Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06335 and MMJJ_RS06340 are separated by 267 nucleotides MMJJ_RS06340 and MMJJ_RS06345 are separated by 47 nucleotides MMJJ_RS06345 and MMJJ_RS06350 are separated by 9 nucleotides
MMJJ_RS06335: MMJJ_RS06335 - tetrahydromethanopterin S-methyltransferase subunit E, at 1,172,753 to 1,173,652
_RS06335
MMJJ_RS06340: MMJJ_RS06340 - coenzyme-B sulfoethylthiotransferase subunit alpha, at 1,173,920 to 1,175,581
_RS06340
MMJJ_RS06345: MMJJ_RS06345 - coenzyme-B sulfoethylthiotransferase subunit gamma, at 1,175,629 to 1,176,411
_RS06345
MMJJ_RS06350: MMJJ_RS06350 - methyl-coenzyme M reductase I operon protein C, at 1,176,421 to 1,177,017
_RS06350
Position (kb)
1173
1174
1175
1176 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1172.939 kb on - strand, within MMJJ_RS06335 at 1172.948 kb on + strand, within MMJJ_RS06335 at 1173.451 kb on + strand at 1173.716 kb on - strand at 1173.722 kb on - strand at 1173.762 kb on - strand at 1173.763 kb on + strand at 1173.763 kb on + strand at 1173.845 kb on + strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.874 kb on - strand at 1173.875 kb on + strand at 1173.875 kb on + strand at 1173.875 kb on + strand at 1173.875 kb on + strand at 1173.875 kb on + strand at 1173.878 kb on - strand at 1173.879 kb on + strand at 1173.880 kb on - strand at 1173.933 kb on + strand at 1174.331 kb on + strand, within MMJJ_RS06340 at 1174.334 kb on - strand at 1174.335 kb on + strand, within MMJJ_RS06340 at 1174.420 kb on + strand, within MMJJ_RS06340 at 1174.527 kb on - strand, within MMJJ_RS06340 at 1175.246 kb on + strand at 1176.067 kb on - strand at 1176.107 kb on - strand, within MMJJ_RS06345 at 1176.129 kb on + strand, within MMJJ_RS06345 at 1176.335 kb on - strand at 1176.405 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 1,172,939 - MMJJ_RS06335 0.21 -0.8 1,172,948 + MMJJ_RS06335 0.22 -0.6 1,173,451 + +0.6 1,173,716 - -1.5 1,173,722 - +0.2 1,173,762 - -0.4 1,173,763 + -1.4 1,173,763 + -0.4 1,173,845 + -0.8 1,173,874 - -0.7 1,173,874 - -0.3 1,173,874 - +0.1 1,173,874 - -1.0 1,173,874 - -1.1 1,173,874 - +0.5 1,173,874 - -0.8 1,173,874 - +2.6 1,173,875 + -0.8 1,173,875 + -0.2 1,173,875 + +0.6 1,173,875 + -1.8 1,173,875 + -0.1 1,173,878 - +0.6 1,173,879 + +1.4 1,173,880 - -0.1 1,173,933 + +1.3 1,174,331 + MMJJ_RS06340 0.25 +0.1 1,174,334 - +0.6 1,174,335 + MMJJ_RS06340 0.25 -0.4 1,174,420 + MMJJ_RS06340 0.30 +2.0 1,174,527 - MMJJ_RS06340 0.37 -0.5 1,175,246 + +0.6 1,176,067 - +0.6 1,176,107 - MMJJ_RS06345 0.61 -0.6 1,176,129 + MMJJ_RS06345 0.64 -0.6 1,176,335 - +0.6 1,176,405 + +4.2
Or see this region's nucleotide sequence