Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06535 and MMJJ_RS06540 are separated by 9 nucleotides MMJJ_RS06540 and MMJJ_RS06545 are separated by 26 nucleotides MMJJ_RS06545 and MMJJ_RS06550 are separated by 167 nucleotides
MMJJ_RS06535: MMJJ_RS06535 - UPF0104 family protein, at 1,205,911 to 1,206,933
_RS06535
MMJJ_RS06540: MMJJ_RS06540 - DUF2120 domain-containing protein, at 1,206,943 to 1,207,362
_RS06540
MMJJ_RS06545: MMJJ_RS06545 - hydrogenase nickel incorporation protein HypB, at 1,207,389 to 1,208,063
_RS06545
MMJJ_RS06550: MMJJ_RS06550 - ABC transporter permease, at 1,208,231 to 1,208,998
_RS06550
Position (kb)
1206
1207
1208 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1205.951 kb on - strand at 1205.951 kb on - strand at 1205.951 kb on - strand at 1205.951 kb on - strand at 1205.951 kb on - strand at 1205.951 kb on - strand at 1205.951 kb on - strand at 1205.952 kb on + strand at 1205.952 kb on + strand at 1205.952 kb on + strand at 1205.952 kb on + strand at 1205.952 kb on + strand at 1205.952 kb on + strand at 1205.965 kb on + strand at 1205.965 kb on + strand at 1205.972 kb on - strand at 1205.982 kb on - strand at 1205.989 kb on - strand at 1205.997 kb on + strand at 1206.046 kb on - strand, within MMJJ_RS06535 at 1206.046 kb on - strand, within MMJJ_RS06535 at 1206.068 kb on + strand, within MMJJ_RS06535 at 1206.068 kb on + strand, within MMJJ_RS06535 at 1206.071 kb on - strand, within MMJJ_RS06535 at 1206.072 kb on + strand, within MMJJ_RS06535 at 1206.072 kb on + strand, within MMJJ_RS06535 at 1206.082 kb on - strand, within MMJJ_RS06535 at 1206.082 kb on - strand, within MMJJ_RS06535 at 1206.107 kb on + strand, within MMJJ_RS06535 at 1206.188 kb on - strand, within MMJJ_RS06535 at 1206.232 kb on - strand, within MMJJ_RS06535 at 1206.232 kb on - strand, within MMJJ_RS06535 at 1206.232 kb on - strand, within MMJJ_RS06535 at 1206.304 kb on + strand, within MMJJ_RS06535 at 1206.309 kb on - strand, within MMJJ_RS06535 at 1206.309 kb on - strand, within MMJJ_RS06535 at 1206.336 kb on + strand, within MMJJ_RS06535 at 1206.339 kb on + strand, within MMJJ_RS06535 at 1206.339 kb on + strand, within MMJJ_RS06535 at 1206.342 kb on + strand, within MMJJ_RS06535 at 1206.385 kb on + strand, within MMJJ_RS06535 at 1206.411 kb on + strand, within MMJJ_RS06535 at 1206.421 kb on + strand, within MMJJ_RS06535 at 1206.421 kb on + strand, within MMJJ_RS06535 at 1206.427 kb on - strand, within MMJJ_RS06535 at 1206.427 kb on - strand, within MMJJ_RS06535 at 1206.427 kb on - strand, within MMJJ_RS06535 at 1206.427 kb on - strand, within MMJJ_RS06535 at 1206.427 kb on - strand, within MMJJ_RS06535 at 1206.427 kb on - strand, within MMJJ_RS06535 at 1206.428 kb on + strand, within MMJJ_RS06535 at 1206.428 kb on + strand, within MMJJ_RS06535 at 1206.428 kb on + strand, within MMJJ_RS06535 at 1206.428 kb on + strand, within MMJJ_RS06535 at 1206.428 kb on + strand, within MMJJ_RS06535 at 1206.428 kb on + strand, within MMJJ_RS06535 at 1206.428 