Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07220 and MMJJ_RS07225 are separated by 33 nucleotides MMJJ_RS07225 and MMJJ_RS07230 are separated by 14 nucleotides MMJJ_RS07230 and MMJJ_RS07235 are separated by 101 nucleotides
MMJJ_RS07220: MMJJ_RS07220 - coenzyme F420 hydrogenase subunit beta, at 1,322,153 to 1,323,001
_RS07220
MMJJ_RS07225: MMJJ_RS07225 - coenzyme F420 hydrogenase subunit gamma, at 1,323,035 to 1,323,721
_RS07225
MMJJ_RS07230: MMJJ_RS07230 - coenzyme F420-reducing hydrogenase, FrhD protein, at 1,323,736 to 1,324,260
_RS07230
MMJJ_RS07235: MMJJ_RS07235 - coenzyme F420 hydrogenase subunit alpha, at 1,324,362 to 1,325,606
_RS07235
Position (kb)
1323
1324
1325 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 1322.736 kb on + strand, within MMJJ_RS07220 at 1322.747 kb on - strand, within MMJJ_RS07220 at 1322.762 kb on + strand, within MMJJ_RS07220 at 1322.768 kb on - strand, within MMJJ_RS07220 at 1322.768 kb on - strand, within MMJJ_RS07220 at 1322.768 kb on - strand, within MMJJ_RS07220 at 1322.769 kb on + strand, within MMJJ_RS07220 at 1322.769 kb on + strand, within MMJJ_RS07220 at 1322.773 kb on - strand, within MMJJ_RS07220 at 1322.774 kb on + strand, within MMJJ_RS07220 at 1322.775 kb on - strand, within MMJJ_RS07220 at 1322.776 kb on + strand, within MMJJ_RS07220 at 1322.779 kb on - strand, within MMJJ_RS07220 at 1322.815 kb on - strand, within MMJJ_RS07220 at 1322.864 kb on - strand, within MMJJ_RS07220 at 1322.865 kb on + strand, within MMJJ_RS07220 at 1322.902 kb on + strand, within MMJJ_RS07220 at 1322.979 kb on + strand at 1322.979 kb on + strand at 1322.980 kb on - strand at 1322.983 kb on + strand at 1323.003 kb on - strand at 1323.004 kb on + strand at 1323.004 kb on + strand at 1323.004 kb on + strand at 1323.004 kb on + strand at 1323.017 kb on - strand at 1323.027 kb on + strand at 1323.037 kb on + strand at 1323.053 kb on - strand at 1323.053 kb on - strand at 1323.145 kb on - strand, within MMJJ_RS07225 at 1323.145 kb on - strand, within MMJJ_RS07225 at 1323.146 kb on + strand, within MMJJ_RS07225 at 1323.149 kb on - strand, within MMJJ_RS07225 at 1323.149 kb on - strand, within MMJJ_RS07225 at 1323.150 kb on + strand, within MMJJ_RS07225 at 1323.150 kb on + strand, within MMJJ_RS07225 at 1323.150 kb on + strand, within MMJJ_RS07225 at 1323.160 kb on - strand, within MMJJ_RS07225 at 1323.194 kb on + strand, within MMJJ_RS07225 at 1323.213 kb on + strand, within MMJJ_RS07225 at 1323.218 kb on - strand, within MMJJ_RS07225 at 1323.278 kb on - strand, within MMJJ_RS07225 at 1323.279 kb on + strand, within MMJJ_RS07225 at 1323.279 kb on + strand, within MMJJ_RS07225 at 1323.283 kb on + strand, within MMJJ_RS07225 at 1323.323 kb on - strand, within MMJJ_RS07225 at 1323.442 kb on - strand, within MMJJ_RS07225 at 1323.442 kb on - strand, within MMJJ_RS07225 at 1323.479 kb on - strand, within MMJJ_RS07225 at 1323.480 kb on + strand, within MMJJ_RS07225 at 1323.548 kb on + strand, within MMJJ_RS07225 at 1323.548 kb on + strand, within MMJJ_RS07225 at 1323.563 kb on - strand, within MMJJ_RS07225 at 1323.563 kb on - strand, within MMJJ_RS07225 at 1323.563 kb on - strand, within MMJJ_RS07225 at 1323.564 kb on + strand, within MMJJ_RS07225 at 1323.564 kb on + strand, within MMJJ_RS07225 at 1323.569 kb on - strand, within MMJJ_RS07225 at 1323.570 kb on + strand, within MMJJ_RS07225 at 1323.617 kb on - strand, within MMJJ_RS07225 at 1323.623 kb on - strand, within MMJJ_RS07225 at 1323.624 kb on + strand, within MMJJ_RS07225 at 1323.641 kb on - strand, within MMJJ_RS07225 at 1323.642 kb on + strand, within MMJJ_RS07225 at 1323.674 kb on - strand at 1323.695 kb on - strand at 1323.695 kb on - strand at 1323.695 kb on - strand at 1323.696 kb on + strand at 1323.725 kb on + strand at 1323.737 kb on - strand at 1323.738 kb on + strand at 1323.824 kb on + strand, within MMJJ_RS07230 at 1323.824 kb on + strand, within MMJJ_RS07230 at 1323.826 kb on - strand, within MMJJ_RS07230 at 1323.826 kb on - strand, within MMJJ_RS07230 at 1323.845 kb on - strand, within MMJJ_RS07230 at 1323.845 kb on - strand, within MMJJ_RS07230 at 1323.846 kb on + strand, within MMJJ_RS07230 at 1323.871 kb on + strand, within MMJJ_RS07230 at 1323.898 kb on + strand, within MMJJ_RS07230 at 1323.901 kb on - strand, within MMJJ_RS07230 at 1323.902 kb on + strand, within MMJJ_RS07230 at 1323.909 kb on + strand, within MMJJ_RS07230 at 1323.909 kb on + strand, within MMJJ_RS07230 at 1323.909 kb on + strand, within MMJJ_RS07230 at 1323.915 kb on - strand, within MMJJ_RS07230 at 1323.981 kb on - strand, within MMJJ_RS07230 at 1323.981 kb on - strand, within MMJJ_RS07230 at 1323.981 kb on - strand, within MMJJ_RS07230 at 1323.982 kb on + strand, within MMJJ_RS07230 at 1323.982 kb on + strand, within MMJJ_RS07230 at 1323.982 kb on + strand, within MMJJ_RS07230 at 1323.986 kb on + strand, within MMJJ_RS07230 at 1323.990 kb on - strand, within MMJJ_RS07230 at 1323.991 kb on + strand, within