Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02860 and MMJJ_RS02865 are separated by 103 nucleotides MMJJ_RS02865 and MMJJ_RS02870 are separated by 115 nucleotides MMJJ_RS02870 and MMJJ_RS02875 are separated by 127 nucleotides
MMJJ_RS02860: MMJJ_RS02860 - site-specific integrase, at 539,832 to 540,380
_RS02860
MMJJ_RS02865: MMJJ_RS02865 - amino acid ABC transporter substrate-binding protein, at 540,484 to 541,230
_RS02865
MMJJ_RS02870: MMJJ_RS02870 - hypothetical protein, at 541,346 to 541,867
_RS02870
MMJJ_RS02875: MMJJ_RS02875 - site-2 protease family protein, at 541,995 to 543,008
_RS02875
Position (kb) 540
541
542 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 539.725 kb on - strand at 539.725 kb on - strand at 539.726 kb on + strand at 539.726 kb on + strand at 539.727 kb on - strand at 539.727 kb on - strand at 539.820 kb on - strand at 539.821 kb on + strand at 539.845 kb on + strand at 539.876 kb on + strand at 539.897 kb on + strand, within MMJJ_RS02860 at 540.224 kb on - strand, within MMJJ_RS02860 at 540.295 kb on - strand, within MMJJ_RS02860 at 540.412 kb on - strand at 540.426 kb on + strand at 540.450 kb on + strand at 540.452 kb on - strand at 540.455 kb on + strand at 540.464 kb on - strand at 540.477 kb on - strand at 540.478 kb on + strand at 540.524 kb on - strand at 540.540 kb on - strand at 540.540 kb on - strand at 540.540 kb on - strand at 540.541 kb on + strand at 540.567 kb on - strand, within MMJJ_RS02865 at 540.712 kb on - strand, within MMJJ_RS02865 at 540.713 kb on + strand, within MMJJ_RS02865 at 540.814 kb on + strand, within MMJJ_RS02865 at 540.814 kb on + strand, within MMJJ_RS02865 at 540.923 kb on - strand, within MMJJ_RS02865 at 540.971 kb on - strand, within MMJJ_RS02865 at 540.972 kb on + strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.000 kb on - strand, within MMJJ_RS02865 at 541.001 kb on + strand, within MMJJ_RS02865 at 541.069 kb on - strand, within MMJJ_RS02865 at 541.089 kb on + strand, within MMJJ_RS02865 at 541.089 kb on + strand, within MMJJ_RS02865 at 541.096 kb on + strand, within MMJJ_RS02865 at 541.096 kb on + strand, within MMJJ_RS02865 at 541.128 kb on + strand, within MMJJ_RS02865 at 541.149 kb on + strand, within MMJJ_RS02865 at 541.196 kb on + strand at 541.228 kb on - strand at 541.260 kb on - strand at 541.261 kb on + strand at 541.264 kb on + strand at 541.264 kb on + strand at 541.264 kb on + strand at 541.278 kb on + strand at 541.278 kb on + strand at 541.358 kb on - strand at 541.367 kb on - strand at 541.368 kb on + strand at 541.368 kb on + strand at 541.372 kb on - strand at 541.373 kb on + strand at 541.436 kb on + strand, within MMJJ_RS02870 at 541.454 kb on - strand, within MMJJ_RS02870 at 541.455 kb on + strand, within MMJJ_RS02870 at 541.518 kb on - strand, within MMJJ_RS02870 at 541.518 kb on - strand, within MMJJ_RS02870 at 541.565 kb on + strand, within MMJJ_RS02870 at 541.566 kb on - strand, within MMJJ_RS02870 at 541.567 kb on + strand, within MMJJ_RS02870 at 541.629 kb on + strand, within MMJJ_RS02870 at 541.720 kb on - strand, within MMJJ_RS02870 at 541.748 kb on - strand, within MMJJ_RS02870 at 541.751 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.762 kb on + strand, within MMJJ_RS02870 at 541.793 kb on + strand, within MMJJ_RS02870 at 541.827 kb on - strand at 541.833 kb on - strand at 541.842 kb on - strand at 541.842 kb on - strand at 541.848 kb on + strand at 541.877 kb on - strand at 541.882 kb on - strand at 541.882 kb on - strand at 541.883 kb on + strand at 541.904 kb on + strand at 541.918 kb on - strand at 541.919 kb on + strand at 541.919 kb on + strand at 541.922 kb on - strand at 541.922 kb on - strand at 541.922 kb on - strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.923 kb on + strand at 541.926 kb on - strand at 541.926 kb on - strand at 541.927 kb on + strand at 541.927 kb on + strand at 541.927 kb on + strand at 541.943 kb on + strand at 541.946 kb on + strand at 541.948 kb on - strand at 541.948 kb on - strand at 541.949 kb on + strand at 541.952 kb on - strand at 541.953 kb on + strand at 541.965 kb on - strand at 541.965 kb on - strand at 541.965 kb on - strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.966 kb on + strand at 541.967 kb on - strand at 541.967 kb on - strand at 541.967 kb on - strand at 541.968 kb on + strand at 541.968 kb on + strand at 541.970 kb on - strand at 541.970 kb on - strand at 541.970 kb on - strand at 541.970 kb on - strand at 541.971 kb on + strand at 541.971 kb on + strand at 541.971 kb on + strand at 541.971 kb on + strand at 541.971 kb on + strand at 542.045 kb on - strand at 542.048 kb on - strand at 542.132 kb on + strand, within MMJJ_RS02875 at 542.157 kb on - strand, within MMJJ_RS02875 at 542.157 kb on - strand, within MMJJ_RS02875 at 542.158 kb on + strand, within MMJJ_RS02875 at 542.158 kb on + strand, within MMJJ_RS02875 at 542.179 kb on - strand, within MMJJ_RS02875
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 539,725 - +0.5 539,725 - -1.8 539,726 + -0.1 539,726 + +0.0 539,727 - +1.1 539,727 - -0.8 539,820 - +0.4 539,821 + +1.1 539,845 + +0.5 539,876 + -0.4 539,897 + MMJJ_RS02860 0.12 +0.1 540,224 - MMJJ_RS02860 0.71 -0.2 540,295 - MMJJ_RS02860 0.84 -1.2 540,412 - -0.7 540,426 + -0.0 540,450 + -0.4 540,452 - -1.0 540,455 + -2.2 540,464 - -1.6 540,477 - +0.3 540,478 + +0.2 540,524 - -0.0 540,540 - +1.6 540,540 - -1.5 540,540 - +1.2 540,541 + +0.8 540,567 - MMJJ_RS02865 0.11 +0.6 540,712 - MMJJ_RS02865 0.31 +0.7 540,713 + MMJJ_RS02865 0.31 +1.1 540,814 + MMJJ_RS02865 0.44 +0.5 540,814 + MMJJ_RS02865 0.44 +0.6 540,923 - MMJJ_RS02865 0.59 +1.0 540,971 - MMJJ_RS02865 0.65 -0.8 540,972 + MMJJ_RS02865 0.65 -0.5 541,000 - MMJJ_RS02865 0.69 +0.5 541,000 - MMJJ_RS02865 0.69 +0.1 541,000 - MMJJ_RS02865 0.69 +0.5 541,000 - MMJJ_RS02865 0.69 -0.1 541,001 + MMJJ_RS02865 0.69 +0.2 541,069 - MMJJ_RS02865 0.78 +0.7 541,089 + MMJJ_RS02865 0.81 +0.5 541,089 + MMJJ_RS02865 0.81 -0.5 541,096 + MMJJ_RS02865 0.82 +1.5 541,096 + MMJJ_RS02865 0.82 -1.2 541,128 + MMJJ_RS02865 0.86 -0.7 541,149 + MMJJ_RS02865 0.89 +0.8 541,196 + +0.1 541,228 - +0.7 541,260 - +0.2 541,261 + +1.8 541,264 + -1.5 541,264 + +0.6 541,264 + +0.4 541,278 + +0.4 541,278 + +0.0 541,358 - -0.4 541,367 - +1.6 541,368 + -0.8 541,368 + +0.0 541,372 - -1.0 541,373 + -2.1 541,436 + MMJJ_RS02870 0.17 +0.7 541,454 - MMJJ_RS02870 0.21 -0.1 541,455 + MMJJ_RS02870 0.21 -0.7 541,518 - MMJJ_RS02870 0.33 +0.2 541,518 - MMJJ_RS02870 0.33 +1.0 541,565 + MMJJ_RS02870 0.42 -0.1 541,566 - MMJJ_RS02870 0.42 -0.7 541,567 + MMJJ_RS02870 0.42 -0.8 541,629 + MMJJ_RS02870 0.54 -0.2 541,720 - MMJJ_RS02870 0.72 +1.0 541,748 - MMJJ_RS02870 0.77 +1.3 541,751 + MMJJ_RS02870 0.78 -0.5 541,762 + MMJJ_RS02870 0.80 +0.7 541,762 + MMJJ_RS02870 0.80 +0.2 541,762 + MMJJ_RS02870 0.80 +0.2 541,762 + MMJJ_RS02870 0.80 -0.2 541,793 + MMJJ_RS02870 0.86 -0.7 541,827 - -0.5 541,833 - +1.3 541,842 - +0.2 541,842 - -0.1 541,848 + +0.6 541,877 - +1.0 541,882 - +0.8 541,882 - +0.7 541,883 + -0.5 541,904 + -0.1 541,918 - +0.6 541,919 + -2.8 541,919 + +1.2 541,922 - +0.6 541,922 - -1.0 541,922 - +0.4 541,923 + +0.2 541,923 + +0.6 541,923 + +0.8 541,923 + +0.2 541,923 + -3.2 541,923 + -1.0 541,923 + -1.0 541,926 - +0.8 541,926 - -0.1 541,927 + -4.4 541,927 + +0.4 541,927 + -0.4 541,943 + -2.4 541,946 + +0.6 541,948 - +0.2 541,948 - -0.3 541,949 + -0.9 541,952 - -0.4 541,953 + -0.3 541,965 - -1.4 541,965 - -0.4 541,965 - -0.3 541,966 + +0.3 541,966 + +0.4 541,966 + +0.2 541,966 + +0.3 541,966 + +0.2 541,966 + +0.2 541,967 - -2.1 541,967 - -0.5 541,967 - -3.5 541,968 + -0.9 541,968 + -0.7 541,970 - +1.2 541,970 - +0.4 541,970 - +1.0 541,970 - +0.6 541,971 + +0.2 541,971 + +0.9 541,971 + +0.8 541,971 + -0.6 541,971 + +0.1 542,045 - -0.4 542,048 - -0.4 542,132 + MMJJ_RS02875 0.14 -0.9 542,157 - MMJJ_RS02875 0.16 +0.5 542,157 - MMJJ_RS02875 0.16 -0.1 542,158 + MMJJ_RS02875 0.16 +1.0 542,158 + MMJJ_RS02875 0.16 -0.0 542,179 - MMJJ_RS02875 0.18 -0.4
Or see this region's nucleotide sequence