Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02945 and MMJJ_RS02950 are separated by 93 nucleotides MMJJ_RS02950 and MMJJ_RS02955 are separated by 3 nucleotides MMJJ_RS02955 and MMJJ_RS02960 overlap by 14 nucleotides MMJJ_RS02960 and MMJJ_RS02965 are separated by 0 nucleotides
MMJJ_RS02945: MMJJ_RS02945 - dihydroorotate dehydrogenase, at 549,447 to 550,361
_RS02945
MMJJ_RS02950: MMJJ_RS02950 - epoxyqueuosine reductase, at 550,455 to 551,246
_RS02950
MMJJ_RS02955: MMJJ_RS02955 - glutamate-cysteine ligase family protein, at 551,250 to 552,353
_RS02955
MMJJ_RS02960: MMJJ_RS02960 - RimK family alpha-L-glutamate ligase, at 552,340 to 553,290
_RS02960
MMJJ_RS02965: MMJJ_RS02965 - RimK-like ATPgrasp N-terminal domain-containing protein, at 553,291 to 553,896
_RS02965
Position (kb) 551
552
553 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 550.398 kb on - strand at 550.405 kb on - strand at 550.407 kb on - strand at 550.407 kb on - strand at 550.427 kb on - strand at 550.428 kb on + strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.431 kb on - strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.432 kb on + strand at 550.440 kb on + strand at 550.455 kb on + strand at 550.465 kb on - strand at 550.526 kb on - strand at 550.546 kb on - strand, within MMJJ_RS02950 at 550.579 kb on - strand, within MMJJ_RS02950 at 550.585 kb on - strand, within MMJJ_RS02950 at 550.651 kb on - strand, within MMJJ_RS02950 at 550.651 kb on - strand, within MMJJ_RS02950 at 550.682 kb on - strand, within MMJJ_RS02950 at 550.683 kb on + strand, within MMJJ_RS02950 at 550.696 kb on - strand, within MMJJ_RS02950 at 550.696 kb on - strand, within MMJJ_RS02950 at 550.751 kb on - strand, within MMJJ_RS02950 at 550.751 kb on - strand, within MMJJ_RS02950 at 550.752 kb on + strand, within MMJJ_RS02950 at 550.752 kb on + strand, within MMJJ_RS02950 at 550.752 kb on + strand, within MMJJ_RS02950 at 550.805 kb on - strand, within MMJJ_RS02950 at 550.805 kb on - strand, within MMJJ_RS02950 at 550.805 kb on - strand, within MMJJ_RS02950 at 550.806 kb on + strand, within MMJJ_RS02950 at 550.806 kb on + strand, within MMJJ_RS02950 at 550.860 kb on - strand, within MMJJ_RS02950 at 550.860 kb on - strand, within MMJJ_RS02950 at 550.897 kb on - strand, within MMJJ_RS02950 at 550.897 kb on - strand, within MMJJ_RS02950 at 550.898 kb on + strand, within MMJJ_RS02950 at 550.898 kb on + strand, within MMJJ_RS02950 at 550.901 kb on - strand, within MMJJ_RS02950 at 550.902 kb on + strand, within MMJJ_RS02950 at 550.919 kb on - strand, within MMJJ_RS02950 at 550.919 kb on - strand, within MMJJ_RS02950 at 550.919 kb on - strand, within MMJJ_RS02950 at 550.920 kb on + strand, within MMJJ_RS02950 at 550.920 kb on + strand, within MMJJ_RS02950 at 550.920 kb on + strand, within MMJJ_RS02950 at 550.924 kb on - strand, within MMJJ_RS02950 at 550.924 kb on - strand, within MMJJ_RS02950 at 550.929 kb on + strand, within MMJJ_RS02950 at 550.944 kb on + strand, within MMJJ_RS02950 at 550.964 kb on + strand, within MMJJ_RS02950 at 550.967 kb on - strand, within MMJJ_RS02950 at 550.967 kb on - strand, within MMJJ_RS02950 at 550.968 kb on + strand, within MMJJ_RS02950 at 550.968 kb on + strand, within MMJJ_RS02950 at 550.988 kb on - strand, within MMJJ_RS02950 at 551.021 kb on - strand, within MMJJ_RS02950 at 551.021 kb on - strand, within MMJJ_RS02950 at 551.021 kb on - strand, within MMJJ_RS02950 at 551.022 kb on + strand, within MMJJ_RS02950 at 551.022 kb on + strand, within MMJJ_RS02950 at 551.025 kb on - strand, within MMJJ_RS02950 at 551.106 kb on - strand, within MMJJ_RS02950 at 551.113 kb on - strand, within MMJJ_RS02950 at 551.223 kb on - strand at 551.225 kb on - strand at 551.225 kb on - strand at 551.298 kb on - strand at 551.298 kb on - strand at 551.309 kb on - strand at 551.309 kb on - strand at 551.333 kb on - strand at 551.339 kb on - strand at 551.339 kb on - strand at 551.339 kb on - strand at 551.340 kb on + strand at 551.385 kb on - strand, within MMJJ_RS02955 at 551.386 kb on + strand, within MMJJ_RS02955 at 551.386 kb on + strand, within MMJJ_RS02955 at 551.386 kb on + strand, within MMJJ_RS02955 at 551.390 kb on + strand, within MMJJ_RS02955 at 551.405 kb on - strand, within MMJJ_RS02955 at 551.405 kb on - strand, within MMJJ_RS02955 at 551.521 kb on + strand, within MMJJ_RS02955 at 551.644 kb on + strand, within MMJJ_RS02955 at 551.806 kb on - strand, within MMJJ_RS02955 at 551.806 kb on - strand, within MMJJ_RS02955 at 551.807 kb on + strand, within MMJJ_RS02955 at 551.807 kb on + strand, within MMJJ_RS02955 at 551.809 kb on - strand, within MMJJ_RS02955 at 551.810 kb on + strand, within MMJJ_RS02955 at 551.810 kb on + strand, within MMJJ_RS02955 at 551.829 kb on + strand, within MMJJ_RS02955 at 551.829 kb on + strand, within MMJJ_RS02955 at 551.829 kb on + strand, within MMJJ_RS02955 at 551.829 kb on + strand, within MMJJ_RS02955 at 551.830 kb on - strand, within MMJJ_RS02955 at 551.830 kb on - strand, within MMJJ_RS02955 at 551.830 kb on - strand, within MMJJ_RS02955 at 551.830 kb on - strand, within MMJJ_RS02955 at 551.830 kb on - strand, within MMJJ_RS02955 at 551.831 kb on + strand, within MMJJ_RS02955 at 551.871 kb on - strand, within MMJJ_RS02955 at 551.877 kb on - strand, within MMJJ_RS02955 at 551.878 kb on + strand, within MMJJ_RS02955 at 551.885 kb on + strand, within MMJJ_RS02955 