kb on + strand, within MMJJ_RS06535 at 1206.462 kb on + strand, within MMJJ_RS06535 at 1206.502 kb on + strand, within MMJJ_RS06535 at 1206.509 kb on - strand, within MMJJ_RS06535 at 1206.531 kb on - strand at 1206.531 kb on - strand, within MMJJ_RS06535 at 1206.576 kb on - strand, within MMJJ_RS06535 at 1206.577 kb on + strand, within MMJJ_RS06535 at 1206.577 kb on + strand, within MMJJ_RS06535 at 1206.623 kb on - strand, within MMJJ_RS06535 at 1206.626 kb on - strand, within MMJJ_RS06535 at 1206.627 kb on + strand, within MMJJ_RS06535 at 1206.627 kb on + strand, within MMJJ_RS06535 at 1206.627 kb on + strand, within MMJJ_RS06535 at 1206.630 kb on - strand, within MMJJ_RS06535 at 1206.630 kb on - strand, within MMJJ_RS06535 at 1206.631 kb on + strand, within MMJJ_RS06535 at 1206.632 kb on - strand, within MMJJ_RS06535 at 1206.633 kb on + strand, within MMJJ_RS06535 at 1206.636 kb on - strand, within MMJJ_RS06535 at 1206.643 kb on - strand, within MMJJ_RS06535 at 1206.643 kb on - strand, within MMJJ_RS06535 at 1206.644 kb on + strand, within MMJJ_RS06535 at 1206.647 kb on - strand, within MMJJ_RS06535 at 1206.647 kb on - strand, within MMJJ_RS06535 at 1206.648 kb on + strand, within MMJJ_RS06535 at 1206.648 kb on + strand, within MMJJ_RS06535 at 1206.662 kb on - strand, within MMJJ_RS06535 at 1206.662 kb on - strand, within MMJJ_RS06535 at 1206.683 kb on - strand, within MMJJ_RS06535 at 1206.684 kb on + strand, within MMJJ_RS06535 at 1206.701 kb on + strand, within MMJJ_RS06535 at 1206.701 kb on + strand, within MMJJ_RS06535 at 1206.701 kb on + strand, within MMJJ_RS06535 at 1206.707 kb on - strand, within MMJJ_RS06535 at 1206.782 kb on + strand, within MMJJ_RS06535 at 1206.792 kb on - strand, within MMJJ_RS06535 at 1206.793 kb on + strand, within MMJJ_RS06535 at 1206.797 kb on - strand, within MMJJ_RS06535 at 1206.798 kb on + strand, within MMJJ_RS06535 at 1206.798 kb on + strand, within MMJJ_RS06535 at 1206.798 kb on + strand, within MMJJ_RS06535 at 1206.803 kb on + strand, within MMJJ_RS06535 at 1206.834 kb on - strand at 1206.834 kb on - strand at 1206.835 kb on + strand at 1206.835 kb on + strand at 1206.847 kb on - strand at 1206.848 kb on + strand at 1206.848 kb on + strand at 1206.848 kb on + strand at 1206.848 kb on + strand at 1206.890 kb on - strand at 1206.903 kb on - strand at 1206.916 kb on - strand at 1206.931 kb on - strand at 1206.931 kb on - strand at 1206.939 kb on - strand at 1206.943 kb on + strand at 1206.943 kb on + strand at 1206.943 kb on + strand at 1206.943 kb on + strand at 1206.949 kb on + strand at 1206.959 kb on - strand at 1206.960 kb on + strand at 1206.964 kb on + strand at 1206.996 kb on - strand, within MMJJ_RS06540 at 1207.017 kb on - strand, within MMJJ_RS06540 at 1207.147 kb on + strand, within MMJJ_RS06540 at 1207.169 kb on - strand, within MMJJ_RS06540 at 1207.291 kb on - strand, within MMJJ_RS06540 at 1207.303 kb on + strand, within MMJJ_RS06540 at 1207.313 kb on + strand, within MMJJ_RS06540 at 1207.350 