Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01515

Experiment: McCas-formate glass attached

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01510 and MMJJ_RS01515 are separated by 130 nucleotidesMMJJ_RS01515 and MMJJ_RS01520 are separated by 10 nucleotidesMMJJ_RS01520 and MMJJ_RS01525 are separated by 14 nucleotides MMJJ_RS01510: MMJJ_RS01510 - ATP-binding protein, at 295,702 to 297,099 _RS01510 MMJJ_RS01515: MMJJ_RS01515 - DUF2178 domain-containing protein, at 297,230 to 297,616 _RS01515 MMJJ_RS01520: MMJJ_RS01520 - helix-turn-helix transcriptional regulator, at 297,627 to 297,830 _RS01520 MMJJ_RS01525: MMJJ_RS01525 - ketopantoate reductase family protein, at 297,845 to 298,765 _RS01525 Position (kb) 297 298Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 296.248 kb on + strand, within MMJJ_RS01510at 296.265 kb on - strandat 296.265 kb on - strand, within MMJJ_RS01510at 296.304 kb on - strand, within MMJJ_RS01510at 296.337 kb on - strand, within MMJJ_RS01510at 296.378 kb on + strand, within MMJJ_RS01510at 296.384 kb on + strand, within MMJJ_RS01510at 296.409 kb on - strand, within MMJJ_RS01510at 296.443 kb on + strand, within MMJJ_RS01510at 296.451 kb on + strand, within MMJJ_RS01510at 296.463 kb on - strand, within MMJJ_RS01510at 296.501 kb on - strand, within MMJJ_RS01510at 296.501 kb on - strand, within MMJJ_RS01510at 296.586 kb on + strand, within MMJJ_RS01510at 296.653 kb on - strand, within MMJJ_RS01510at 296.712 kb on + strand, within MMJJ_RS01510at 296.744 kb on - strand, within MMJJ_RS01510at 296.874 kb on - strand, within MMJJ_RS01510at 296.916 kb on - strand, within MMJJ_RS01510at 297.015 kb on + strandat 297.066 kb on - strandat 297.069 kb on + strandat 297.073 kb on - strandat 297.074 kb on + strandat 297.076 kb on + strandat 297.088 kb on - strandat 297.107 kb on + strandat 297.115 kb on - strandat 297.120 kb on - strandat 297.121 kb on + strandat 297.126 kb on + strandat 297.126 kb on + strandat 297.126 kb on + strandat 297.129 kb on + strandat 297.195 kb on + strandat 297.208 kb on - strandat 297.254 kb on - strandat 297.259 kb on - strandat 297.273 kb on - strand, within MMJJ_RS01515at 297.274 kb on + strand, within MMJJ_RS01515at 297.321 kb on + strand, within MMJJ_RS01515at 297.343 kb on + strand, within MMJJ_RS01515at 297.370 kb on - strand, within MMJJ_RS01515at 297.371 kb on + strand, within MMJJ_RS01515at 297.394 kb on + strand, within MMJJ_RS01515at 297.394 kb on + strand, within MMJJ_RS01515at 297.504 kb on + strand, within MMJJ_RS01515at 297.504 kb on + strand, within MMJJ_RS01515at 297.552 kb on - strand, within MMJJ_RS01515at 297.567 kb on - strand, within MMJJ_RS01515at 297.567 kb on - strand, within MMJJ_RS01515at 297.588 kb on - strandat 297.588 kb on - strandat 297.588 kb on - strandat 297.588 kb on - strandat 297.588 kb on - strandat 297.589 kb on + strandat 297.589 kb on + strandat 297.589 kb on + strandat 297.589 kb on + strandat 297.589 kb on + strandat 297.589 kb on + strandat 297.589 kb on + strandat 297.641 kb on - strandat 