Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05835 and MMJJ_RS05840 are separated by 122 nucleotides MMJJ_RS05840 and MMJJ_RS05845 are separated by 116 nucleotides MMJJ_RS05845 and MMJJ_RS05850 are separated by 38 nucleotides MMJJ_RS05850 and MMJJ_RS05855 are separated by 14 nucleotides MMJJ_RS05855 and MMJJ_RS05860 are separated by 13 nucleotides MMJJ_RS05860 and MMJJ_RS05865 are separated by 29 nucleotides MMJJ_RS05865 and MMJJ_RS05870 are separated by 114 nucleotides MMJJ_RS05870 and MMJJ_RS05875 are separated by 1 nucleotides
MMJJ_RS05835: MMJJ_RS05835 - bis-aminopropyl spermidine synthase family protein, at 1,086,013 to 1,087,185
_RS05835
MMJJ_RS05840: MMJJ_RS05840 - tRNA-Phe, at 1,087,308 to 1,087,381
_RS05840
MMJJ_RS05845: MMJJ_RS05845 - tRNA-Asn, at 1,087,498 to 1,087,574
_RS05845
MMJJ_RS05850: MMJJ_RS05850 - tRNA-Ile, at 1,087,613 to 1,087,687
_RS05850
MMJJ_RS05855: MMJJ_RS05855 - tRNA-Glu, at 1,087,702 to 1,087,776
_RS05855
MMJJ_RS05860: MMJJ_RS05860 - tRNA-Leu, at 1,087,790 to 1,087,874
_RS05860
MMJJ_RS05865: MMJJ_RS05865 - tRNA-His, at 1,087,904 to 1,087,978
_RS05865
MMJJ_RS05870: MMJJ_RS05870 - pyridoxal 5'-phosphate synthase glutaminase subunit PdxT, at 1,088,093 to 1,088,656
_RS05870
MMJJ_RS05875: MMJJ_RS05875 - hypothetical protein, at 1,088,658 to 1,091,747
_RS05875
Position (kb)
1088
1089 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1087.199 kb on - strand at 1087.199 kb on - strand at 1087.199 kb on - strand at 1087.201 kb on - strand at 1087.201 kb on - strand at 1087.202 kb on + strand at 1087.214 kb on - strand at 1087.217 kb on - strand at 1087.217 kb on - strand at 1087.220 kb on - strand at 1087.220 kb on - strand at 1087.220 kb on - strand at 1087.220 kb on - strand at 1087.225 kb on - strand at 1087.226 kb on + strand at 1087.226 kb on + strand at 1087.226 kb on + strand at 1087.231 kb on - strand at 1087.238 kb on + strand at 1087.242 kb on - strand at 1087.243 kb on + strand at 1087.243 kb on + strand at 1087.253 kb on + strand at 1087.253 kb on + strand at 1088.052 kb on + strand at 1088.200 kb on + strand, within MMJJ_RS05870 at 1088.217 kb on - strand, within MMJJ_RS05870 at 1088.221 kb on + strand, within MMJJ_RS05870 at 1088.240 kb on + strand, within MMJJ_RS05870 at 1088.263 kb on + strand, within MMJJ_RS05870 at 1088.263 kb on + strand, within MMJJ_RS05870 at 1088.338 kb on + strand, within MMJJ_RS05870 at 1088.367 kb on - strand, within MMJJ_RS05870 at 1088.405 kb on - strand, within MMJJ_RS05870 at 1088.406 kb on + strand, within MMJJ_RS05870 at 1088.406 kb on + strand, within MMJJ_RS05870 at 1088.547 kb on - strand, within MMJJ_RS05870 at 1088.553 kb