Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03485 and MMJJ_RS03490 are separated by 84 nucleotides MMJJ_RS03490 and MMJJ_RS03495 are separated by 38 nucleotides MMJJ_RS03495 and MMJJ_RS03500 are separated by 203 nucleotides
MMJJ_RS03485: MMJJ_RS03485 - radical SAM protein, at 653,657 to 655,030
_RS03485
MMJJ_RS03490: MMJJ_RS03490 - ribonuclease VapC, at 655,115 to 655,645
_RS03490
MMJJ_RS03495: MMJJ_RS03495 - hypothetical protein, at 655,684 to 655,932
_RS03495
MMJJ_RS03500: MMJJ_RS03500 - F420-dependent methylenetetrahydromethanopterin dehydrogenase, at 656,136 to 656,969
_RS03500
Position (kb) 655
656 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 654.708 kb on - strand, within MMJJ_RS03485 at 654.709 kb on + strand, within MMJJ_RS03485 at 654.709 kb on + strand, within MMJJ_RS03485 at 654.709 kb on + strand, within MMJJ_RS03485 at 654.709 kb on + strand, within MMJJ_RS03485 at 654.711 kb on - strand, within MMJJ_RS03485 at 654.711 kb on - strand, within MMJJ_RS03485 at 654.712 kb on + strand, within MMJJ_RS03485 at 654.733 kb on - strand, within MMJJ_RS03485 at 654.769 kb on - strand, within MMJJ_RS03485 at 654.769 kb on - strand, within MMJJ_RS03485 at 654.817 kb on - strand, within MMJJ_RS03485 at 654.831 kb on + strand, within MMJJ_RS03485 at 654.848 kb on - strand, within MMJJ_RS03485 at 654.869 kb on - strand, within MMJJ_RS03485 at 654.870 kb on + strand, within MMJJ_RS03485 at 654.877 kb on + strand, within MMJJ_RS03485 at 655.007 kb on + strand at 655.048 kb on + strand at 655.053 kb on - strand at 655.054 kb on + strand at 655.054 kb on + strand at 655.054 kb on + strand at 655.057 kb on - strand at 655.057 kb on - strand at 655.063 kb on - strand at 655.066 kb on - strand at 655.066 kb on - strand at 655.066 kb on - strand at 655.067 kb on + strand at 655.067 kb on + strand at 655.067 kb on + strand at 655.067 kb on + strand at 655.070 kb on - strand at 655.070 kb on - strand at 655.070 kb on - strand at 655.071 kb on + strand at 655.071 kb on + strand at 655.077 kb on + strand at 655.078 kb on - strand at 655.078 kb on - strand at 655.078 kb on - strand at 655.078 kb on - strand at 655.078 kb on - strand at 655.078 kb on - strand at 655.078 kb on - strand at 655.078 kb on - strand at 655.078 kb on - strand at 655.079 kb on + strand at 655.079 kb on + strand at 655.079 kb on + strand at 655.079 kb on + strand at 655.079 kb on + strand at 655.081 kb on - strand at 655.081 kb on - strand at 655.081 kb on - strand at 655.081 kb on - strand at 655.082 kb on + strand at 655.082 kb on + strand at 655.082 kb on + strand at 655.083 kb on - strand at 655.083 kb on - strand at 655.083 kb on - strand at 655.084 kb on + strand at 655.084 kb on + strand at 655.084 kb on + strand at 655.084 kb on + strand at 655.087 kb on - strand at 655.087 kb on - strand at 