 + MMJJ_RS07990 0.79 -1.0 1,465,139 - -0.4
Or see this region's nucleotide sequence