Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03175 and MMJJ_RS03180 are separated by 10 nucleotides MMJJ_RS03180 and MMJJ_RS03185 are separated by 42 nucleotides MMJJ_RS03185 and MMJJ_RS03190 are separated by 199 nucleotides
MMJJ_RS03175: MMJJ_RS03175 - zinc metalloprotease HtpX, at 599,977 to 600,834
_RS03175
MMJJ_RS03180: MMJJ_RS03180 - pantoate kinase, at 600,845 to 601,720
_RS03180
MMJJ_RS03185: MMJJ_RS03185 - alanine--tRNA ligase, at 601,763 to 604,441
_RS03185
MMJJ_RS03190: MMJJ_RS03190 - phosphopyruvate hydratase, at 604,641 to 605,933
_RS03190
Position (kb) 601
602
603
604
605 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 600.794 kb on + strand at 600.794 kb on + strand at 600.808 kb on - strand at 600.808 kb on - strand at 600.809 kb on + strand at 600.821 kb on + strand at 600.825 kb on - strand at 600.825 kb on - strand at 600.825 kb on - strand at 600.839 kb on + strand at 600.839 kb on + strand at 600.846 kb on - strand at 601.490 kb on + strand, within MMJJ_RS03180 at 601.886 kb on - strand at 602.199 kb on + strand, within MMJJ_RS03185 at 603.581 kb on + strand, within MMJJ_RS03185 at 604.330 kb on - strand at 604.331 kb on + strand at 604.375 kb on - strand at 604.497 kb on + strand at 604.541 kb on - strand at 604.542 kb on + strand at 604.542 kb on + strand at 604.546 kb on - strand at 604.547 kb on + strand at 604.548 kb on - strand at 604.562 kb on + strand at 604.586 kb on - strand at 604.595 kb on - strand at 604.600 kb on + strand at 604.626 kb on - strand at 604.632 kb on - strand at 604.632 kb on - strand at 604.633 kb on + strand at 604.643 kb on + strand at 604.643 kb on + strand at 605.171 kb on + strand, within MMJJ_RS03190 at 605.304 kb on - strand at 605.310 kb on - strand at 605.311 kb on + strand, within MMJJ_RS03190 at 605.340 kb on - strand, within MMJJ_RS03190 at 605.360 kb on + strand, within MMJJ_RS03190
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 600,794 + +0.9 600,794 + -2.1 600,808 - +0.1 600,808 - -2.2 600,809 + -0.6 600,821 + -0.6 600,825 - -1.3 600,825 - +1.8 600,825 - +0.1 600,839 + +0.3 600,839 + +0.2 600,846 - -2.9 601,490 + MMJJ_RS03180 0.74 -1.0 601,886 - +0.2 602,199 + MMJJ_RS03185 0.16 +2.5 603,581 + MMJJ_RS03185 0.68 +0.8 604,330 - -0.1 604,331 + -1.1 604,375 - -1.6 604,497 + -0.4 604,541 - +0.7 604,542 + +0.6 604,542 + +0.1 604,546 - -0.6 604,547 + +1.1 604,548 - +0.6 604,562 + -1.0 604,586 - -0.7 604,595 - +0.8 604,600 + -0.1 604,626 - -0.2 604,632 - +0.1 604,632 - +0.0 604,633 + -0.2 604,643 + +0.9 604,643 + +0.3 605,171 + MMJJ_RS03190 0.41 -0.4 605,304 - +0.6 605,310 - -0.1 605,311 + MMJJ_RS03190 0.52 -0.2 605,340 - MMJJ_RS03190 0.54 -0.4 605,360 + MMJJ_RS03190 0.56 +2.7
Or see this region's nucleotide sequence