655.087 kb on - strand at 655.087 kb on - strand at 655.087 kb on - strand at 655.087 kb on - strand at 655.088 kb on + strand at 655.088 kb on + strand at 655.088 kb on + strand at 655.088 kb on + strand at 655.088 kb on + strand at 655.088 kb on + strand at 655.089 kb on - strand at 655.089 kb on - strand at 655.089 kb on - strand at 655.090 kb on + strand at 655.090 kb on + strand at 655.090 kb on + strand at 655.098 kb on + strand at 655.101 kb on - strand at 655.146 kb on - strand at 655.147 kb on + strand at 655.154 kb on - strand at 655.185 kb on + strand, within MMJJ_RS03490 at 655.353 kb on - strand, within MMJJ_RS03490 at 655.591 kb on - strand, within MMJJ_RS03490 at 655.592 kb on + strand, within MMJJ_RS03490 at 655.611 kb on + strand at 655.615 kb on - strand at 655.644 kb on + strand at 655.644 kb on + strand at 655.647 kb on + strand at 655.647 kb on - strand at 655.647 kb on - strand at 655.647 kb on - strand at 655.648 kb on + strand at 655.648 kb on + strand at 655.648 kb on + strand at 655.648 kb on + strand at 655.649 kb on - strand at 655.649 kb on - strand at 655.650 kb on + strand at 655.655 kb on + strand at 655.668 kb on - strand at 655.668 kb on - strand at 655.673 kb on - strand at 655.726 kb on - strand, within MMJJ_RS03495 at 655.744 kb on - strand, within MMJJ_RS03495 at 655.745 kb on + strand, within MMJJ_RS03495 at 655.745 kb on + strand, within MMJJ_RS03495 at 655.819 kb on - strand, within MMJJ_RS03495 at 655.820 kb on + strand, within MMJJ_RS03495 at 655.849 kb on + strand, within MMJJ_RS03495 at 655.855 kb on - strand, within MMJJ_RS03495 at 655.858 kb on + strand, within MMJJ_RS03495 at 655.885 kb on - strand, within MMJJ_RS03495 at 655.900 kb on - strand, within MMJJ_RS03495 at 655.900 kb on - strand, within MMJJ_RS03495 at 655.901 kb on + strand, within MMJJ_RS03495 at 655.970 kb on + strand at 655.977 kb on - strand at 655.977 kb on - strand at 655.978 kb on + strand at 655.978 kb on + strand at 655.979 kb on - strand at 655.980 kb on + strand at 655.985 kb on - strand at 655.996 kb on + strand at 655.999 kb on - strand at 656.000 kb on + strand at 656.023 kb on + strand at 656.035 kb on + strand at 656.116 kb on - strand at 656.133 kb on + strand at 656.134 kb on - strand at 656.135 kb on + strand at 656.173 kb on - strand at 656.188 kb on - strand at 656.245 kb on - strand, within MMJJ_RS03500 at 656.246 kb on + strand, within MMJJ_RS03500 at 656.258 kb on - strand, within MMJJ_RS03500 at 656.259 kb on + strand, within MMJJ_RS03500 at 656.278 kb on - strand, within MMJJ_RS03500 at 656.279 kb on + strand, within MMJJ_RS03500 at 656.311 kb on - strand, within MMJJ_RS03500 at 656.329 kb on - strand, within MMJJ_RS03500 at 656.330 kb on + strand, within MMJJ_RS03500 at 656.330 kb on + strand, within MMJJ_RS03500 at 656.331 kb on - strand, within