MMJJ_RS07230 at 1324.033 kb on - strand, within MMJJ_RS07230 at 1324.141 kb on - strand, within MMJJ_RS07230 at 1324.173 kb on - strand, within MMJJ_RS07230 at 1324.174 kb on + strand, within MMJJ_RS07230 at 1324.197 kb on - strand, within MMJJ_RS07230 at 1324.219 kb on + strand at 1324.228 kb on - strand at 1324.236 kb on + strand at 1324.253 kb on - strand at 1324.287 kb on + strand at 1324.287 kb on + strand at 1324.298 kb on + strand at 1324.302 kb on - strand at 1324.327 kb on + strand at 1324.374 kb on - strand at 1324.422 kb on + strand at 1324.437 kb on - strand at 1324.438 kb on + strand at 1324.438 kb on + strand at 1324.444 kb on - strand at 1324.444 kb on - strand at 1324.454 kb on - strand at 1324.455 kb on + strand at 1324.469 kb on + strand at 1324.469 kb on + strand at 1324.473 kb on + strand at 1324.473 kb on + strand at 1324.473 kb on + strand at 1324.473 kb on + strand at 1324.540 kb on - strand, within MMJJ_RS07235 at 1324.540 kb on - strand, within MMJJ_RS07235 at 1324.541 kb on + strand, within MMJJ_RS07235 at 1324.639 kb on - strand, within MMJJ_RS07235 at 1324.639 kb on - strand, within MMJJ_RS07235 at 1324.639 kb on - strand, within MMJJ_RS07235 at 1324.646 kb on - strand, within MMJJ_RS07235 at 1324.647 kb on + strand, within MMJJ_RS07235 at 1324.647 kb on + strand, within MMJJ_RS07235 at 1324.647 kb on + strand, within MMJJ_RS07235 at 1324.650 kb on - strand, within MMJJ_RS07235 at 1324.650 kb on - strand, within MMJJ_RS07235 at 1324.651 kb on + strand, within MMJJ_RS07235 at 1324.659 kb on - strand, within MMJJ_RS07235 at 1324.659 kb on - strand at 1324.689 kb on + strand, within MMJJ_RS07235 at 1324.785 kb on + strand, within MMJJ_RS07235 at 1324.800 kb on + strand, within MMJJ_RS07235 at 1324.828 kb on - strand, within MMJJ_RS07235 at 1324.879 kb on - strand, within MMJJ_RS07235 at 1324.880 kb on + strand, within MMJJ_RS07235 at 1324.880 kb on + strand, within MMJJ_RS07235 at 1324.882 kb on - strand, within MMJJ_RS07235 at 1324.882 kb on - strand, within MMJJ_RS07235 at 1324.882 kb on - strand, within MMJJ_RS07235 at 1324.896 kb on - strand, within MMJJ_RS07235 at 1324.896 kb on - strand, within MMJJ_RS07235 at 1324.905 kb on - strand, within MMJJ_RS07235 at 1324.906 kb on + strand, within MMJJ_RS07235 at 1324.923 kb on + strand, within MMJJ_RS07235 at 1324.952 kb on - strand, within MMJJ_RS07235 at 1324.953 kb on + strand, within MMJJ_RS07235 at 1324.961 kb on + strand, within MMJJ_RS07235 at 1324.976 kb