Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS07980

Experiment: McCas-formate glass attached

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS07970 and MMJJ_RS07975 are separated by 27 nucleotidesMMJJ_RS07975 and MMJJ_RS07980 overlap by 4 nucleotidesMMJJ_RS07980 and MMJJ_RS07985 are separated by 77 nucleotidesMMJJ_RS07985 and MMJJ_RS07990 are separated by 30 nucleotides MMJJ_RS07970: MMJJ_RS07970 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,461,221 to 1,462,363 _RS07970 MMJJ_RS07975: MMJJ_RS07975 - ATP-dependent sacrificial sulfur transferase LarE, at 1,462,391 to 1,463,167 _RS07975 MMJJ_RS07980: MMJJ_RS07980 - biotin synthase BioB, at 1,463,164 to 1,464,147 _RS07980 MMJJ_RS07985: MMJJ_RS07985 - hypothetical protein, at 1,464,225 to 1,464,425 _RS07985 MMJJ_RS07990: MMJJ_RS07990 - UPF0280 family protein, at 1,464,456 to 1,465,199 _RS07990 Position (kb) 1463 1464 1465Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 1462.246 kb on + strand, within MMJJ_RS07970at 1462.299 kb on + strandat 1462.327 kb on - strandat 1462.345 kb on - strandat 1462.364 kb on + strandat 1462.388 kb on - strandat 1462.392 kb on - strandat 1462.392 kb on - strandat 1462.393 kb on + strandat 1462.412 kb on - strandat 1462.412 kb on - strandat 1462.413 kb on + strandat 1462.431 kb on + strandat 1462.447 kb on - strandat 1462.451 kb on - strandat 1462.451 kb on - strandat 1462.454 kb on - strandat 1462.454 kb on - strandat 1462.455 kb on + strandat 1462.499 kb on + strand, within MMJJ_RS07975at 1462.510 kb on - strand, within MMJJ_RS07975at 1462.545 kb on - strand, within MMJJ_RS07975at 1462.572 kb on + strand, within MMJJ_RS07975at 1462.603 kb on - strand, within MMJJ_RS07975at 1462.604 kb on + strand, within MMJJ_RS07975at 1462.651 kb on - strand, within MMJJ_RS07975at 1462.652 kb on + strand, within MMJJ_RS07975at 1462.680 kb on - strand, within MMJJ_RS07975at 1462.681 kb on + strand, within MMJJ_RS07975at 1462.754 kb on + strand, within MMJJ_RS07975at 1462.764 kb on + strand, within MMJJ_RS07975at 1462.784 kb on - strand, within MMJJ_RS07975at 1462.786 kb on - strand, within MMJJ_RS07975at 1462.821 kb on - strand, within MMJJ_RS07975at 1462.821 kb on - strand, within MMJJ_RS07975at 1462.846 kb on - strand, within MMJJ_RS07975at 1463.059 kb on - strand, within MMJJ_RS07975at 1463.060 kb on + strand, within MMJJ_RS07975at 1463.060 kb on + strand, within MMJJ_RS07975at 1463.060 kb on + strand, within MMJJ_RS07975at 1463.234 kb on - strandat 1463.352 kb on - strand, within MMJJ_RS07980at 1463.353 kb on + strand, within MMJJ_RS07980at 1463.356 kb on - strand, within MMJJ_RS07980at 1463.392 kb on - strand, within MMJJ_RS07980at 1463.393 kb on + strand, within MMJJ_RS07980at 1463.394 kb on - strand, within MMJJ_RS07980at 1463.394 kb on - strand, within MMJJ_RS07980at 1463.394 kb on - strand, within MMJJ_RS07980at 1463.395 kb on + strand, within MMJJ_RS07980at 1463.407 kb on - strand, within MMJJ_RS07980at 1463.407 kb on - strand, within MMJJ_RS07980at 1463.413 kb on + strand, within MMJJ_RS07980at 1463.443 kb on - strand, within MMJJ_RS07980at 1463.446 kb on - strand, within MMJJ_RS07980at 1463.452 kb on - strand, within MMJJ_RS07980at 1463.453 kb on + strand, within