Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS04975

Experiment: McCas-formate glass attached

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS04965 and MMJJ_RS04970 are separated by 100 nucleotidesMMJJ_RS04970 and MMJJ_RS04975 are separated by 22 nucleotidesMMJJ_RS04975 and MMJJ_RS04980 are separated by 15 nucleotidesMMJJ_RS04980 and MMJJ_RS04985 are separated by 4 nucleotides MMJJ_RS04965: MMJJ_RS04965 - RNA polymerase Rpb4 family protein, at 922,804 to 923,127 _RS04965 MMJJ_RS04970: MMJJ_RS04970 - DUF655 domain-containing protein, at 923,228 to 923,812 _RS04970 MMJJ_RS04975: MMJJ_RS04975 - glycosyltransferase family 4 protein, at 923,835 to 924,902 _RS04975 MMJJ_RS04980: MMJJ_RS04980 - bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase, at 924,918 to 925,523 _RS04980 MMJJ_RS04985: MMJJ_RS04985 - glutamyl-tRNA reductase, at 925,528 to 926,676 _RS04985 Position (kb) 923 924 925Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 922.943 kb on - strand, within MMJJ_RS04965at 922.943 kb on - strand, within MMJJ_RS04965at 922.943 kb on - strand, within MMJJ_RS04965at 922.947 kb on - strand, within MMJJ_RS04965at 922.947 kb on - strand, within MMJJ_RS04965at 922.948 kb on + strand, within MMJJ_RS04965at 922.966 kb on + strand, within MMJJ_RS04965at 923.061 kb on + strand, within MMJJ_RS04965at 923.065 kb on - strand, within MMJJ_RS04965at 923.065 kb on - strand, within MMJJ_RS04965at 923.068 kb on + strand, within MMJJ_RS04965at 923.108 kb on - strandat 923.108 kb on - strandat 923.109 kb on + strandat 923.116 kb on + strandat 923.144 kb on - strandat 923.145 kb on + strandat 923.145 kb on + strandat 923.147 kb on - strandat 923.187 kb on + strandat 923.190 kb on - strandat 923.190 kb on - strandat 923.191 kb on + strandat 923.196 kb on - strandat 923.318 kb on + strand, within MMJJ_RS04970at 923.429 kb on - strand, within MMJJ_RS04970at 923.613 kb on - strand, within MMJJ_RS04970at 923.613 kb on - strand, within MMJJ_RS04970at 923.860 kb on + strandat 924.025 kb on - strand, within MMJJ_RS04975at 924.112 kb on + strand, within MMJJ_RS04975at 924.196 kb on - strand, within MMJJ_RS04975at 924.265 kb on - strand, within MMJJ_RS04975at 924.726 kb on - strand, within MMJJ_RS04975at 924.875 kb on - strandat 924.875 kb on - strandat 924.877 kb on - strandat 924.877 kb on - strandat 924.878 kb on + strandat 924.878 kb on + strandat 924.897 kb on + strandat 924.897 kb on + strandat 924.951 kb on + strandat 925.011 kb on + strand, within MMJJ_RS04980at 925.058 kb on - strand, within MMJJ_RS04980at 925.099 kb on - strandat 925.108 kb on - strand, within MMJJ_RS04980at 925.123 kb on - strand, within MMJJ_RS04980at 925.123 kb on - strand, within MMJJ_RS04980at 925.124 kb on + strand, within MMJJ_RS04980at 925.148 kb on - strand, within MMJJ_RS04980at 925.149 kb on + strand, within MMJJ_RS04980at 925.152 kb on + strand, within MMJJ_RS04980at 925.209 kb on + strand, within MMJJ_RS04980at 925.300 kb on - strand, within MMJJ_RS04980at 925.302 kb on - strand, within MMJJ_RS04980at 925.303 kb on + strand, within MMJJ_RS04980at 925.469 kb on - strandat 925.661 kb on + strand, within MMJJ_RS04985at 925.676 kb on - strand, within MMJJ_RS04985at 925.677 kb on + strand, within MMJJ_RS04985at 925.696 kb on + strand, within MMJJ_RS04985at 925.788 kb on + strand, within MMJJ_RS04985

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas-formate glass attached
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922,943 - MMJJ_RS04965 0.43 -3.0
922,943 - MMJJ_RS04965 0.43 -3.2
922,943 - MMJJ_RS04965 0.43 -1.6
922,947 - MMJJ_RS04965 0.44 +0.6
922,947 - MMJJ_RS04965 0.44 +1.7
922,948 + MMJJ_RS04965 0.44 -2.7
922,966 + MMJJ_RS04965 0.50 -1.2
923,061 + MMJJ_RS04965 0.79 +2.8
923,065 - MMJJ_RS04965 0.81 +2.5
923,065 - MMJJ_RS04965 0.81 -0.7
923,068 + MMJJ_RS04965 0.81 -1.7
923,108 - -0.5
923,108 - +0.8
923,109 + +0.6
923,116 + -0.3
923,144 - -1.7
923,145 + -1.4
923,145 + +0.1
923,147 - -0.4
923,187 + -1.2
923,190 - -2.0
923,190 - -0.9
923,191 + -2.6
923,196 - -0.7
923,318 + MMJJ_RS04970 0.15 -2.7
923,429 - MMJJ_RS04970 0.34 -2.3
923,613 - MMJJ_RS04970 0.66 -2.8
923,613 - MMJJ_RS04970 0.66 +2.4
923,860 + -0.5
924,025 - MMJJ_RS04975 0.18 -1.3
924,112 + MMJJ_RS04975 0.26 +1.8
924,196 - MMJJ_RS04975 0.34 -2.3
924,265 - MMJJ_RS04975 0.40 -2.3
924,726 - MMJJ_RS04975 0.83 +1.9
924,875 - -0.9
924,875 - -1.7
924,877 - -1.0
924,877 - -0.4
924,878 + +2.1
924,878 + +2.7
924,897 + +0.8
924,897 + -0.1
924,951 + -1.0
925,011 + MMJJ_RS04980 0.15 -0.1
925,058 - MMJJ_RS04980 0.23 -0.3
925,099 - -1.0
925,108 - MMJJ_RS04980 0.31 -2.7
925,123 - MMJJ_RS04980 0.34 +0.7
925,123 - MMJJ_RS04980 0.34 -1.0
925,124 + MMJJ_RS04980 0.34 -1.3
925,148 - MMJJ_RS04980 0.38 -2.5
925,149 + MMJJ_RS04980 0.38 -2.7
925,152 + MMJJ_RS04980 0.39 -2.2
925,209 + MMJJ_RS04980 0.48 -2.5
925,300 - MMJJ_RS04980 0.63 -1.0
925,302 - MMJJ_RS04980 0.63 +2.6
925,303 + MMJJ_RS04980 0.64 -0.9
925,469 - +0.5
925,661 + MMJJ_RS04985 0.12 +2.5
925,676 - MMJJ_RS04985 0.13 -1.6
925,677 + MMJJ_RS04985 0.13 +0.5
925,696 + MMJJ_RS04985 0.15 -0.9
925,788 + MMJJ_RS04985 0.23 -1.3

Or see this region's nucleotide sequence