kb on + strand at 1207.384 kb on - strand at 1207.404 kb on - strand at 1207.554 kb on + strand, within MMJJ_RS06545 at 1207.686 kb on - strand, within MMJJ_RS06545 at 1207.805 kb on - strand, within MMJJ_RS06545 at 1207.844 kb on + strand at 1208.010 kb on + strand at 1208.025 kb on - strand at 1208.025 kb on - strand at 1208.039 kb on - strand at 1208.076 kb on - strand at 1208.077 kb on + strand at 1208.077 kb on + strand at 1208.082 kb on - strand at 1208.082 kb on - strand at 1208.101 kb on + strand at 1208.102 kb on - strand at 1208.102 kb on - strand at 1208.102 kb on - strand at 1208.106 kb on - strand at 1208.106 kb on - strand at 1208.106 kb on - strand at 1208.133 kb on + strand at 1208.136 kb on - strand at 1208.139 kb on - strand at 1208.140 kb on + strand at 1208.140 kb on + strand at 1208.162 kb on - strand at 1208.165 kb on + strand at 1208.168 kb on - strand at 1208.169 kb on + strand at 1208.174 kb on - strand at 1208.179 kb on - strand at 1208.183 kb on + strand at 1208.183 kb on + strand at 1208.183 kb on + strand at 1208.183 kb on + strand at 1208.202 kb on - strand at 1208.212 kb on - strand at 1208.227 kb on - strand at 1208.277 kb on - strand at 1208.277 kb on - strand at 1208.289 kb on - strand at 1208.289 kb on - strand at 1208.290 kb on + strand at 1208.299 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 1,205,951 - +0.4 1,205,951 - -0.8 1,205,951 - -1.9 1,205,951 - -1.7 1,205,951 - +0.2 1,205,951 - -0.6 1,205,951 - -1.6 1,205,952 + +0.4 1,205,952 + -2.1 1,205,952 + +0.1 1,205,952 + -1.9 1,205,952 + -0.8 1,205,952 + +0.3 1,205,965 + -0.7 1,205,965 + -0.6 1,205,972 - +0.1 1,205,982 - -1.1 1,205,989 - -0.0 1,205,997 + -1.1 1,206,046 - MMJJ_RS06535 0.13 -0.5 1,206,046 - MMJJ_RS06535 0.13 -1.3 1,206,068 + MMJJ_RS06535 0.15 -2.4 1,206,068 + MMJJ_RS06535 0.15 -0.2 1,206,071 - MMJJ_RS06535 0.16 +0.4 1,206,072 + MMJJ_RS06535 0.16 -1.4 1,206,072 + MMJJ_RS06535 0.16 +0.3 1,206,082 - MMJJ_RS06535 0.17 +0.8 1,206,082 - MMJJ_RS06535 0.17 +0.7 1,206,107 + MMJJ_RS06535 0.19 +0.7 1,206,188 - MMJJ_RS06535 0.27 +0.5 1,206,232 - MMJJ_RS06535 0.31 -1.5 1,206,232 - MMJJ_RS06535 0.31 -0.9 1,206,232 - MMJJ_RS06535 0.31 -0.9 1,206,304 + MMJJ_RS06535 0.38 +1.5 1,206,309 - MMJJ_RS06535 0.39 +1.2 1,206,309 - MMJJ_RS06535 0.39 -0.2 1,206,336 + MMJJ_RS06535 0.42 -0.9 1,206,339 + MMJJ_RS06535 0.42 -2.6 1,206,339 + MMJJ_RS06535 0.42 -2.7 1,206,342 + MMJJ_RS06535 0.42 +0.6 1,206,385 + MMJJ_RS06535 0.46 -0.6 1,206,411 + MMJJ_RS06535 0.49 +0.5 1,206,421 + MMJJ_RS06535 0.50 -0.4 1,206,421 + MMJJ_RS06535 0.50 +0.0 1,206,427 - MMJJ_RS06535 0.50 +0.5 1,206,427 - MMJJ_RS06535 0.50 -3.7 1,206,427 - MMJJ_RS06535 0.50 +0.7 1,206,427 - MMJJ_RS06535 0.50 +1.4 1,206,427 - MMJJ_RS06535 0.50 -0.2 1,206,427 - MMJJ_RS06535 0.50 -0.2 1,206,428 + MMJJ_RS06535 0.51 +0.2 1,206,428 + MMJJ_RS06535 0.51 +0.3 1,206,428 + MMJJ_RS06535 0.51 +0.0 1,206,428 + MMJJ_RS06535 0.51 +0.6 1,206,428 + MMJJ_RS06535 0.51 -0.1 1,206,428 + MMJJ_RS06535 0.51 -0.3 1,206,428 + MMJJ_RS06535 