Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05125 and MMJJ_RS05130 are separated by 36 nucleotides MMJJ_RS05130 and MMJJ_RS05135 are separated by 18 nucleotides MMJJ_RS05135 and MMJJ_RS05140 are separated by 108 nucleotides MMJJ_RS05140 and MMJJ_RS05145 are separated by 62 nucleotides
MMJJ_RS05125: MMJJ_RS05125 - 50S ribosomal protein L31e, at 950,278 to 950,529
_RS05125
MMJJ_RS05130: MMJJ_RS05130 - translation initiation factor IF-6, at 950,566 to 951,249
_RS05130
MMJJ_RS05135: MMJJ_RS05135 - 50S ribosomal protein L18Ae, at 951,268 to 951,498
_RS05135
MMJJ_RS05140: MMJJ_RS05140 - DUF2666 domain-containing protein, at 951,607 to 952,029
_RS05140
MMJJ_RS05145: MMJJ_RS05145 - 5,10-methylenetetrahydromethanopterin reductase, at 952,092 to 953,054
_RS05145
Position (kb) 951
952 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 950.889 kb on + strand, within MMJJ_RS05130 at 950.960 kb on - strand, within MMJJ_RS05130 at 951.243 kb on + strand at 951.248 kb on + strand at 951.264 kb on - strand at 951.265 kb on + strand at 951.449 kb on - strand, within MMJJ_RS05135 at 951.496 kb on - strand at 951.496 kb on - strand at 951.505 kb on - strand at 951.514 kb on - strand at 951.515 kb on + strand at 951.540 kb on - strand at 951.545 kb on - strand at 951.546 kb on + strand at 951.547 kb on - strand at 951.547 kb on - strand at 951.550 kb on + strand at 951.554 kb on - strand at 951.563 kb on - strand at 951.565 kb on - strand at 951.565 kb on - strand at 951.565 kb on - strand at 951.565 kb on - strand at 951.577 kb on - strand at 951.579 kb on - strand at 951.579 kb on - strand at 951.580 kb on + strand at 951.583 kb on + strand at 951.606 kb on - strand at 951.623 kb on - strand at 951.653 kb on + strand, within MMJJ_RS05140 at 951.684 kb on - strand, within MMJJ_RS05140 at 951.801 kb on + strand, within MMJJ_RS05140 at 951.825 kb on - strand, within MMJJ_RS05140 at 951.825 kb on - strand, within MMJJ_RS05140 at 951.838 kb on + strand, within MMJJ_RS05140 at 951.877 kb on + strand, within MMJJ_RS05140 at 951.903 kb on - strand, within MMJJ_RS05140 at 951.954 kb on + strand, within MMJJ_RS05140 at 951.987 kb on - strand at 951.988 kb on + strand at 952.004 kb on + strand at 952.090 kb on + strand at 952.390 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 950,889 + MMJJ_RS05130 0.47 -1.5 950,960 - MMJJ_RS05130 0.58 -0.5 951,243 + -1.5 951,248 + +1.1 951,264 - +0.3 951,265 + -1.1 951,449 - MMJJ_RS05135 0.78 +0.4 951,496 - -1.2 951,496 - -0.6 951,505 - -0.4 951,514 - -3.3 951,515 + -0.7 951,540 - -2.1 951,545 - +0.3 951,546 + +0.5 951,547 - +0.4 951,547 - +0.3 951,550 + +0.6 951,554 - +0.8 951,563 - -0.7 951,565 - -0.9 951,565 - +0.6 951,565 - -0.5 951,565 - +0.1 951,577 - +2.7 951,579 - +0.2 951,579 - +1.3 951,580 + +0.6 951,583 + +1.0 951,606 - -0.3 951,623 - -0.5 951,653 + MMJJ_RS05140 0.11 -4.9 951,684 - MMJJ_RS05140 0.18 +0.3 951,801 + MMJJ_RS05140 0.46 +0.8 951,825 - MMJJ_RS05140 0.52 -0.4 951,825 - MMJJ_RS05140 0.52 +1.0 951,838 + MMJJ_RS05140 0.55 +0.1 951,877 + MMJJ_RS05140 0.64 -1.7 951,903 - MMJJ_RS05140 0.70 +0.1 951,954 + MMJJ_RS05140 0.82 +0.5 951,987 - +1.6 951,988 + +1.9 952,004 + +1.0 952,090 + -1.3 952,390 + -0.2
Or see this region's nucleotide sequence