Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01135 and MMJJ_RS01140 are separated by 308 nucleotides MMJJ_RS01140 and MMJJ_RS01150 are separated by 331 nucleotides MMJJ_RS01150 and MMJJ_RS01155 are separated by 35 nucleotides MMJJ_RS01155 and MMJJ_RS01160 are separated by 271 nucleotides
MMJJ_RS01135: MMJJ_RS01135 - hypothetical protein, at 218,626 to 222,324
_RS01135
MMJJ_RS01140: MMJJ_RS01140 - helix-turn-helix domain-containing protein, at 222,633 to 222,836
_RS01140
MMJJ_RS01150: MMJJ_RS01150 - hypothetical protein, at 223,168 to 223,587
_RS01150
MMJJ_RS01155: MMJJ_RS01155 - hypothetical protein, at 223,623 to 224,075
_RS01155
MMJJ_RS01160: MMJJ_RS01160 - AAA family ATPase, at 224,347 to 225,390
_RS01160
Position (kb) 223
224 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 222.175 kb on + strand at 222.178 kb on + strand at 222.250 kb on + strand at 222.276 kb on - strand at 222.277 kb on + strand at 222.326 kb on - strand at 222.329 kb on - strand at 222.546 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.589 kb on + strand at 222.589 kb on + strand at 222.589 kb on + strand at 222.589 kb on + strand at 222.590 kb on - strand at 222.590 kb on - strand at 222.595 kb on - strand at 222.595 kb on - strand at 222.596 kb on + strand at 222.607 kb on - strand at 222.607 kb on - strand at 222.608 kb on + strand at 222.608 kb on + strand at 222.608 kb on + strand at 222.608 kb on + strand at 222.608 kb on + strand at 222.609 kb on - strand at 222.609 kb on - strand at 222.617 kb on - strand at 222.617 kb on - strand at 222.617 kb on - strand at 222.618 kb on + strand at 222.628 kb on - strand at 222.629 kb on + strand at 222.661 kb on - strand, within MMJJ_RS01140 at 222.662 kb on + strand, within MMJJ_RS01140 at 222.675 kb on - strand, within MMJJ_RS01140 at 222.675 kb on - strand, within MMJJ_RS01140 at 222.705 kb on - strand, within MMJJ_RS01140 at 222.708 kb on + strand, within MMJJ_RS01140 at 222.725 kb on - strand, within MMJJ_RS01140 at 222.737 kb on + strand, within MMJJ_RS01140 at 222.815 kb on + strand, within MMJJ_RS01140 at 222.815 kb on + strand, within MMJJ_RS01140 at 222.894 kb on - strand at 222.910 kb on - strand at 222.910 kb on - strand at 223.030 kb on - strand at 223.031 kb on + strand at 223.098 kb on + strand at 223.137 kb on - strand at 223.144 kb on - strand at 223.145 kb on + strand at 223.179 kb on - strand at 223.179 kb on - strand at 223.184 kb on + strand at 223.184 kb on + strand at 223.258 kb on - strand, within MMJJ_RS01150 at 223.259 kb on + strand, within MMJJ_RS01150 at 223.259 kb on + strand, within MMJJ_RS01150 at 223.270 kb on - strand, within MMJJ_RS01150 at 223.346 kb on - strand, within MMJJ_RS01150 at 223.492 kb on + strand, within MMJJ_RS01150 at 223.620 kb on - strand at 223.620 kb on - strand at 223.647 kb on + strand at 223.649 kb on - strand at 223.649 kb on - strand at 223.649 kb on - strand at 223.650 kb on + strand at 223.651 kb on - strand at 223.651 kb on - strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.656 kb on + strand at 223.656 kb on + strand at 223.750 kb on - strand, within MMJJ_RS01155 at 223.782 kb on - strand, within MMJJ_RS01155 at 223.929 kb on + strand, within MMJJ_RS01155 at 223.944 kb on + strand, within MMJJ_RS01155 at 223.950 kb on - strand, within MMJJ_RS01155 at 223.952 kb on - strand, within MMJJ_RS01155 at 224.035 kb on - strand at 224.060 kb on + strand at 224.081 kb on - strand at 224.222 kb on - strand at 224.223 kb on + strand at 224.265 kb on + strand at 224.267 kb on - strand at 224.268 kb on + strand at 224.269 kb on - strand at 224.271 kb on - strand at 224.272 kb on + strand at 224.272 kb on + strand at 224.272 kb on + strand at 224.278 kb on - strand at 224.279 kb on + strand at 224.279 kb on + strand at 224.306 kb on + strand at 224.355 kb on + strand at 224.361 kb on + strand at 224.380 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 222,175 + +0.1 222,178 + -0.7 222,250 + +0.9 222,276 - -0.3 222,277 + -0.6 222,326 - +0.3 222,329 - -0.8 222,546 - +0.0 222,588 - +0.1 222,588 - +0.5 222,588 - -0.1 222,588 - -0.0 222,588 - -0.3 222,588 - +0.6 222,588 - -0.1 222,589 + +0.2 222,589 + -0.1 222,589 + +0.3 222,589 + +0.1 222,590 - -1.7 222,590 - -1.2 222,595 - -1.1 222,595 - -1.1 222,596 + +0.6 222,607 - +1.0 222,607 - +0.0 222,608 + -0.3 222,608 + +0.7 222,608 + +0.8 222,608 + +0.4 222,608 + +0.3 222,609 - +0.2 222,609 - -4.3 222,617 - +1.2 222,617 - +1.3 222,617 - -0.8 222,618 + -0.8 222,628 - +0.6 222,629 + +0.3 222,661 - MMJJ_RS01140 0.14 -0.0 222,662 + MMJJ_RS01140 0.14 +0.7 222,675 - MMJJ_RS01140 0.21 -0.1 222,675 - MMJJ_RS01140 0.21 +1.2 222,705 - MMJJ_RS01140 0.35 -0.4 222,708 + MMJJ_RS01140 0.37 +1.0 222,725 - MMJJ_RS01140 0.45 +0.2 222,737 + MMJJ_RS01140 0.51 +0.2 222,815 + MMJJ_RS01140 0.89 +0.1 222,815 + MMJJ_RS01140 0.89 -2.8 222,894 - -1.1 222,910 - +2.6 222,910 - +0.8 223,030 - +0.1 223,031 + +0.1 223,098 + +0.9 223,137 - -2.3 223,144 - -1.4 223,145 + +0.6 223,179 - -0.2 223,179 - +0.1 223,184 + -0.4 223,184 + +0.2 223,258 - MMJJ_RS01150 0.21 -0.4 223,259 + MMJJ_RS01150 0.22 +0.0 223,259 + MMJJ_RS01150 0.22 -0.4 223,270 - MMJJ_RS01150 0.24 +0.0 223,346 - MMJJ_RS01150 0.42 -0.1 223,492 + MMJJ_RS01150 0.77 +2.4 223,620 - +1.6 223,620 - +1.3 223,647 + +0.5 223,649 - +0.7 223,649 - +0.4 223,649 - -0.3 223,650 + +0.4 223,651 - +0.7 223,651 - -1.4 223,652 + +0.1 223,652 + +1.2 223,652 + -0.0 223,652 + +0.3 223,652 + +0.9 223,652 + +0.4 223,652 + -1.6 223,652 + -0.6 223,652 + +0.1 223,652 + +0.6 223,652 + -2.0 223,656 + -0.9 223,656 + -0.1 223,750 - MMJJ_RS01155 0.28 -0.3 223,782 - MMJJ_RS01155 0.35 -0.1 223,929 + MMJJ_RS01155 0.68 +0.5 223,944 + MMJJ_RS01155 0.71 -0.8 223,950 - MMJJ_RS01155 0.72 -0.1 223,952 - MMJJ_RS01155 0.73 -1.1 224,035 - -0.3 224,060 + -0.1 224,081 - +0.2 224,222 - +0.0 224,223 + -0.4 224,265 + +1.1 224,267 - +1.8 224,268 + -1.4 224,269 - +0.2 224,271 - -3.3 224,272 + +0.9 224,272 + -1.0 224,272 + +0.4 224,278 - +1.6 224,279 + +0.9 224,279 + -0.0 224,306 + +0.4 224,355 + -2.0 224,361 + +0.9 224,380 - -3.0
Or see this region's nucleotide sequence