Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01820

Experiment: McCas-formate glass attached

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01810 and MMJJ_RS01815 are separated by 22 nucleotidesMMJJ_RS01815 and MMJJ_RS01820 are separated by 10 nucleotidesMMJJ_RS01820 and MMJJ_RS01825 are separated by 105 nucleotidesMMJJ_RS01825 and MMJJ_RS01830 are separated by 46 nucleotides MMJJ_RS01810: MMJJ_RS01810 - methyl coenzyme M reductase system, component A2, at 347,308 to 348,903 _RS01810 MMJJ_RS01815: MMJJ_RS01815 - DUF134 domain-containing protein, at 348,926 to 349,321 _RS01815 MMJJ_RS01820: MMJJ_RS01820 - MJ1244 family protein, at 349,332 to 349,661 _RS01820 MMJJ_RS01825: MMJJ_RS01825 - DNA repair and recombination protein RadB, at 349,767 to 350,417 _RS01825 MMJJ_RS01830: MMJJ_RS01830 - tRNA(Ile)(2)-agmatinylcytidine synthase, at 350,464 to 351,738 _RS01830 Position (kb) 349 350Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 348.850 kb on - strandat 349.022 kb on - strand, within MMJJ_RS01815at 349.027 kb on - strand, within MMJJ_RS01815at 349.046 kb on + strand, within MMJJ_RS01815at 349.065 kb on + strand, within MMJJ_RS01815at 349.211 kb on - strand, within MMJJ_RS01815at 349.252 kb on - strand, within MMJJ_RS01815at 349.256 kb on - strand, within MMJJ_RS01815at 349.333 kb on - strandat 349.333 kb on - strandat 349.382 kb on - strand, within MMJJ_RS01820at 349.382 kb on - strand, within MMJJ_RS01820at 349.382 kb on - strand, within MMJJ_RS01820at 349.382 kb on - strand, within MMJJ_RS01820at 349.383 kb on + strand, within MMJJ_RS01820at 349.383 kb on + strand, within MMJJ_RS01820at 349.383 kb on + strand, within MMJJ_RS01820at 349.383 kb on + strand, within MMJJ_RS01820at 349.480 kb on + strand, within MMJJ_RS01820at 349.677 kb on - strandat 349.677 kb on - strandat 349.677 kb on - strandat 349.696 kb on - strandat 349.696 kb on - strandat 349.696 kb on - strandat 349.712 kb on - strandat 349.714 kb on - strandat 349.714 kb on - strandat 349.714 kb on - strandat 349.714 kb on - strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.716 kb on - strandat 349.718 kb on - strandat 349.719 kb on + strandat 349.751 kb on + strandat 349.781 kb on - strandat 349.809 kb on - strandat 349.863 kb on + strand, within MMJJ_RS01825at 349.863 kb on + strand, within MMJJ_RS01825at 349.921 kb on + strand, within MMJJ_RS01825at 349.954 kb on + strand, within MMJJ_RS01825at 349.954 kb on + strand, within MMJJ_RS01825at 349.976 kb on - strand, within MMJJ_RS01825at 350.055 kb on + strand, within MMJJ_RS01825at 350.093 kb on + strand, within MMJJ_RS01825at 350.120 kb on - strand, within MMJJ_RS01825at 350.120 kb on - strand, within MMJJ_RS01825at 350.121 kb on + strand, within MMJJ_RS01825at 350.121 kb on + strand, within MMJJ_RS01825at 350.211 kb on - strand, within MMJJ_RS01825at 350.334 kb on + strand, within MMJJ_RS01825at 350.373 kb on - strandat 350.417 kb on - strandat 350.418 kb on + strandat 350.444 kb on + strandat 350.449 kb on - strandat 350.461 kb on + strandat 350.461 kb on + strandat 350.465 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas-formate glass attached
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348,850 - -0.9
349,022 - MMJJ_RS01815 0.24 +0.3
349,027 - MMJJ_RS01815 0.26 -0.8
349,046 + MMJJ_RS01815 0.30 +0.3
349,065 + MMJJ_RS01815 0.35 +0.6
349,211 - MMJJ_RS01815 0.72 -0.7
349,252 - MMJJ_RS01815 0.82 +0.7
349,256 - MMJJ_RS01815 0.83 +0.9
349,333 - +1.5
349,333 - -4.5
349,382 - MMJJ_RS01820 0.15 +0.8
349,382 - MMJJ_RS01820 0.15 -1.7
349,382 - MMJJ_RS01820 0.15 +1.4
349,382 - MMJJ_RS01820 0.15 +0.3
349,383 + MMJJ_RS01820 0.15 +1.4
349,383 + MMJJ_RS01820 0.15 +0.8
349,383 + MMJJ_RS01820 0.15 +0.7
349,383 + MMJJ_RS01820 0.15 -4.2
349,480 + MMJJ_RS01820 0.45 +0.1
349,677 - -1.2
349,677 - +0.3
349,677 - +2.3
349,696 - -1.9
349,696 - +1.4
349,696 - +2.0
349,712 - -0.5
349,714 - -0.3
349,714 - +0.5
349,714 - -1.2
349,714 - -1.3
349,715 + +1.8
349,715 + -2.6
349,715 + +1.0
349,715 + -0.3
349,715 + -0.1
349,716 - +0.6
349,718 - +0.9
349,719 + -0.1
349,751 + +0.9
349,781 - +0.5
349,809 - +1.0
349,863 + MMJJ_RS01825 0.15 -2.4
349,863 + MMJJ_RS01825 0.15 +0.8
349,921 + MMJJ_RS01825 0.24 +0.4
349,954 + MMJJ_RS01825 0.29 +0.3
349,954 + MMJJ_RS01825 0.29 +0.3
349,976 - MMJJ_RS01825 0.32 -2.3
350,055 + MMJJ_RS01825 0.44 -1.9
350,093 + MMJJ_RS01825 0.50 -1.3
350,120 - MMJJ_RS01825 0.54 -1.1
350,120 - MMJJ_RS01825 0.54 -1.1
350,121 + MMJJ_RS01825 0.54 -0.8
350,121 + MMJJ_RS01825 0.54 -4.0
350,211 - MMJJ_RS01825 0.68 -0.0
350,334 + MMJJ_RS01825 0.87 -1.1
350,373 - -1.0
350,417 - +0.5
350,418 + -1.4
350,444 + -0.4
350,449 - +0.9
350,461 + -2.2
350,461 + +1.6
350,465 - -0.2

Or see this region's nucleotide sequence