on - strand, within MMJJ_RS07235 at 1324.976 kb on - strand, within MMJJ_RS07235 at 1324.980 kb on - strand, within MMJJ_RS07235 at 1324.981 kb on + strand, within MMJJ_RS07235 at 1325.004 kb on - strand, within MMJJ_RS07235 at 1325.004 kb on - strand, within MMJJ_RS07235 at 1325.004 kb on - strand, within MMJJ_RS07235 at 1325.005 kb on + strand, within MMJJ_RS07235 at 1325.005 kb on + strand, within MMJJ_RS07235 at 1325.005 kb on + strand, within MMJJ_RS07235 at 1325.031 kb on - strand, within MMJJ_RS07235 at 1325.038 kb on + strand, within MMJJ_RS07235 at 1325.048 kb on - strand, within MMJJ_RS07235 at 1325.049 kb on + strand, within MMJJ_RS07235 at 1325.049 kb on + strand, within MMJJ_RS07235 at 1325.049 kb on + strand, within MMJJ_RS07235 at 1325.063 kb on + strand, within MMJJ_RS07235 at 1325.070 kb on + strand, within MMJJ_RS07235 at 1325.077 kb on - strand, within MMJJ_RS07235 at 1325.077 kb on - strand, within MMJJ_RS07235 at 1325.077 kb on - strand, within MMJJ_RS07235 at 1325.078 kb on + strand, within MMJJ_RS07235 at 1325.078 kb on + strand, within MMJJ_RS07235 at 1325.078 kb on + strand at 1325.078 kb on + strand, within MMJJ_RS07235 at 1325.080 kb on - strand, within MMJJ_RS07235 at 1325.166 kb on + strand, within MMJJ_RS07235 at 1325.172 kb on - strand, within MMJJ_RS07235 at 1325.172 kb on - strand, within MMJJ_RS07235 at 1325.172 kb on - strand, within MMJJ_RS07235 at 1325.173 kb on + strand, within MMJJ_RS07235 at 1325.229 kb on - strand, within MMJJ_RS07235
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 1,322,736 + MMJJ_RS07220 0.69 -2.1 1,322,747 - MMJJ_RS07220 0.70 -0.2 1,322,762 + MMJJ_RS07220 0.72 +0.1 1,322,768 - MMJJ_RS07220 0.72 +0.5 1,322,768 - MMJJ_RS07220 0.72 +0.7 1,322,768 - MMJJ_RS07220 0.72 -0.9 1,322,769 + MMJJ_RS07220 0.73 +1.1 1,322,769 + MMJJ_RS07220 0.73 -0.2 1,322,773 - MMJJ_RS07220 0.73 -1.8 1,322,774 + MMJJ_RS07220 0.73 +1.2 1,322,775 - MMJJ_RS07220 0.73 +0.5 1,322,776 + MMJJ_RS07220 0.73 +0.4 1,322,779 - MMJJ_RS07220 0.74 -1.3 1,322,815 - MMJJ_RS07220 0.78 -0.3 1,322,864 - MMJJ_RS07220 0.84 +0.3 1,322,865 + MMJJ_RS07220 0.84 +2.5 1,322,902 + MMJJ_RS07220 0.88 -0.9 1,322,979 + -0.7 1,322,979 + +0.5 1,322,980 - +2.8 1,322,983 + -0.1 1,323,003 - -5.2 1,323,004 + -1.1 1,323,004 + -0.3 1,323,004 + +0.6 1,323,004 + -1.6 1,323,017 - +1.3 1,323,027 + +0.2 1,323,037 + -0.4 1,323,053 - +0.4 1,323,053 - -0.7 1,323,145 - MMJJ_RS07225 0.16 +0.1 1,323,145 - MMJJ_RS07225 0.16 +0.5 1,323,146 + MMJJ_RS07225 