MMJJ_RS07980at 1463.483 kb on + strand, within MMJJ_RS07980at 1463.491 kb on + strand, within MMJJ_RS07980at 1463.514 kb on - strand, within MMJJ_RS07980at 1463.514 kb on - strandat 1463.515 kb on + strand, within MMJJ_RS07980at 1463.613 kb on - strand, within MMJJ_RS07980at 1463.619 kb on - strand, within MMJJ_RS07980at 1463.933 kb on + strand, within MMJJ_RS07980at 1463.969 kb on + strand, within MMJJ_RS07980at 1463.991 kb on - strand, within MMJJ_RS07980at 1463.991 kb on - strand, within MMJJ_RS07980at 1463.998 kb on + strand, within MMJJ_RS07980at 1464.013 kb on - strand, within MMJJ_RS07980at 1464.014 kb on + strand, within MMJJ_RS07980at 1464.020 kb on - strand, within MMJJ_RS07980at 1464.020 kb on - strand, within MMJJ_RS07980at 1464.021 kb on + strand, within MMJJ_RS07980at 1464.025 kb on - strand, within MMJJ_RS07980at 1464.025 kb on - strand, within MMJJ_RS07980at 1464.026 kb on + strand, within MMJJ_RS07980at 1464.027 kb on - strand, within MMJJ_RS07980at 1464.027 kb on - strand, within MMJJ_RS07980at 1464.027 kb on - strand, within MMJJ_RS07980at 1464.027 kb on - strand, within MMJJ_RS07980at 1464.028 kb on + strand, within MMJJ_RS07980at 1464.028 kb on + strand, within MMJJ_RS07980at 1464.028 kb on + strand, within MMJJ_RS07980at 1464.043 kb on - strand, within MMJJ_RS07980at 1464.067 kb on - strandat 1464.206 kb on - strandat 1464.230 kb on - strandat 1464.230 kb on - strandat 1464.230 kb on - strandat 1464.231 kb on + strandat 1464.267 kb on + strand, within MMJJ_RS07985at 1464.295 kb on - strand, within MMJJ_RS07985at 1464.295 kb on - strand, within MMJJ_RS07985at 1464.296 kb on + strand, within MMJJ_RS07985at 1464.296 kb on + strand, within MMJJ_RS07985at 1464.307 kb on + strand, within MMJJ_RS07985at 1464.307 kb on + strand, within MMJJ_RS07985at 1464.346 kb on - strand, within MMJJ_RS07985at 1464.346 kb on - strand, within MMJJ_RS07985at 1464.347 kb on + strand, within MMJJ_RS07985at 1464.369 kb on - strand, within MMJJ_RS07985at 1464.370 kb on + strand, within MMJJ_RS07985at 1464.379 kb on + strand, within MMJJ_RS07985at 1464.379 kb on + strand, within MMJJ_RS07985at 1464.399 kb on - strand, within MMJJ_RS07985at 1464.401 kb on - strand, within MMJJ_RS07985at 1464.431 kb on + strandat 1464.439 kb on - strandat 1464.439 kb on - strandat 1464.439 kb on - strandat 1464.439 kb on - strandat 1464.440 kb on + strandat 1464.440 kb on + strandat 1464.441 kb on - strandat 1464.446 kb on - strandat 1464.447 kb on + strandat 1464.453 kb on + strandat 1464.453 kb on + strandat 1464.453 kb on + strandat 1464.550 kb on + strand, within MMJJ_RS07990at 1465.047 kb on + strand, within MMJJ_RS07990at 1465.139 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas-formate glass attached
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1,462,246 + MMJJ_RS07970 0.90 +0.2
1,462,299 + -0.6
1,462,327 - -0.8
1,462,345 - -2.1
1,462,364 + -0.6
1,462,388 - -0.5
1,462,392 - -1.7
1,462,392 - +0.1
1,462,393 + +0.5
1,462,412 - -0.1
1,462,412 - -1.2
1,462,413 + +1.2
1,462,431 + +2.2
1,462,447 - +0.4
1,462,451 - -0.6
1,462,451 - -0.5
1,462,454 - -0.1
1,462,454 - -0.9
1,462,455 + +1.3
1,462,499 + MMJJ_RS07975 0.14 -1.4
1,462,510 - MMJJ_RS07975 0.15 -5.5