MMJJ_RS03500 at 656.332 kb on + strand, within MMJJ_RS03500 at 656.332 kb on + strand, within MMJJ_RS03500 at 656.384 kb on + strand, within MMJJ_RS03500 at 656.429 kb on - strand, within MMJJ_RS03500 at 656.429 kb on - strand, within MMJJ_RS03500 at 656.518 kb on - strand, within MMJJ_RS03500 at 656.519 kb on + strand, within MMJJ_RS03500 at 656.584 kb on - strand, within MMJJ_RS03500 at 656.584 kb on - strand, within MMJJ_RS03500 at 656.585 kb on + strand, within MMJJ_RS03500 at 656.593 kb on - strand, within MMJJ_RS03500 at 656.612 kb on - strand, within MMJJ_RS03500 at 656.617 kb on - strand, within MMJJ_RS03500 at 656.618 kb on + strand, within MMJJ_RS03500 at 656.618 kb on + strand, within MMJJ_RS03500 at 656.618 kb on + strand, within MMJJ_RS03500 at 656.644 kb on - strand, within MMJJ_RS03500 at 656.726 kb on - strand, within MMJJ_RS03500 at 656.726 kb on - strand, within MMJJ_RS03500 at 656.741 kb on + strand, within MMJJ_RS03500 at 656.763 kb on + strand, within MMJJ_RS03500 at 656.806 kb on + strand, within MMJJ_RS03500 at 656.806 kb on + strand, within MMJJ_RS03500 at 656.839 kb on - strand, within MMJJ_RS03500 at 656.840 kb on + strand, within MMJJ_RS03500 at 656.840 kb on + strand, within MMJJ_RS03500 at 656.844 kb on - strand, within MMJJ_RS03500 at 656.845 kb on + strand, within MMJJ_RS03500 at 656.845 kb on + strand, within MMJJ_RS03500 at 656.857 kb on - strand, within MMJJ_RS03500
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 654,708 - MMJJ_RS03485 0.76 -1.6 654,709 + MMJJ_RS03485 0.77 +1.4 654,709 + MMJJ_RS03485 0.77 -0.2 654,709 + MMJJ_RS03485 0.77 +0.8 654,709 + MMJJ_RS03485 0.77 +0.1 654,711 - MMJJ_RS03485 0.77 -0.8 654,711 - MMJJ_RS03485 0.77 -1.7 654,712 + MMJJ_RS03485 0.77 +0.2 654,733 - MMJJ_RS03485 0.78 +0.3 654,769 - MMJJ_RS03485 0.81 -2.4 654,769 - MMJJ_RS03485 0.81 +0.3 654,817 - MMJJ_RS03485 0.84 -1.6 654,831 + MMJJ_RS03485 0.85 +0.7 654,848 - MMJJ_RS03485 0.87 +0.3 654,869 - MMJJ_RS03485 0.88 -2.6 654,870 + MMJJ_RS03485 0.88 -1.1 654,877 + MMJJ_RS03485 0.89 +0.4 655,007 + -0.0 655,048 + +1.0 655,053 - +0.6 655,054 + +0.4 655,054 + +0.7 655,054 + +0.3 655,057 - -1.6 655,057 - +1.0 655,063 - -0.4 655,066 - -4.4 655,066 - +0.3 655,066 - -1.8 655,067 + -2.0 655,067 + -0.6 655,067 + -0.6 655,067 + +0.5 655,070 - -0.4 655,070 - +0.0 655,070 - +0.1 655,071 + -0.0 655,071 + +1.0 655,077 + +0.6 655,078 - -0.0 655,078 - -0.0 655,078 - +0.9 655,078 - +0.7 655,078 - +0.2 655,078 - +0.1 655,078 - -2.9 655,078 - -0.1 655,078 - -2.1 655,079 + -1.9 655,079 + -0.6 655,079 + +0.0 655,079 + +0.9 655,079 + -0.9 655,081 - +0.9 655,081 - +0.2 655,081 - -6.0 655,081 - +0.7 655,082 + -0.6 655,082 + +0.3 655,082 + +0.4 655,083 - +0.0 655,083 - -0.3 655,083 - -0.8 655,084 + -5.7 655,084 + +0.2 655,084 + +0.5 655,084 + +0.1 655,087 - -0.1 655,087 - +0.1 655,087 - -1.2 655,087 - -0.8 655,087 - +0.2 655,087 - -1.2 655,088 + -0.1 655,088 + -0.1 655,088 + +0.4 655,088 + -2.5 655,088 + +0.4 655,088 + -1.0 655,089 - +0.0 655,089 - -0.2 655,089 - +0.0 655,090 + -1.6 655,090 + -0.6 655,090 + +0.4 655,098 + -2.1 655,101 - +0.6 655,146 - -1.5 655,147 + -1.4 655,154 - +2.4 655,185 + MMJJ_RS03490 0.13 +1.9 655,353 - MMJJ_RS03490 0.45 -0.5 655,591 - MMJJ_RS03490 0.90 -1.9 655,592 + MMJJ_RS03490 0.90 +0.4 655,611 + +0.1 655,615 - -2.9 655,644 + +0.6 655,644 + +0.3 655,647 + +0.2 655,647 - +0.1 655,647 - -1.1 655,647 - +0.6 655,648 + +0.3 655,648 + -1.0 655,648 + -0.4 655,648 + +0.4 655,649 - -0.3 655,649 - -1.1 655,650 + +2.4 655,655 + -3.2 655,668 - +0.6 655,668 - -0.4 655,673 - -0.9 655,726 - MMJJ_RS03495 0.17 +0.4 655,744 - MMJJ_RS03495 0.24 +0.0 655,745 + MMJJ_RS03495 0.24 +0.4 655,745 + MMJJ_RS03495 0.24 -1.8 655,819 - MMJJ_RS03495 0.54 +0.5 655,820 + MMJJ_RS03495 0.55 -0.0 655,849 + MMJJ_RS03495 0.66 +0.4 655,855 - MMJJ_RS03495 0.69 -0.2 655,858 + MMJJ_RS03495 0.70 +0.6 655,885 - MMJJ_RS03495 0.81 +0.7 655,900 - MMJJ_RS03495 0.87 +0.7 655,900 - MMJJ_RS03495 0.87 +0.7 655,901 + MMJJ_RS03495 0.87 +0.2 655,970 + -0.3 655,977 - +0.2 655,977 - +1.6 655,978 + -1.4 655,978 + -0.1 655,979 - -0.4 655,980 + -0.8 655,985 - -4.6 655,996 + -1.6 655,999 - -0.6 656,000 + +0.1 656,023 + +0.3 656,035 + -0.0 656,116 - -0.4 656,133 + -2.5 656,134 - -1.0 656,135 + +0.2 656,173 - +0.0 656,188 - -0.5 656,245 - MMJJ_RS03500 0.13 +1.6 656,246 + MMJJ_RS03500 0.13 -2.0 656,258 - MMJJ_RS03500 0.15 +0.0 656,259 + MMJJ_RS03500 0.15 +0.3 656,278 - MMJJ_RS03500 0.17 +0.0 656,279 + MMJJ_RS03500 0.17 -0.1 656,311 - MMJJ_RS03500 0.21 -0.8 656,329 - MMJJ_RS03500 0.23 -0.2 656,330 + MMJJ_RS03500 0.23 +0.5 656,330 + MMJJ_RS03500 0.23 +0.7 656,331 - MMJJ_RS03500 0.23 -2.4 656,332 + MMJJ_RS03500 0.24 +0.1 656,332 + MMJJ_RS03500 0.24 +0.7 656,384 + MMJJ_RS03500 0.30 +0.3 656,429 - MMJJ_RS03500 0.35 -2.1 656,429 - MMJJ_RS03500 0.35 -0.7 656,518 - MMJJ_RS03500 0.46 +0.5 656,519 + MMJJ_RS03500 0.46 -1.3 656,584 - MMJJ_RS03500 0.54 +0.6 656,584 - MMJJ_RS03500 0.54 +0.4 656,585 + MMJJ_RS03500 0.54 +0.9 656,593 - MMJJ_RS03500 0.55 -0.2 656,612 - MMJJ_RS03500 0.57 +1.0 656,617 - MMJJ_RS03500 0.58 +0.4 656,618 + MMJJ_RS03500 0.58 -1.8 656,618 + MMJJ_RS03500 0.58 +1.5 656,618 + MMJJ_RS03500 0.58 +0.1 656,644 - MMJJ_RS03500 0.61 +0.1 656,726 - MMJJ_RS03500 0.71 -1.1 656,726 - MMJJ_RS03500 0.71 -1.0 656,741 + MMJJ_RS03500 0.73 -0.2 656,763 + MMJJ_RS03500 0.75 +0.4 656,806 + MMJJ_RS03500 0.80 -1.2 656,806 + MMJJ_RS03500 0.80 -1.4 656,839 - MMJJ_RS03500 0.84 -0.8 656,840 + MMJJ_RS03500 0.84 -3.0 656,840 + MMJJ_RS03500 0.84 +0.5 656,844 - MMJJ_RS03500 0.85 +0.1 656,845 + MMJJ_RS03500 0.85 -3.7 656,845 + MMJJ_RS03500 0.85 -0.7 656,857 - MMJJ_RS03500 0.86 -0.1
Or see this region's nucleotide sequence