at 551.889 kb on - strand, within MMJJ_RS02955 at 551.946 kb on - strand, within MMJJ_RS02955 at 551.946 kb on - strand, within MMJJ_RS02955 at 551.947 kb on + strand, within MMJJ_RS02955 at 551.949 kb on + strand, within MMJJ_RS02955 at 551.953 kb on + strand, within MMJJ_RS02955 at 551.953 kb on + strand, within MMJJ_RS02955 at 551.958 kb on - strand, within MMJJ_RS02955 at 552.000 kb on - strand, within MMJJ_RS02955 at 552.010 kb on - strand, within MMJJ_RS02955 at 552.010 kb on - strand, within MMJJ_RS02955 at 552.011 kb on + strand, within MMJJ_RS02955 at 552.011 kb on + strand, within MMJJ_RS02955 at 552.011 kb on + strand, within MMJJ_RS02955 at 552.011 kb on + strand, within MMJJ_RS02955 at 552.066 kb on - strand, within MMJJ_RS02955 at 552.066 kb on - strand, within MMJJ_RS02955 at 552.072 kb on + strand, within MMJJ_RS02955 at 552.100 kb on - strand, within MMJJ_RS02955 at 552.104 kb on - strand, within MMJJ_RS02955 at 552.105 kb on + strand, within MMJJ_RS02955 at 552.105 kb on + strand, within MMJJ_RS02955 at 552.105 kb on + strand, within MMJJ_RS02955 at 552.107 kb on - strand, within MMJJ_RS02955 at 552.132 kb on - strand, within MMJJ_RS02955 at 552.132 kb on - strand, within MMJJ_RS02955 at 552.140 kb on - strand, within MMJJ_RS02955 at 552.167 kb on - strand, within MMJJ_RS02955 at 552.182 kb on - strand, within MMJJ_RS02955 at 552.182 kb on - strand, within MMJJ_RS02955 at 552.198 kb on - strand, within MMJJ_RS02955 at 552.201 kb on - strand, within MMJJ_RS02955 at 552.213 kb on - strand, within MMJJ_RS02955 at 552.376 kb on - strand at 552.376 kb on - strand at 552.432 kb on - strand at 552.433 kb on + strand at 552.434 kb on - strand at 552.434 kb on - strand at 552.434 kb on - strand at 552.434 kb on - strand at 552.434 kb on - strand at 552.434 kb on - strand at 552.435 kb on + strand, within MMJJ_RS02960 at 552.435 kb on + strand, within MMJJ_RS02960 at 552.435 kb on + strand, within MMJJ_RS02960 at 552.435 kb on + strand, within MMJJ_RS02960 at 552.435 kb on + strand, within MMJJ_RS02960 at 552.435 kb on + strand, within MMJJ_RS02960 at 552.435 kb on + strand, within MMJJ_RS02960 at 552.447 kb on - strand, within MMJJ_RS02960 at 552.506 kb on + strand, within MMJJ_RS02960 at 552.514 kb on - strand, within MMJJ_RS02960 at 552.515 kb on + strand, within MMJJ_RS02960 at 552.515 kb on + strand, within MMJJ_RS02960 at 552.515 kb on + strand, within MMJJ_RS02960 at 552.518 kb on - strand, within MMJJ_RS02960 at 552.518 kb on - strand, within MMJJ_RS02960 at 552.518 kb on - strand, within MMJJ_RS02960 at 552.598 kb on - strand, within MMJJ_RS02960 at 552.601 kb on + strand, within MMJJ_RS02960 at 552.629 kb on + strand, within