0.16 -0.2 1,323,149 - MMJJ_RS07225 0.17 +0.0 1,323,149 - MMJJ_RS07225 0.17 -0.3 1,323,150 + MMJJ_RS07225 0.17 -2.3 1,323,150 + MMJJ_RS07225 0.17 +0.1 1,323,150 + MMJJ_RS07225 0.17 -4.0 1,323,160 - MMJJ_RS07225 0.18 +0.6 1,323,194 + MMJJ_RS07225 0.23 +0.6 1,323,213 + MMJJ_RS07225 0.26 -0.1 1,323,218 - MMJJ_RS07225 0.27 +0.2 1,323,278 - MMJJ_RS07225 0.35 -1.2 1,323,279 + MMJJ_RS07225 0.36 +1.2 1,323,279 + MMJJ_RS07225 0.36 +0.8 1,323,283 + MMJJ_RS07225 0.36 +0.5 1,323,323 - MMJJ_RS07225 0.42 +1.3 1,323,442 - MMJJ_RS07225 0.59 -0.0 1,323,442 - MMJJ_RS07225 0.59 -0.6 1,323,479 - MMJJ_RS07225 0.65 +0.9 1,323,480 + MMJJ_RS07225 0.65 -1.1 1,323,548 + MMJJ_RS07225 0.75 -1.9 1,323,548 + MMJJ_RS07225 0.75 -1.1 1,323,563 - MMJJ_RS07225 0.77 -0.9 1,323,563 - MMJJ_RS07225 0.77 -0.3 1,323,563 - MMJJ_RS07225 0.77 +1.0 1,323,564 + MMJJ_RS07225 0.77 +0.4 1,323,564 + MMJJ_RS07225 0.77 +0.1 1,323,569 - MMJJ_RS07225 0.78 -0.2 1,323,570 + MMJJ_RS07225 0.78 -0.6 1,323,617 - MMJJ_RS07225 0.85 +1.2 1,323,623 - MMJJ_RS07225 0.86 +0.4 1,323,624 + MMJJ_RS07225 0.86 +0.7 1,323,641 - MMJJ_RS07225 0.88 +0.8 1,323,642 + MMJJ_RS07225 0.88 -0.6 1,323,674 - +0.7 1,323,695 - -0.1 1,323,695 - +0.5 1,323,695 - +1.3 1,323,696 + -1.5 1,323,725 + -0.2 1,323,737 - +0.2 1,323,738 + +0.1 1,323,824 + MMJJ_RS07230 0.17 +0.7 1,323,824 + MMJJ_RS07230 0.17 +1.9 1,323,826 - MMJJ_RS07230 0.17 -1.6 1,323,826 - MMJJ_RS07230 0.17 -0.6 1,323,845 - MMJJ_RS07230 0.21 -1.6 1,323,845 - MMJJ_RS07230 0.21 +0.5 1,323,846 + MMJJ_RS07230 0.21 +0.0 1,323,871 + MMJJ_RS07230 0.26 -3.7 1,323,898 + MMJJ_RS07230 0.31 +0.8 1,323,901 - MMJJ_RS07230 0.31 +0.2 1,323,902 + MMJJ_RS07230 0.32 +0.3 1,323,909 + MMJJ_RS07230 0.33 -1.2 1,323,909 + MMJJ_RS07230 0.33 +0.4 1,323,909 + MMJJ_RS07230 0.33 -0.0 1,323,915 - MMJJ_RS07230 0.34 -0.8 1,323,981 - MMJJ_RS07230 0.47 +0.4 1,323,981 - MMJJ_RS07230 0.47 +0.7 1,323,981 - MMJJ_RS07230 0.47 +0.2 1,323,982 + MMJJ_RS07230 0.47 +1.1 1,323,982 + MMJJ_RS07230 0.47 +0.1 1,323,982 + MMJJ_RS07230 0.47 +0.2 1,323,986 + MMJJ_RS07230 0.48 +1.2 1,323,990 - MMJJ_RS07230 0.48 +0.2 1,323,991 + MMJJ_RS07230 0.49 +1.2 1,324,033 - MMJJ_RS07230 0.57 -4.0 1,324,141 - MMJJ_RS07230 0.77 +0.2 1,324,173 - MMJJ_RS07230 0.83 +0.8 1,324,174 + MMJJ_RS07230 0.83 -0.1 1,324,197 - MMJJ_RS07230 0.88 -5.6 1,324,219 + +0.4 1,324,228 - -0.9 1,324,236 + -0.1 1,324,253 - -0.3 1,324,287 + +0.8 1,324,287 + -0.5 1,324,298 + -0.8 1,324,302 - -0.6 1,324,327 + +0.1 1,324,374 - +0.6 1,324,422 + -0.2 1,324,437 - +0.3 