297.651 kb on + strand, within MMJJ_RS01520at 297.717 kb on + strand, within MMJJ_RS01520at 297.757 kb on - strand, within MMJJ_RS01520at 297.757 kb on - strand, within MMJJ_RS01520at 297.779 kb on + strand, within MMJJ_RS01520at 297.802 kb on - strand, within MMJJ_RS01520at 297.802 kb on - strand, within MMJJ_RS01520at 297.807 kb on + strand, within MMJJ_RS01520at 297.842 kb on - strandat 297.864 kb on + strandat 297.901 kb on - strandat 297.906 kb on - strandat 297.965 kb on - strand, within MMJJ_RS01525at 297.965 kb on - strand, within MMJJ_RS01525at 297.976 kb on - strand, within MMJJ_RS01525at 297.976 kb on - strand, within MMJJ_RS01525at 297.977 kb on + strand, within MMJJ_RS01525at 298.059 kb on - strand, within MMJJ_RS01525at 298.124 kb on - strand, within MMJJ_RS01525at 298.129 kb on - strand, within MMJJ_RS01525at 298.130 kb on + strand, within MMJJ_RS01525at 298.130 kb on + strand, within MMJJ_RS01525at 298.135 kb on - strand, within MMJJ_RS01525at 298.141 kb on - strand, within MMJJ_RS01525at 298.142 kb on + strand, within MMJJ_RS01525at 298.142 kb on + strand, within MMJJ_RS01525at 298.186 kb on - strand, within MMJJ_RS01525at 298.217 kb on + strand, within MMJJ_RS01525at 298.218 kb on - strand, within MMJJ_RS01525at 298.250 kb on + strand, within MMJJ_RS01525at 298.361 kb on - strand, within MMJJ_RS01525at 298.362 kb on + strand, within MMJJ_RS01525at 298.408 kb on - strand, within MMJJ_RS01525at 298.412 kb on + strand, within MMJJ_RS01525at 298.412 kb on + strand, within MMJJ_RS01525at 298.412 kb on + strand, within MMJJ_RS01525at 298.430 kb on + strand, within MMJJ_RS01525at 298.435 kb on - strand, within MMJJ_RS01525at 298.435 kb on - strand, within MMJJ_RS01525at 298.436 kb on + strand, within MMJJ_RS01525at 298.436 kb on + strand, within MMJJ_RS01525at 298.437 kb on - strand, within MMJJ_RS01525at 298.443 kb on + strand, within MMJJ_RS01525at 298.500 kb on + strand, within MMJJ_RS01525at 298.500 kb on + strand, within MMJJ_RS01525at 298.501 kb on - strandat 298.501 kb on - strand, within MMJJ_RS01525at 298.553 kb on - strand, within MMJJ_RS01525at 298.554 kb on + strand, within MMJJ_RS01525at 298.555 kb on - strand, within MMJJ_RS01525at 298.555 kb on - strand, within MMJJ_RS01525at 298.556 kb on + strand, within MMJJ_RS01525at 298.558 kb on - strand, within MMJJ_RS01525at 298.558 kb on - strand, within MMJJ_RS01525at 298.559 kb on + strand, within MMJJ_RS01525at 298.561 kb on - strand, within MMJJ_RS01525at 298.563 kb on - strand, within MMJJ_RS01525at 298.563 kb on - strand, within MMJJ_RS01525

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas-formate glass attached
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296,248 + MMJJ_RS01510 0.39 +0.5
296,265 - +0.2
296,265 - MMJJ_RS01510 0.40 +0.2
296,304 - MMJJ_RS01510 0.43 -2.4
296,337 - MMJJ_RS01510 0.45 -4.1
296,378 + MMJJ_RS01510 0.48 +1.4
296,384 + MMJJ_RS01510 0.49 +0.2
296,409 - MMJJ_RS01510 0.51 +0.7
296,443 + MMJJ_RS01510 0.53 +0.6
296,451 + MMJJ_RS01510 0.54 -0.6
296,463 - MMJJ_RS01510 0.54 +0.0