1,462,545 - MMJJ_RS07975 0.20 +0.1
1,462,572 + MMJJ_RS07975 0.23 +1.6
1,462,603 - MMJJ_RS07975 0.27 -0.1
1,462,604 + MMJJ_RS07975 0.27 -1.5
1,462,651 - MMJJ_RS07975 0.33 -2.1
1,462,652 + MMJJ_RS07975 0.34 -0.6
1,462,680 - MMJJ_RS07975 0.37 +1.6
1,462,681 + MMJJ_RS07975 0.37 -1.7
1,462,754 + MMJJ_RS07975 0.47 +0.1
1,462,764 + MMJJ_RS07975 0.48 -0.2
1,462,784 - MMJJ_RS07975 0.51 -0.5
1,462,786 - MMJJ_RS07975 0.51 +0.2
1,462,821 - MMJJ_RS07975 0.55 +0.8
1,462,821 - MMJJ_RS07975 0.55 -0.1
1,462,846 - MMJJ_RS07975 0.59 -1.4
1,463,059 - MMJJ_RS07975 0.86 -0.3
1,463,060 + MMJJ_RS07975 0.86 +0.5
1,463,060 + MMJJ_RS07975 0.86 -0.0
1,463,060 + MMJJ_RS07975 0.86 +1.3
1,463,234 - -0.5
1,463,352 - MMJJ_RS07980 0.19 +1.5
1,463,353 + MMJJ_RS07980 0.19 -2.8
1,463,356 - MMJJ_RS07980 0.20 -1.8
1,463,392 - MMJJ_RS07980 0.23 -0.6
1,463,393 + MMJJ_RS07980 0.23 -0.2
1,463,394 - MMJJ_RS07980 0.23 +3.2
1,463,394 - MMJJ_RS07980 0.23 -0.8
1,463,394 - MMJJ_RS07980 0.23 -0.6
1,463,395 + MMJJ_RS07980 0.23 -2.2
1,463,407 - MMJJ_RS07980 0.25 +0.8
1,463,407 - MMJJ_RS07980 0.25 +0.2
1,463,413 + MMJJ_RS07980 0.25 -0.0
1,463,443 - MMJJ_RS07980 0.28 +0.4
1,463,446 - MMJJ_RS07980 0.29 +3.5
1,463,452 - MMJJ_RS07980 0.29 -2.1
1,463,453 + MMJJ_RS07980 0.29 -0.2
1,463,483 + MMJJ_RS07980 0.32 -0.2
1,463,491 + MMJJ_RS07980 0.33 -4.1
1,463,514 - MMJJ_RS07980 0.36 -3.8
1,463,514 - -0.6
1,463,515 + MMJJ_RS07980 0.36 -0.3
1,463,613 - MMJJ_RS07980 0.46 +0.9
1,463,619 - MMJJ_RS07980 0.46 -0.2
1,463,933 + MMJJ_RS07980 0.78 -2.1
1,463,969 + MMJJ_RS07980 0.82 +1.9
1,463,991 - MMJJ_RS07980 0.84 +1.6
1,463,991 - MMJJ_RS07980 0.84 -4.1
1,463,998 + MMJJ_RS07980 0.85 -1.1
1,464,013 - MMJJ_RS07980 0.86 -1.0
1,464,014 + MMJJ_RS07980 0.86 +1.9
1,464,020 - MMJJ_RS07980 0.87 +0.9
1,464,020 - MMJJ_RS07980 0.87 -1.8
1,464,021 + MMJJ_RS07980 0.87 -3.1
1,464,025 - MMJJ_RS07980 0.88 +2.0
1,464,025 - MMJJ_RS07980 0.88 -3.7
1,464,026 + MMJJ_RS07980 0.88 +0.3
1,464,027 - MMJJ_RS07980 0.88 -4.1
1,464,027 - MMJJ_RS07980 0.88 +1.4
1,464,027 - MMJJ_RS07980 0.88 -3.9
1,464,027 - MMJJ_RS07980 0.88 +0.7
1,464,028 + MMJJ_RS07980 0.88 -2.3
1,464,028 + MMJJ_RS07980 0.88 +0.1
1,464,028 + MMJJ_RS07980 0.88 +0.0
1,464,043 - MMJJ_RS07980 0.89 -0.5
1,464,067 - -1.1
1,464,206 - +1.0
1,464,230 - -0.5
1,464,230 - +0.3
1,464,230 - -0.3
1,464,231 + -2.2
1,464,267 + MMJJ_RS07985 0.21 +1.7
1,464,295 - MMJJ_RS07985 0.35 +0.5
1,464,295 - MMJJ_RS07985 0.35 -0.2
1,464,296 + MMJJ_RS07985 0.35 +0.6
1,464,296 + MMJJ_RS07985 0.35 +0.2
1,464,307 + MMJJ_RS07985 0.41 +0.8
1,464,307 + MMJJ_RS07985 0.41 +0.5
1,464,346 - MMJJ_RS07985 0.60 +1.1
1,464,346 - MMJJ_RS07985 0.60 -1.4
1,464,347 + MMJJ_RS07985 0.61 -1.3
1,464,369 - MMJJ_RS07985 0.72 -1.1
1,464,370 + MMJJ_RS07985 0.72 -1.1
1,464,379 + MMJJ_RS07985 0.77 +0.7
1,464,379 + MMJJ_RS07985 0.77 -1.1
1,464,399 - MMJJ_RS07985 0.87 -0.3
1,464,401 - MMJJ_RS07985 0.88 +0.2
1,464,431 + +0.1
1,464,439 - -0.2
1,464,439 - -1.1
1,464,439 - -0.3
1,464,439 - -1.0
1,464,440 + -0.6
1,464,440 + -0.6
1,464,441 - -0.1
1,464,446 - -0.0
1,464,447 + +0.2
1,464,453 + +0.4
1,464,453 + -2.5
1,464,453 + +1.4
1,464,550 + MMJJ_RS07990 0.13 -1.3
1,465,047 + MMJJ_RS07990 0.79 -1.0
1,465,139 - -0.4

Or see this region's nucleotide sequence