1,324,438 + +1.3 1,324,438 + +0.9 1,324,444 - -3.0 1,324,444 - +0.6 1,324,454 - +0.6 1,324,455 + +0.3 1,324,469 + +1.5 1,324,469 + -0.5 1,324,473 + +0.5 1,324,473 + -2.4 1,324,473 + +0.3 1,324,473 + +0.4 1,324,540 - MMJJ_RS07235 0.14 -0.7 1,324,540 - MMJJ_RS07235 0.14 -0.3 1,324,541 + MMJJ_RS07235 0.14 +0.2 1,324,639 - MMJJ_RS07235 0.22 +0.7 1,324,639 - MMJJ_RS07235 0.22 +0.2 1,324,639 - MMJJ_RS07235 0.22 +1.0 1,324,646 - MMJJ_RS07235 0.23 -0.2 1,324,647 + MMJJ_RS07235 0.23 -0.9 1,324,647 + MMJJ_RS07235 0.23 +1.7 1,324,647 + MMJJ_RS07235 0.23 -1.0 1,324,650 - MMJJ_RS07235 0.23 +0.7 1,324,650 - MMJJ_RS07235 0.23 -0.3 1,324,651 + MMJJ_RS07235 0.23 +1.3 1,324,659 - MMJJ_RS07235 0.24 -1.3 1,324,659 - -1.2 1,324,689 + MMJJ_RS07235 0.26 +0.8 1,324,785 + MMJJ_RS07235 0.34 +0.6 1,324,800 + MMJJ_RS07235 0.35 +0.6 1,324,828 - MMJJ_RS07235 0.37 -1.1 1,324,879 - MMJJ_RS07235 0.42 +0.7 1,324,880 + MMJJ_RS07235 0.42 -0.6 1,324,880 + MMJJ_RS07235 0.42 -0.0 1,324,882 - MMJJ_RS07235 0.42 -0.9 1,324,882 - MMJJ_RS07235 0.42 +0.8 1,324,882 - MMJJ_RS07235 0.42 -0.6 1,324,896 - MMJJ_RS07235 0.43 +0.1 1,324,896 - MMJJ_RS07235 0.43 +1.0 1,324,905 - MMJJ_RS07235 0.44 +0.5 1,324,906 + MMJJ_RS07235 0.44 -1.4 1,324,923 + MMJJ_RS07235 0.45 -3.1 1,324,952 - MMJJ_RS07235 0.47 +0.8 1,324,953 + MMJJ_RS07235 0.47 -0.3 1,324,961 + MMJJ_RS07235 0.48 +0.7 1,324,976 - MMJJ_RS07235 0.49 +0.9 1,324,976 - MMJJ_RS07235 0.49 +0.3 1,324,980 - MMJJ_RS07235 0.50 +0.7 1,324,981 + MMJJ_RS07235 0.50 -0.5 1,325,004 - MMJJ_RS07235 0.52 +1.3 1,325,004 - MMJJ_RS07235 0.52 -0.8 1,325,004 - MMJJ_RS07235 0.52 -0.3 1,325,005 + MMJJ_RS07235 0.52 +0.7 1,325,005 + MMJJ_RS07235 0.52 +1.7 1,325,005 + MMJJ_RS07235 0.52 -0.1 1,325,031 - MMJJ_RS07235 0.54 +0.1 1,325,038 + MMJJ_RS07235 0.54 -0.9 1,325,048 - MMJJ_RS07235 0.55 -1.9 1,325,049 + MMJJ_RS07235 0.55 +0.2 1,325,049 + MMJJ_RS07235 0.55 -1.6 1,325,049 + MMJJ_RS07235 0.55 -4.3 1,325,063 + MMJJ_RS07235 0.56 +0.5 1,325,070 + MMJJ_RS07235 0.57 +0.1 1,325,077 - MMJJ_RS07235 0.57 +0.4 1,325,077 - MMJJ_RS07235 0.57 +0.5 1,325,077 - MMJJ_RS07235 0.57 +0.1 1,325,078 + MMJJ_RS07235 0.58 -0.4 1,325,078 + MMJJ_RS07235 0.58 +0.0 1,325,078 + +5.2 1,325,078 + MMJJ_RS07235 0.58 -0.1 1,325,080 - MMJJ_RS07235 0.58 -0.4 1,325,166 + MMJJ_RS07235 0.65 -2.1 1,325,172 - MMJJ_RS07235 0.65 +0.9 1,325,172 - MMJJ_RS07235 0.65 +1.0 1,325,172 - MMJJ_RS07235 0.65 +1.1 1,325,173 + MMJJ_RS07235 0.65 +0.2 1,325,229 - MMJJ_RS07235 0.70 +0.2
Or see this region's nucleotide sequence