on - strand, within MMJJ_RS05870 at 1088.619 kb on + strand at 1088.638 kb on - strand at 1088.639 kb on + strand at 1088.643 kb on - strand at 1088.643 kb on - strand at 1088.644 kb on + strand at 1088.660 kb on + strand at 1088.663 kb on + strand at 1088.663 kb on + strand at 1088.670 kb on + strand at 1088.712 kb on - strand at 1088.712 kb on - strand at 1088.712 kb on - strand at 1088.713 kb on + strand at 1088.713 kb on + strand at 1088.713 kb on + strand at 1088.717 kb on + strand at 1088.757 kb on - strand at 1088.758 kb on + strand at 1088.761 kb on - strand at 1088.762 kb on + strand at 1088.762 kb on + strand at 1088.770 kb on + strand at 1088.792 kb on - strand at 1088.793 kb on + strand at 1088.843 kb on + strand at 1088.843 kb on + strand at 1088.843 kb on + strand at 1088.849 kb on - strand at 1088.850 kb on + strand at 1088.905 kb on - strand at 1088.910 kb on - strand at 1088.911 kb on + strand at 1088.955 kb on - strand at 1088.955 kb on - strand at 1088.955 kb on - strand at 1088.956 kb on + strand at 1088.965 kb on + strand at 1089.003 kb on + strand, within MMJJ_RS05875 at 1089.086 kb on - strand, within MMJJ_RS05875 at 1089.097 kb on - strand, within MMJJ_RS05875 at 1089.097 kb on - strand, within MMJJ_RS05875 at 1089.097 kb on - strand, within MMJJ_RS05875 at 1089.097 kb on - strand, within MMJJ_RS05875 at 1089.097 kb on - strand, within MMJJ_RS05875 at 1089.098 kb on + strand, within MMJJ_RS05875 at 1089.121 kb on + strand, within MMJJ_RS05875 at 1089.153 kb on + strand, within MMJJ_RS05875 at 1089.157 kb on + strand, within MMJJ_RS05875 at 1089.157 kb on + strand, within MMJJ_RS05875 at 1089.186 kb on + strand, within MMJJ_RS05875 at 1089.188 kb on + strand, within MMJJ_RS05875 at 1089.196 kb on + strand, within MMJJ_RS05875 at 1089.236 kb on - strand, within MMJJ_RS05875 at 1089.236 kb on - strand, within MMJJ_RS05875 at 1089.237 kb on + strand, within MMJJ_RS05875 at 1089.240 kb on - strand, within MMJJ_RS05875 at 1089.241 kb on + strand, within MMJJ_RS05875 at 1089.268 kb on - strand at 1089.269 kb on + strand, within MMJJ_RS05875 at 1089.269 kb on + strand, within MMJJ_RS05875 at 1089.269 kb on + strand, within MMJJ_RS05875 at 1089.278 kb on - strand, within MMJJ_RS05875 at 1089.278 kb on - strand, within MMJJ_RS05875 at 1089.278 kb on - strand, within MMJJ_RS05875 at 1089.279 kb on + strand, within MMJJ_RS05875 at 1089.279 kb on + strand, within MMJJ_RS05875 at 1089.279 kb on + strand, within MMJJ_RS05875 at 1089.279 kb on + strand, within MMJJ_RS05875 at 1089.332 kb on - strand, within MMJJ_RS05875 at 1089.332 kb on - strand, within MMJJ_RS05875 at 1089.332 kb on - strand, within