296,501 - MMJJ_RS01510 0.57 +0.2
296,501 - MMJJ_RS01510 0.57 -2.3
296,586 + MMJJ_RS01510 0.63 -0.7
296,653 - MMJJ_RS01510 0.68 -3.1
296,712 + MMJJ_RS01510 0.72 -0.9
296,744 - MMJJ_RS01510 0.75 -1.8
296,874 - MMJJ_RS01510 0.84 +0.1
296,916 - MMJJ_RS01510 0.87 +0.6
297,015 + -0.6
297,066 - -0.2
297,069 + +0.4
297,073 - -2.8
297,074 + +0.3
297,076 + -0.1
297,088 - -2.6
297,107 + +1.3
297,115 - +0.7
297,120 - +0.5
297,121 + -0.8
297,126 + -0.1
297,126 + -2.5
297,126 + -0.3
297,129 + -0.1
297,195 + +0.1
297,208 - +0.5
297,254 - -1.1
297,259 - +0.1
297,273 - MMJJ_RS01515 0.11 +0.4
297,274 + MMJJ_RS01515 0.11 -2.0
297,321 + MMJJ_RS01515 0.24 +0.7
297,343 + MMJJ_RS01515 0.29 +0.8
297,370 - MMJJ_RS01515 0.36 +0.7
297,371 + MMJJ_RS01515 0.36 +1.1
297,394 + MMJJ_RS01515 0.42 +1.5
297,394 + MMJJ_RS01515 0.42 -0.5
297,504 + MMJJ_RS01515 0.71 +0.3
297,504 + MMJJ_RS01515 0.71 -0.1
297,552 - MMJJ_RS01515 0.83 +0.6
297,567 - MMJJ_RS01515 0.87 +0.7
297,567 - MMJJ_RS01515 0.87 +0.1
297,588 - -0.9
297,588 - +0.1
297,588 - -0.1
297,588 - +1.3
297,588 - -0.3
297,589 + +0.0
297,589 + +1.0
297,589 + +0.3
297,589 + -0.1
297,589 + -0.1
297,589 + +0.8
297,589 + -2.4
297,641 - -0.4
297,651 + MMJJ_RS01520 0.12 -1.5
297,717 + MMJJ_RS01520 0.44 +1.3
297,757 - MMJJ_RS01520 0.64 -1.7
297,757 - MMJJ_RS01520 0.64 +0.7
297,779 + MMJJ_RS01520 0.75 +0.1
297,802 - MMJJ_RS01520 0.86 -1.4
297,802 - MMJJ_RS01520 0.86 +0.3
297,807 + MMJJ_RS01520 0.88 +0.6
297,842 - -3.1
297,864 + +0.4
297,901 - +0.1
297,906 - -0.0
297,965 - MMJJ_RS01525 0.13 +0.7
297,965 - MMJJ_RS01525 0.13 +0.5
297,976 - MMJJ_RS01525 0.14 +0.2
297,976 - MMJJ_RS01525 0.14 +0.8
297,977 + MMJJ_RS01525 0.14 +0.3
298,059 - MMJJ_RS01525 0.23 -0.0
298,124 - MMJJ_RS01525 0.30 +0.4
298,129 - MMJJ_RS01525 0.31 +0.1
298,130 + MMJJ_RS01525 0.31 -0.8
298,130 + MMJJ_RS01525 0.31 -0.7
298,135 - MMJJ_RS01525 0.31 +1.1
298,141 - MMJJ_RS01525 0.32 -0.0
298,142 + MMJJ_RS01525 0.32 -2.9
298,142 + MMJJ_RS01525 0.32 -0.2
298,186 - MMJJ_RS01525 0.37 -0.2
298,217 + MMJJ_RS01525 0.40 +0.3
298,218 - MMJJ_RS01525 0.40 +0.1
298,250 + MMJJ_RS01525 0.44 +0.3
298,361 - MMJJ_RS01525 0.56 -0.2
298,362 + MMJJ_RS01525 0.56 -5.5
298,408 - MMJJ_RS01525 0.61 -1.1
298,412 + MMJJ_RS01525 0.62 -0.7
298,412 + MMJJ_RS01525 0.62 -0.7
298,412 + MMJJ_RS01525 0.62 +0.7
298,430 + MMJJ_RS01525 0.64 -1.5
298,435 - MMJJ_RS01525 0.64 -1.2
298,435 - MMJJ_RS01525 0.64 +0.5
298,436 + MMJJ_RS01525 0.64 -0.7
298,436 + MMJJ_RS01525 0.64 +1.5
298,437 - MMJJ_RS01525 0.64 +0.3
298,443 + MMJJ_RS01525 0.65 +0.5
298,500 + MMJJ_RS01525 0.71 +0.5
298,500 + MMJJ_RS01525 0.71 -2.2
298,501 - +2.5
298,501 - MMJJ_RS01525 0.71 -4.6
298,553 - MMJJ_RS01525 0.77 +0.4
298,554 + MMJJ_RS01525 0.77 -1.2
298,555 - MMJJ_RS01525 0.77 -1.0
298,555 - MMJJ_RS01525 0.77 +0.8
298,556 + MMJJ_RS01525 0.77 +0.3
298,558 - MMJJ_RS01525 0.77 -0.6
298,558 - MMJJ_RS01525 0.77 +0.2
298,559 + MMJJ_RS01525 0.78 -0.1
298,561 - MMJJ_RS01525 0.78 +0.2
298,563 - MMJJ_RS01525 0.78 +0.5
298,563 - MMJJ_RS01525 0.78 +0.6

Or see this region's nucleotide sequence