Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06490 and MMJJ_RS06495 are separated by 9 nucleotides MMJJ_RS06495 and MMJJ_RS06500 overlap by 4 nucleotides MMJJ_RS06500 and MMJJ_RS06505 overlap by 4 nucleotides
MMJJ_RS06490: MMJJ_RS06490 - methanogenesis marker 2 protein, at 1,197,534 to 1,198,505
_RS06490
MMJJ_RS06495: MMJJ_RS06495 - hypothetical protein, at 1,198,515 to 1,198,805
_RS06495
MMJJ_RS06500: MMJJ_RS06500 - class I SAM-dependent methyltransferase, at 1,198,802 to 1,199,512
_RS06500
MMJJ_RS06505: MMJJ_RS06505 - prephenate dehydratase, at 1,199,509 to 1,200,315
_RS06505
Position (kb)
1198
1199 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1197.647 kb on - strand, within MMJJ_RS06490 at 1197.656 kb on + strand, within MMJJ_RS06490 at 1197.836 kb on - strand, within MMJJ_RS06490 at 1197.847 kb on - strand, within MMJJ_RS06490 at 1197.962 kb on + strand at 1198.119 kb on - strand at 1198.181 kb on - strand, within MMJJ_RS06490 at 1198.189 kb on - strand, within MMJJ_RS06490 at 1198.265 kb on - strand at 1198.333 kb on - strand, within MMJJ_RS06490 at 1198.482 kb on + strand at 1198.488 kb on - strand at 1198.488 kb on - strand at 1198.488 kb on - strand at 1198.489 kb on + strand at 1198.489 kb on + strand at 1198.528 kb on - strand at 1198.528 kb on - strand at 1198.528 kb on - strand at 1198.528 kb on - strand at 1198.528 kb on - strand at 1198.528 kb on - strand at 1198.529 kb on + strand at 1198.538 kb on + strand at 1198.542 kb on - strand at 1198.559 kb on + strand, within MMJJ_RS06495 at 1198.665 kb on - strand, within MMJJ_RS06495 at 1198.711 kb on - strand, within MMJJ_RS06495 at 1198.726 kb on - strand, within MMJJ_RS06495 at 1198.751 kb on + strand, within MMJJ_RS06495 at 1198.772 kb on - strand, within MMJJ_RS06495 at 1198.787 kb on - strand at 1198.787 kb on - strand at 1198.827 kb on - strand at 1198.863 kb on + strand at 1198.868 kb on + strand at 1198.875 kb on - strand, within MMJJ_RS06500 at 1198.876 kb on + strand, within MMJJ_RS06500 at 1198.885 kb on + strand, within MMJJ_RS06500 at 1198.967 kb on + strand, within MMJJ_RS06500 at 1198.969 kb on - strand, within MMJJ_RS06500 at 1198.969 kb on - strand, within MMJJ_RS06500 at 1198.975 kb on + strand, within MMJJ_RS06500 at 1199.007 kb on + strand, within MMJJ_RS06500 at 1199.014 kb on + strand, within MMJJ_RS06500 at 1199.014 kb on + strand, within MMJJ_RS06500 at 1199.030 kb on + strand, within MMJJ_RS06500 at 1199.036 kb on - strand, within MMJJ_RS06500 at 1199.037 kb on + strand, within MMJJ_RS06500 at 1199.072 kb on + strand, within MMJJ_RS06500 at 1199.119 kb on - strand, within MMJJ_RS06500 at 1199.119 kb on - strand, within MMJJ_RS06500 at 1199.119 kb on - strand, within MMJJ_RS06500 at 1199.120 kb on + strand, within MMJJ_RS06500 at 1199.228 kb on - strand, within MMJJ_RS06500 at 1199.239 kb on + strand, within MMJJ_RS06500 at 1199.241 kb on - strand, within MMJJ_RS06500 at 1199.242 kb on + strand, within MMJJ_RS06500 at 1199.408 kb on + strand, within MMJJ_RS06500 at 1199.412 kb on - strand, within MMJJ_RS06500
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 1,197,647 - MMJJ_RS06490 0.12 -2.9 1,197,656 + MMJJ_RS06490 0.13 +1.2 1,197,836 - MMJJ_RS06490 0.31 +0.6 1,197,847 - MMJJ_RS06490 0.32 -0.4 1,197,962 + -0.6 1,198,119 - +1.2 1,198,181 - MMJJ_RS06490 0.67 +2.2 1,198,189 - MMJJ_RS06490 0.67 -0.6 1,198,265 - +3.4 1,198,333 - MMJJ_RS06490 0.82 -1.0 1,198,482 + -2.5 1,198,488 - -0.0 1,198,488 - -5.0 1,198,488 - -0.4 1,198,489 + -5.0 1,198,489 + -3.1 1,198,528 - +0.5 1,198,528 - +1.5 1,198,528 - +0.3 1,198,528 - +0.3 1,198,528 - +0.6 1,198,528 - +1.0 1,198,529 + +0.9 1,198,538 + +0.1 1,198,542 - +0.8 1,198,559 + MMJJ_RS06495 0.15 -1.0 1,198,665 - MMJJ_RS06495 0.52 +1.8 1,198,711 - MMJJ_RS06495 0.67 +0.2 1,198,726 - MMJJ_RS06495 0.73 +0.2 1,198,751 + MMJJ_RS06495 0.81 +0.7 1,198,772 - MMJJ_RS06495 0.88 -0.3 1,198,787 - +0.4 1,198,787 - +0.5 1,198,827 - -0.6 1,198,863 + +0.2 1,198,868 + +0.8 1,198,875 - MMJJ_RS06500 0.10 +0.4 1,198,876 + MMJJ_RS06500 0.10 -0.8 1,198,885 + MMJJ_RS06500 0.12 +0.4 1,198,967 + MMJJ_RS06500 0.23 -0.5 1,198,969 - MMJJ_RS06500 0.23 +0.5 1,198,969 - MMJJ_RS06500 0.23 -0.5 1,198,975 + MMJJ_RS06500 0.24 +0.4 1,199,007 + MMJJ_RS06500 0.29 -2.6 1,199,014 + MMJJ_RS06500 0.30 +0.8 1,199,014 + MMJJ_RS06500 0.30 +0.5 1,199,030 + MMJJ_RS06500 0.32 +0.1 1,199,036 - MMJJ_RS06500 0.33 -0.1 1,199,037 + MMJJ_RS06500 0.33 +0.3 1,199,072 + MMJJ_RS06500 0.38 -5.4 1,199,119 - MMJJ_RS06500 0.45 +0.9 1,199,119 - MMJJ_RS06500 0.45 -0.4 1,199,119 - MMJJ_RS06500 0.45 -1.1 1,199,120 + MMJJ_RS06500 0.45 +0.4 1,199,228 - MMJJ_RS06500 0.60 +0.4 1,199,239 + MMJJ_RS06500 0.61 +0.4 1,199,241 - MMJJ_RS06500 0.62 -1.5 1,199,242 + MMJJ_RS06500 0.62 -1.9 1,199,408 + MMJJ_RS06500 0.85 -2.1 1,199,412 - MMJJ_RS06500 0.86 +0.6
Or see this region's nucleotide sequence