MMJJ_RS02960 at 552.642 kb on - strand, within MMJJ_RS02960 at 552.643 kb on + strand, within MMJJ_RS02960 at 552.648 kb on + strand, within MMJJ_RS02960 at 552.648 kb on + strand, within MMJJ_RS02960 at 552.650 kb on + strand, within MMJJ_RS02960 at 552.676 kb on - strand, within MMJJ_RS02960 at 552.676 kb on - strand, within MMJJ_RS02960 at 552.705 kb on + strand, within MMJJ_RS02960 at 552.705 kb on + strand, within MMJJ_RS02960 at 552.717 kb on + strand, within MMJJ_RS02960 at 552.734 kb on + strand, within MMJJ_RS02960 at 552.787 kb on - strand, within MMJJ_RS02960 at 552.787 kb on - strand, within MMJJ_RS02960 at 552.787 kb on - strand, within MMJJ_RS02960 at 552.817 kb on - strand, within MMJJ_RS02960 at 552.835 kb on - strand, within MMJJ_RS02960 at 552.893 kb on + strand, within MMJJ_RS02960 at 552.940 kb on + strand, within MMJJ_RS02960 at 552.940 kb on + strand, within MMJJ_RS02960 at 552.940 kb on + strand, within MMJJ_RS02960 at 552.979 kb on - strand, within MMJJ_RS02960 at 552.979 kb on - strand, within MMJJ_RS02960 at 552.979 kb on - strand, within MMJJ_RS02960 at 552.984 kb on - strand, within MMJJ_RS02960 at 552.992 kb on + strand, within MMJJ_RS02960 at 553.005 kb on + strand, within MMJJ_RS02960 at 553.013 kb on + strand, within MMJJ_RS02960 at 553.051 kb on - strand, within MMJJ_RS02960 at 553.099 kb on - strand, within MMJJ_RS02960 at 553.126 kb on - strand, within MMJJ_RS02960 at 553.151 kb on + strand, within MMJJ_RS02960 at 553.160 kb on + strand, within MMJJ_RS02960 at 553.209 kb on - strand at 553.248 kb on - strand at 553.248 kb on - strand at 553.248 kb on - strand at 553.249 kb on + strand at 553.249 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 550,398 - -0.5 550,405 - -0.6 550,407 - -0.6 550,407 - -2.1 550,427 - +0.5 550,428 + +2.0 550,431 - +0.8 550,431 - -1.7 550,431 - -1.0 550,431 - -0.4 550,431 - +0.3 550,431 - +0.6 550,431 - +0.4 550,431 - +0.2 550,431 - -0.2 550,432 + +0.2 550,432 + -0.5 550,432 + +0.1 550,432 + +1.0 550,432 + +0.7 550,432 + +1.4 550,432 + +0.5 550,432 + -1.3 550,432 + -0.1 550,432 + +0.6 550,440 + +0.2 550,455 + -1.0 550,465 - -2.0 550,526 - -0.4 550,546 - MMJJ_RS02950 0.11 +0.1 550,579 - MMJJ_RS02950 0.16 +0.4 550,585 - MMJJ_RS02950 0.16 +1.1 550,651 - MMJJ_RS02950 0.25 +1.0 550,651 - MMJJ_RS02950 0.25 +1.1 550,682 - MMJJ_RS02950 0.29 -0.0 550,683 + MMJJ_RS02950 0.29 +0.3 550,696 - MMJJ_RS02950 0.30 +0.1 550,696 - MMJJ_RS02950 0.30 -0.8 550,751 - MMJJ_RS02950 0.37 -0.7 550,751 - MMJJ_RS02950 0.37 -0.3 550,752 + MMJJ_RS02950 0.38 -0.3 550,752 + MMJJ_RS02950 0.38 +0.8 550,752 + MMJJ_RS02950 0.38 +0.0 550,805 - MMJJ_RS02950 0.44 -1.1 550,805 - MMJJ_RS02950 0.44 -0.3 550,805 - MMJJ_RS02950 0.44 +0.0 550,806 + MMJJ_RS02950 0.44 +0.8 550,806 + MMJJ_RS02950 0.44 -1.8 550,860 - MMJJ_RS02950 0.51 +0.5 550,860 - MMJJ_RS02950 0.51 +0.2 550,897 - MMJJ_RS02950 0.56 -0.1 550,897 - MMJJ_RS02950 0.56 -0.9 550,898 + MMJJ_RS02950 0.56 -0.1 550,898 + MMJJ_RS02950 0.56 +0.1 550,901 - MMJJ_RS02950 0.56 +0.1 550,902 + MMJJ_RS02950 0.56 +1.1 550,919 - MMJJ_RS02950 0.59 +0.1 550,919 - MMJJ_RS02950 0.59 +0.3 550,919 - MMJJ_RS02950 0.59 -0.7 550,920 + MMJJ_RS02950 0.59 +0.4 550,920 + MMJJ_RS02950 0.59 -0.0 550,920 + MMJJ_RS02950 0.59 -2.6 550,924 - MMJJ_RS02950 0.59 -0.2 550,924 - MMJJ_RS02950 0.59 -4.1 550,929 + MMJJ_RS02950 0.60 -4.8 550,944 + MMJJ_RS02950 0.62 -0.3 550,964 + MMJJ_RS02950 0.64 +0.5 550,967 - MMJJ_RS02950 0.65 -2.5 550,967 - MMJJ_RS02950 0.65 +0.3 550,968 + MMJJ_RS02950 0.65 +0.7 550,968 + MMJJ_RS02950 0.65 -2.4 550,988 - MMJJ_RS02950 0.67 -0.4 551,021 - MMJJ_RS02950 0.71 -0.7 551,021 - MMJJ_RS02950 0.71 +1.1 551,021 - MMJJ_RS02950 0.71 +0.9 551,022 + MMJJ_RS02950 0.72 +0.3 551,022 + MMJJ_RS02950 0.72 +0.2 551,025 - MMJJ_RS02950 0.72 -0.2 551,106 - MMJJ_RS02950 0.82 +2.8 551,113 - MMJJ_RS02950 0.83 -0.1 551,223 - +0.1 551,225 - +0.8 551,225 - +0.9 551,298 - -1.2 551,298 - +1.3 551,309 - +0.9 551,309 - +0.7 551,333 - +0.3 551,339 - +1.3 551,339 - -0.2 551,339 - -0.1 551,340 + +0.4 551,385 - MMJJ_RS02955 0.12 -0.1 551,386 + MMJJ_RS02955 0.12 +0.5 551,386 + MMJJ_RS02955 0.12 +0.7 551,386 + MMJJ_RS02955 0.12 +0.8 551,390 + MMJJ_RS02955 0.13 -0.1 551,405 - MMJJ_RS02955 0.14 +0.6 551,405 - MMJJ_RS02955 0.14 +0.9 551,521 + MMJJ_RS02955 0.25 +1.0 551,644 + MMJJ_RS02955 0.36 -1.0 551,806 - MMJJ_RS02955 0.50 -0.4 551,806 - MMJJ_RS02955 0.50 -2.6 551,807 + MMJJ_RS02955 0.50 -0.2 551,807 + MMJJ_RS02955 0.50 -1.5 551,809 - MMJJ_RS02955 0.51 +0.6 551,810 + MMJJ_RS02955 0.51 -1.5 551,810 + MMJJ_RS02955 0.51 +0.1 551,829 + MMJJ_RS02955 0.52 +3.4 551,829 + MMJJ_RS02955 0.52 +0.0 551,829 + MMJJ_RS02955 0.52 +0.5 551,829 + MMJJ_RS02955 0.52 -3.3 551,830 - MMJJ_RS02955 0.53 -0.4 551,830 - MMJJ_RS02955 0.53 +0.1 551,830 - MMJJ_RS02955 0.53 +0.0 551,830 - MMJJ_RS02955 0.53 -0.2 551,830 - MMJJ_RS02955 0.53 +0.5 551,831 + MMJJ_RS02955 0.53 +1.1 551,871 - MMJJ_RS02955 0.56 -0.1 551,877 - MMJJ_RS02955 0.57 +0.5 551,878 + MMJJ_RS02955 0.57 +0.9 551,885 + MMJJ_RS02955 0.58 +0.5 551,889 - MMJJ_RS02955 0.58 +0.6 551,946 - MMJJ_RS02955 0.63 -0.0 551,946 - MMJJ_RS02955 0.63 +0.3 551,947 + MMJJ_RS02955 0.63 +0.5 551,949 + MMJJ_RS02955 0.63 +0.9 551,953 + MMJJ_RS02955 0.64 +1.1 551,953 + MMJJ_RS02955 0.64 +1.0 551,958 - MMJJ_RS02955 0.64 -0.5 552,000 - MMJJ_RS02955 0.68 +0.8 552,010 - MMJJ_RS02955 0.69 +0.6 552,010 - MMJJ_RS02955 0.69 +0.1 