Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05545 and MMJJ_RS05550 are separated by 33 nucleotides MMJJ_RS05550 and MMJJ_RS05555 overlap by 44 nucleotides MMJJ_RS05555 and MMJJ_RS05560 are separated by 22 nucleotides MMJJ_RS05560 and MMJJ_RS05565 are separated by 113 nucleotides MMJJ_RS05565 and MMJJ_RS05570 are separated by 8 nucleotides
MMJJ_RS05545: MMJJ_RS05545 - DNA polymerase sliding clamp, at 1,037,683 to 1,038,435
_RS05545
MMJJ_RS05550: MMJJ_RS05550 - hypothetical protein, at 1,038,469 to 1,039,056
_RS05550
MMJJ_RS05555: MMJJ_RS05555 - 50S ribosomal protein L44e, at 1,039,013 to 1,039,294
_RS05555
MMJJ_RS05560: MMJJ_RS05560 - 30S ribosomal protein S27e, at 1,039,317 to 1,039,502
_RS05560
MMJJ_RS05565: MMJJ_RS05565 - translation initiation factor IF-2 subunit alpha, at 1,039,616 to 1,040,413
_RS05565
MMJJ_RS05570: MMJJ_RS05570 - RNA-protein complex protein Nop10, at 1,040,422 to 1,040,577
_RS05570
Position (kb)
1039
1040 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1038.473 kb on + strand at 1038.482 kb on - strand at 1039.292 kb on - strand at 1039.292 kb on - strand at 1039.293 kb on + strand at 1039.325 kb on + strand at 1039.337 kb on - strand, within MMJJ_RS05560 at 1039.338 kb on + strand, within MMJJ_RS05560 at 1039.388 kb on - strand, within MMJJ_RS05560 at 1039.388 kb on - strand, within MMJJ_RS05560 at 1039.389 kb on + strand, within MMJJ_RS05560 at 1039.416 kb on + strand, within MMJJ_RS05560 at 1039.487 kb on + strand at 1039.492 kb on - strand at 1039.492 kb on - strand at 1039.493 kb on + strand at 1039.493 kb on + strand at 1039.495 kb on - strand at 1039.496 kb on + strand at 1039.500 kb on - strand at 1039.511 kb on + strand at 1039.520 kb on - strand at 1039.520 kb on - strand at 1039.547 kb on - strand at 1039.547 kb on - strand at 1039.550 kb on + strand at 1039.557 kb on - strand at 1039.558 kb on + strand at 1039.571 kb on - strand at 1039.572 kb on + strand at 1039.594 kb on + strand at 1039.648 kb on - strand at 1039.765 kb on - strand, within MMJJ_RS05565 at 1039.767 kb on - strand, within MMJJ_RS05565 at 1039.819 kb on + strand, within MMJJ_RS05565 at 1040.100 kb on - strand, within MMJJ_RS05565 at 1040.456 kb on + strand, within MMJJ_RS05570
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 1,038,473 + +0.5 1,038,482 - -1.6 1,039,292 - -2.3 1,039,292 - +0.6 1,039,293 + -4.0 1,039,325 + -0.2 1,039,337 - MMJJ_RS05560 0.11 -1.7 1,039,338 + MMJJ_RS05560 0.11 +0.9 1,039,388 - MMJJ_RS05560 0.38 +0.3 1,039,388 - MMJJ_RS05560 0.38 +1.0 1,039,389 + MMJJ_RS05560 0.39 +1.2 1,039,416 + MMJJ_RS05560 0.53 +0.9 1,039,487 + -0.5 1,039,492 - +0.4 1,039,492 - -0.3 1,039,493 + -1.1 1,039,493 + +0.9 1,039,495 - +0.4 1,039,496 + +0.1 1,039,500 - +0.8 1,039,511 + -0.3 1,039,520 - +0.9 1,039,520 - +0.5 1,039,547 - -0.0 1,039,547 - +1.0 1,039,550 + +1.1 1,039,557 - -1.2 1,039,558 + +0.3 1,039,571 - -0.9 1,039,572 + -2.3 1,039,594 + +1.2 1,039,648 - -4.5 1,039,765 - MMJJ_RS05565 0.19 -2.4 1,039,767 - MMJJ_RS05565 0.19 +1.8 1,039,819 + MMJJ_RS05565 0.25 -0.2 1,040,100 - MMJJ_RS05565 0.61 -0.5 1,040,456 + MMJJ_RS05570 0.22 -2.3
Or see this region's nucleotide sequence