MMJJ_RS05875 at 1089.337 kb on + strand, within MMJJ_RS05875 at 1089.356 kb on - strand, within MMJJ_RS05875 at 1089.357 kb on + strand, within MMJJ_RS05875 at 1089.357 kb on + strand, within MMJJ_RS05875 at 1089.365 kb on - strand, within MMJJ_RS05875 at 1089.366 kb on + strand, within MMJJ_RS05875 at 1089.400 kb on - strand, within MMJJ_RS05875 at 1089.413 kb on - strand, within MMJJ_RS05875 at 1089.413 kb on - strand, within MMJJ_RS05875 at 1089.413 kb on - strand, within MMJJ_RS05875 at 1089.415 kb on - strand, within MMJJ_RS05875 at 1089.416 kb on + strand, within MMJJ_RS05875 at 1089.433 kb on - strand, within MMJJ_RS05875 at 1089.433 kb on - strand, within MMJJ_RS05875 at 1089.438 kb on - strand, within MMJJ_RS05875 at 1089.439 kb on + strand, within MMJJ_RS05875 at 1089.513 kb on + strand, within MMJJ_RS05875 at 1089.514 kb on - strand, within MMJJ_RS05875 at 1089.514 kb on - strand, within MMJJ_RS05875 at 1089.515 kb on + strand, within MMJJ_RS05875 at 1089.515 kb on + strand, within MMJJ_RS05875 at 1089.579 kb on - strand, within MMJJ_RS05875 at 1089.579 kb on - strand, within MMJJ_RS05875 at 1089.591 kb on - strand, within MMJJ_RS05875 at 1089.591 kb on - strand, within MMJJ_RS05875 at 1089.591 kb on - strand, within MMJJ_RS05875 at 1089.591 kb on - strand, within MMJJ_RS05875 at 1089.592 kb on + strand, within MMJJ_RS05875 at 1089.624 kb on - strand, within MMJJ_RS05875 at 1089.625 kb on + strand, within MMJJ_RS05875
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 1,087,199 - -1.5 1,087,199 - +0.7 1,087,199 - +0.5 1,087,201 - -0.5 1,087,201 - +0.6 1,087,202 + -0.5 1,087,214 - -0.3 1,087,217 - -0.1 1,087,217 - -0.5 1,087,220 - +0.7 1,087,220 - -1.6 1,087,220 - +0.3 1,087,220 - -1.7 1,087,225 - +0.6 1,087,226 + -0.2 1,087,226 + +0.0 1,087,226 + +0.9 1,087,231 - -0.3 1,087,238 + -3.8 1,087,242 - -1.0 1,087,243 + -0.3 1,087,243 + +0.6 1,087,253 + -0.7 1,087,253 + +0.7 1,088,052 + +0.3 1,088,200 + MMJJ_RS05870 0.19 +0.9 1,088,217 - MMJJ_RS05870 0.22 -3.4 1,088,221 + MMJJ_RS05870 0.23 +1.2 1,088,240 + MMJJ_RS05870 0.26 +1.1 1,088,263 + MMJJ_RS05870 0.30 +1.3 1,088,263 + MMJJ_RS05870 0.30 +1.8 1,088,338 + MMJJ_RS05870 0.43 -2.1 1,088,367 - MMJJ_RS05870 0.49 -3.7 1,088,405 - MMJJ_RS05870 0.55 -1.1 1,088,406 + MMJJ_RS05870 0.55 -0.4 1,088,406 + MMJJ_RS05870 0.55 +0.7 1,088,547 - MMJJ_RS05870 0.80 +0.4 1,088,553 - MMJJ_RS05870 0.82 +0.8 1,088,619 + +0.1 1,088,638 - +0.4 1,088,639 + +0.2 1,088,643 - -0.3 1,088,643 - +0.8 1,088,644 + -0.3 1,088,660 + -0.3 1,088,663 + -0.5 1,088,663 + +0.6 1,088,670 + -0.3 1,088,712 - +0.1 1,088,712 - +0.4 1,088,712 - +0.0 1,088,713 + -0.7 1,088,713 + +0.0 