0.51 -0.3 1,206,462 + MMJJ_RS06535 0.54 -0.1 1,206,502 + MMJJ_RS06535 0.58 -0.9 1,206,509 - MMJJ_RS06535 0.58 -0.9 1,206,531 - -0.6 1,206,531 - MMJJ_RS06535 0.61 -0.1 1,206,576 - MMJJ_RS06535 0.65 -2.0 1,206,577 + MMJJ_RS06535 0.65 -0.6 1,206,577 + MMJJ_RS06535 0.65 +0.1 1,206,623 - MMJJ_RS06535 0.70 -0.6 1,206,626 - MMJJ_RS06535 0.70 +0.0 1,206,627 + MMJJ_RS06535 0.70 -0.9 1,206,627 + MMJJ_RS06535 0.70 -1.5 1,206,627 + MMJJ_RS06535 0.70 -1.6 1,206,630 - MMJJ_RS06535 0.70 +0.3 1,206,630 - MMJJ_RS06535 0.70 -3.2 1,206,631 + MMJJ_RS06535 0.70 -0.0 1,206,632 - MMJJ_RS06535 0.70 -0.3 1,206,633 + MMJJ_RS06535 0.71 -0.4 1,206,636 - MMJJ_RS06535 0.71 -0.4 1,206,643 - MMJJ_RS06535 0.72 -0.6 1,206,643 - MMJJ_RS06535 0.72 -0.3 1,206,644 + MMJJ_RS06535 0.72 -0.7 1,206,647 - MMJJ_RS06535 0.72 +0.2 1,206,647 - MMJJ_RS06535 0.72 +0.5 1,206,648 + MMJJ_RS06535 0.72 -2.3 1,206,648 + MMJJ_RS06535 0.72 -0.1 1,206,662 - MMJJ_RS06535 0.73 +0.2 1,206,662 - MMJJ_RS06535 0.73 -0.4 1,206,683 - MMJJ_RS06535 0.75 -0.1 1,206,684 + MMJJ_RS06535 0.76 -4.4 1,206,701 + MMJJ_RS06535 0.77 -0.4 1,206,701 + MMJJ_RS06535 0.77 -1.6 1,206,701 + MMJJ_RS06535 0.77 +0.2 1,206,707 - MMJJ_RS06535 0.78 +0.3 1,206,782 + MMJJ_RS06535 0.85 +0.3 1,206,792 - MMJJ_RS06535 0.86 +0.4 1,206,793 + MMJJ_RS06535 0.86 +0.1 1,206,797 - MMJJ_RS06535 0.87 +0.1 1,206,798 + MMJJ_RS06535 0.87 -1.0 1,206,798 + MMJJ_RS06535 0.87 -3.8 1,206,798 + MMJJ_RS06535 0.87 +0.3 1,206,803 + MMJJ_RS06535 0.87 -0.7 1,206,834 - -0.4 1,206,834 - -0.3 1,206,835 + -0.9 1,206,835 + -0.4 1,206,847 - +0.3 1,206,848 + +0.9 1,206,848 + -0.6 1,206,848 + -1.0 1,206,848 + -4.9 1,206,890 - -0.0 1,206,903 - -0.0 1,206,916 - -0.5 1,206,931 - +0.3 1,206,931 - -1.4 1,206,939 - +1.0 1,206,943 + -0.6 1,206,943 + -4.4 1,206,943 + +1.1 1,206,943 + +0.1 1,206,949 + +0.4 1,206,959 - +0.2 1,206,960 + +1.2 1,206,964 + +0.4 1,206,996 - MMJJ_RS06540 0.13 +0.7 1,207,017 - MMJJ_RS06540 0.18 +0.1 1,207,147 + MMJJ_RS06540 0.49 +0.8 1,207,169 - MMJJ_RS06540 0.54 +0.8 1,207,291 - MMJJ_RS06540 0.83 +0.6 1,207,303 + MMJJ_RS06540 0.86 -0.4 1,207,313 + MMJJ_RS06540 0.88 -3.2 1,207,350 + +0.2 1,207,384 - +0.5 1,207,404 - +0.5 1,207,554 + MMJJ_RS06545 0.24 -2.1 1,207,686 - MMJJ_RS06545 0.44 +1.0 1,207,805 - MMJJ_RS06545 0.62 -1.5 1,207,844 + -1.0 1,208,010 + +0.6 1,208,025 - -1.6 1,208,025 - -0.1 1,208,039 - +1.0 1,208,076 - +1.1 1,208,077 + +1.3 1,208,077 + -1.8 1,208,082 - +1.9 1,208,082 - +0.6 1,208,101 + -1.4 1,208,102 - -1.1 1,208,102 - +0.8 1,208,102 - +0.7 1,208,106 - +0.8 1,208,106 - +0.4 1,208,106 - +0.7 1,208,133 + -0.5 1,208,136 - +1.3 1,208,139 - -0.1 1,208,140 + -0.0 1,208,140 + -0.3 1,208,162 - +0.2 1,208,165 + +0.2 1,208,168 - +0.8 1,208,169 + -2.8 1,208,174 - +0.6 1,208,179 - -0.1 1,208,183 + +0.2 1,208,183 + +0.4 1,208,183 + +0.4 1,208,183 + +0.6 1,208,202 - +0.8 1,208,212 - +0.1 1,208,227 - -0.6 1,208,277 - +1.1 1,208,277 - +0.0 1,208,289 - -0.3 1,208,289 - +0.6 1,208,290 + -2.8 1,208,299 + +0.3
Or see this region's nucleotide sequence