552,011 + MMJJ_RS02955 0.69 -0.5 552,011 + MMJJ_RS02955 0.69 -0.7 552,011 + MMJJ_RS02955 0.69 -0.1 552,011 + MMJJ_RS02955 0.69 -2.0 552,066 - MMJJ_RS02955 0.74 +1.6 552,066 - MMJJ_RS02955 0.74 +0.1 552,072 + MMJJ_RS02955 0.74 +0.5 552,100 - MMJJ_RS02955 0.77 -0.3 552,104 - MMJJ_RS02955 0.77 +0.1 552,105 + MMJJ_RS02955 0.77 -0.7 552,105 + MMJJ_RS02955 0.77 -0.8 552,105 + MMJJ_RS02955 0.77 +0.3 552,107 - MMJJ_RS02955 0.78 +0.5 552,132 - MMJJ_RS02955 0.80 +1.2 552,132 - MMJJ_RS02955 0.80 +0.1 552,140 - MMJJ_RS02955 0.81 +0.9 552,167 - MMJJ_RS02955 0.83 -0.7 552,182 - MMJJ_RS02955 0.84 +0.9 552,182 - MMJJ_RS02955 0.84 -0.1 552,198 - MMJJ_RS02955 0.86 -0.5 552,201 - MMJJ_RS02955 0.86 -1.9 552,213 - MMJJ_RS02955 0.87 -0.5 552,376 - -0.1 552,376 - +0.3 552,432 - +0.5 552,433 + +0.9 552,434 - -1.5 552,434 - -3.1 552,434 - -0.3 552,434 - -1.1 552,434 - +1.0 552,434 - -0.3 552,435 + MMJJ_RS02960 0.10 -1.2 552,435 + MMJJ_RS02960 0.10 +0.3 552,435 + MMJJ_RS02960 0.10 -2.8 552,435 + MMJJ_RS02960 0.10 +0.3 552,435 + MMJJ_RS02960 0.10 +0.0 552,435 + MMJJ_RS02960 0.10 +0.8 552,435 + MMJJ_RS02960 0.10 +2.4 552,447 - MMJJ_RS02960 0.11 +0.1 552,506 + MMJJ_RS02960 0.17 +1.2 552,514 - MMJJ_RS02960 0.18 -0.3 552,515 + MMJJ_RS02960 0.18 +0.7 552,515 + MMJJ_RS02960 0.18 -0.9 552,515 + MMJJ_RS02960 0.18 -1.4 552,518 - MMJJ_RS02960 0.19 -1.0 552,518 - MMJJ_RS02960 0.19 -0.5 552,518 - MMJJ_RS02960 0.19 +0.1 552,598 - MMJJ_RS02960 0.27 +0.3 552,601 + MMJJ_RS02960 0.27 -1.5 552,629 + MMJJ_RS02960 0.30 +0.2 552,642 - MMJJ_RS02960 0.32 +1.0 552,643 + MMJJ_RS02960 0.32 -0.8 552,648 + MMJJ_RS02960 0.32 -1.2 552,648 + MMJJ_RS02960 0.32 -0.4 552,650 + MMJJ_RS02960 0.33 -0.2 552,676 - MMJJ_RS02960 0.35 +0.1 552,676 - MMJJ_RS02960 0.35 +0.1 552,705 + MMJJ_RS02960 0.38 +0.2 552,705 + MMJJ_RS02960 0.38 -1.3 552,717 + MMJJ_RS02960 0.40 +0.6 552,734 + MMJJ_RS02960 0.41 +0.3 552,787 - MMJJ_RS02960 0.47 +0.0 552,787 - MMJJ_RS02960 0.47 -0.0 552,787 - MMJJ_RS02960 0.47 +0.5 552,817 - MMJJ_RS02960 0.50 +0.9 552,835 - MMJJ_RS02960 0.52 +0.2 552,893 + MMJJ_RS02960 0.58 +0.0 552,940 + MMJJ_RS02960 0.63 +0.2 552,940 + MMJJ_RS02960 0.63 +0.4 552,940 + MMJJ_RS02960 0.63 +0.0 552,979 - MMJJ_RS02960 0.67 -0.1 552,979 - MMJJ_RS02960 0.67 -0.3 552,979 - MMJJ_RS02960 0.67 +0.5 552,984 - MMJJ_RS02960 0.68 +0.8 552,992 + MMJJ_RS02960 0.69 -0.2 553,005 + MMJJ_RS02960 0.70 +0.2 553,013 + MMJJ_RS02960 0.71 +0.2 553,051 - MMJJ_RS02960 0.75 -0.6 553,099 - MMJJ_RS02960 0.80 -1.2 553,126 - MMJJ_RS02960 0.83 -0.5 553,151 + MMJJ_RS02960 0.85 -3.0 553,160 + MMJJ_RS02960 0.86 +1.2 553,209 - -0.3 553,248 - +0.8 553,248 - -3.9 553,248 - -0.6 553,249 + -2.6 553,249 + +0.5
Or see this region's nucleotide sequence