1,088,713 + -0.1 1,088,717 + -0.2 1,088,757 - -2.5 1,088,758 + +0.6 1,088,761 - -0.8 1,088,762 + -0.2 1,088,762 + -0.0 1,088,770 + +0.1 1,088,792 - -1.5 1,088,793 + +0.0 1,088,843 + -0.7 1,088,843 + -0.8 1,088,843 + +0.5 1,088,849 - -1.2 1,088,850 + +0.1 1,088,905 - +0.1 1,088,910 - -0.3 1,088,911 + +0.1 1,088,955 - +0.4 1,088,955 - +0.3 1,088,955 - -0.1 1,088,956 + -0.3 1,088,965 + -5.2 1,089,003 + MMJJ_RS05875 0.11 +0.8 1,089,086 - MMJJ_RS05875 0.14 +0.2 1,089,097 - MMJJ_RS05875 0.14 -0.3 1,089,097 - MMJJ_RS05875 0.14 +0.2 1,089,097 - MMJJ_RS05875 0.14 +1.1 1,089,097 - MMJJ_RS05875 0.14 -0.7 1,089,097 - MMJJ_RS05875 0.14 -1.6 1,089,098 + MMJJ_RS05875 0.14 +0.8 1,089,121 + MMJJ_RS05875 0.15 +0.3 1,089,153 + MMJJ_RS05875 0.16 +0.8 1,089,157 + MMJJ_RS05875 0.16 -0.6 1,089,157 + MMJJ_RS05875 0.16 +0.9 1,089,186 + MMJJ_RS05875 0.17 -2.5 1,089,188 + MMJJ_RS05875 0.17 -0.4 1,089,196 + MMJJ_RS05875 0.17 +0.5 1,089,236 - MMJJ_RS05875 0.19 -0.1 1,089,236 - MMJJ_RS05875 0.19 -0.9 1,089,237 + MMJJ_RS05875 0.19 -0.8 1,089,240 - MMJJ_RS05875 0.19 +0.4 1,089,241 + MMJJ_RS05875 0.19 -0.1 1,089,268 - -1.0 1,089,269 + MMJJ_RS05875 0.20 +0.0 1,089,269 + MMJJ_RS05875 0.20 -1.2 1,089,269 + MMJJ_RS05875 0.20 +0.8 1,089,278 - MMJJ_RS05875 0.20 +0.1 1,089,278 - MMJJ_RS05875 0.20 -0.0 1,089,278 - MMJJ_RS05875 0.20 -1.9 1,089,279 + MMJJ_RS05875 0.20 +0.1 1,089,279 + MMJJ_RS05875 0.20 -0.5 1,089,279 + MMJJ_RS05875 0.20 +0.8 1,089,279 + MMJJ_RS05875 0.20 -2.4 1,089,332 - MMJJ_RS05875 0.22 -2.0 1,089,332 - MMJJ_RS05875 0.22 +0.6 1,089,332 - MMJJ_RS05875 0.22 +0.2 1,089,337 + MMJJ_RS05875 0.22 +0.7 1,089,356 - MMJJ_RS05875 0.23 -0.3 1,089,357 + MMJJ_RS05875 0.23 -3.0 1,089,357 + MMJJ_RS05875 0.23 +0.1 1,089,365 - MMJJ_RS05875 0.23 -0.2 1,089,366 + MMJJ_RS05875 0.23 +0.4 1,089,400 - MMJJ_RS05875 0.24 +0.5 1,089,413 - MMJJ_RS05875 0.24 +0.7 1,089,413 - MMJJ_RS05875 0.24 +0.5 1,089,413 - MMJJ_RS05875 0.24 -0.2 1,089,415 - MMJJ_RS05875 0.24 -0.2 1,089,416 + MMJJ_RS05875 0.25 +0.7 1,089,433 - MMJJ_RS05875 0.25 +0.2 1,089,433 - MMJJ_RS05875 0.25 -0.1 1,089,438 - MMJJ_RS05875 0.25 +0.7 1,089,439 + MMJJ_RS05875 0.25 +0.1 1,089,513 + MMJJ_RS05875 0.28 -1.8 1,089,514 - MMJJ_RS05875 0.28 +0.5 1,089,514 - MMJJ_RS05875 0.28 +0.0 1,089,515 + MMJJ_RS05875 0.28 +0.5 1,089,515 + MMJJ_RS05875 0.28 +1.6 1,089,579 - MMJJ_RS05875 0.30 +1.1 1,089,579 - MMJJ_RS05875 0.30 -0.2 1,089,591 - MMJJ_RS05875 0.30 +1.6 1,089,591 - MMJJ_RS05875 0.30 -0.2 1,089,591 - MMJJ_RS05875 0.30 -0.6 1,089,591 - MMJJ_RS05875 0.30 +1.2 1,089,592 + MMJJ_RS05875 0.30 -0.7 1,089,624 - MMJJ_RS05875 0.31 -0.1 1,089,625 + MMJJ_RS05875 0.31 -4.3
Or see this region's nucleotide sequence