Experiment: McCas-formate glass attached
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00495 and MMJJ_RS00500 are separated by 163 nucleotides MMJJ_RS00500 and MMJJ_RS00505 are separated by 10 nucleotides MMJJ_RS00505 and MMJJ_RS00510 are separated by 231 nucleotides
MMJJ_RS00495: MMJJ_RS00495 - CTP synthase (glutamine hydrolyzing), at 91,678 to 93,279
_RS00495
MMJJ_RS00500: MMJJ_RS00500 - GNAT family N-acetyltransferase, at 93,443 to 93,991
_RS00500
MMJJ_RS00505: MMJJ_RS00505 - 3'-5' exonuclease, at 94,002 to 94,577
_RS00505
MMJJ_RS00510: MMJJ_RS00510 - DEAD/DEAH box helicase, at 94,809 to 97,049
_RS00510
Position (kb)
93
94 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 93.270 kb on - strand at 93.381 kb on - strand at 93.381 kb on - strand at 93.382 kb on + strand at 93.382 kb on + strand at 93.382 kb on + strand at 93.382 kb on + strand at 93.386 kb on + strand at 93.398 kb on - strand at 93.398 kb on - strand at 93.399 kb on + strand at 93.399 kb on + strand at 93.399 kb on + strand at 93.406 kb on + strand at 93.411 kb on - strand at 93.412 kb on + strand at 93.413 kb on - strand at 93.413 kb on - strand at 93.413 kb on - strand at 93.413 kb on - strand at 93.414 kb on + strand at 93.443 kb on + strand at 93.455 kb on + strand at 93.455 kb on + strand at 93.459 kb on - strand at 93.460 kb on + strand at 93.460 kb on + strand at 93.486 kb on - strand at 93.486 kb on - strand at 93.486 kb on - strand at 93.486 kb on - strand at 93.601 kb on - strand, within MMJJ_RS00500 at 93.632 kb on + strand, within MMJJ_RS00500 at 93.653 kb on + strand, within MMJJ_RS00500 at 93.656 kb on + strand, within MMJJ_RS00500 at 93.691 kb on - strand, within MMJJ_RS00500 at 93.691 kb on - strand, within MMJJ_RS00500 at 93.769 kb on + strand, within MMJJ_RS00500 at 93.772 kb on - strand, within MMJJ_RS00500 at 93.806 kb on + strand, within MMJJ_RS00500 at 94.015 kb on + strand at 94.015 kb on + strand at 94.051 kb on - strand at 94.051 kb on - strand at 94.051 kb on - strand at 94.052 kb on + strand at 94.053 kb on - strand at 94.053 kb on - strand at 94.054 kb on + strand at 94.070 kb on + strand, within MMJJ_RS00505 at 94.106 kb on + strand, within MMJJ_RS00505 at 94.118 kb on - strand, within MMJJ_RS00505 at 94.161 kb on - strand, within MMJJ_RS00505 at 94.184 kb on - strand, within MMJJ_RS00505 at 94.184 kb on - strand, within MMJJ_RS00505 at 94.184 kb on - strand, within MMJJ_RS00505 at 94.185 kb on + strand, within MMJJ_RS00505 at 94.186 kb on - strand, within MMJJ_RS00505 at 94.186 kb on - strand, within MMJJ_RS00505 at 94.209 kb on + strand, within MMJJ_RS00505 at 94.258 kb on - strand, within MMJJ_RS00505 at 94.258 kb on - strand, within MMJJ_RS00505 at 94.258 kb on - strand, within MMJJ_RS00505 at 94.259 kb on + strand, within MMJJ_RS00505 at 94.259 kb on + strand, within MMJJ_RS00505 at 94.300 kb on + strand, within MMJJ_RS00505 at 94.301 kb on - strand, within MMJJ_RS00505 at 94.313 kb on - strand, within MMJJ_RS00505 at 94.404 kb on - strand at 94.404 kb on - strand, within MMJJ_RS00505 at 94.404 kb on - strand, within MMJJ_RS00505 at 94.405 kb on + strand, within MMJJ_RS00505 at 94.415 kb on - strand, within MMJJ_RS00505 at 94.415 kb on - strand, within MMJJ_RS00505 at 94.416 kb on + strand, within MMJJ_RS00505 at 94.416 kb on + strand, within MMJJ_RS00505 at 94.416 kb on + strand, within MMJJ_RS00505 at 94.416 kb on + strand, within MMJJ_RS00505 at 94.492 kb on + strand, within MMJJ_RS00505 at 94.492 kb on + strand, within MMJJ_RS00505 at 94.509 kb on - strand, within MMJJ_RS00505 at 94.510 kb on + strand, within MMJJ_RS00505 at 94.514 kb on + strand, within MMJJ_RS00505 at 94.564 kb on + strand at 94.573 kb on - strand at 94.573 kb on - strand at 94.573 kb on - strand at 94.574 kb on + strand at 94.574 kb on + strand at 94.574 kb on + strand at 94.591 kb on - strand at 94.597 kb on - strand at 94.598 kb on + strand at 94.608 kb on - strand at 94.624 kb on + strand at 94.632 kb on - strand at 94.632 kb on - strand at 94.632 kb on - strand at 94.632 kb on - strand at 94.632 kb on - strand at 94.633 kb on + strand at 94.633 kb on + strand at 94.633 kb on + strand at 94.633 kb on + strand at 94.633 kb on + strand at 94.633 kb on + strand at 94.633 kb on + strand at 94.634 kb on - strand at 94.634 kb on - strand at 94.634 kb on - strand at 94.634 kb on - strand at 94.634 kb on - strand at 94.634 kb on - strand at 94.635 kb on + strand at 94.635 kb on + strand at 94.635 kb on + strand at 94.635 kb on + strand at 94.635 kb on + strand at 94.635 kb on + strand at 94.635 kb on + strand at 94.635 kb on + strand at 94.635 kb on + strand at 94.635 kb on + strand at 94.635 kb on + strand at 94.635 kb on + strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.636 kb on - strand at 94.637 kb on + strand at 94.637 kb on + strand at 94.637 kb on + strand at 94.637 kb on + strand at 94.637 kb on + strand at 94.637 kb on + strand at 94.637 kb on + strand at 94.637 kb on + strand at 94.637 kb on + strand at 94.637 kb on + strand at 94.637 kb on + strand at 94.637 kb on + strand at 94.639 kb on + strand at 94.650 kb on - strand at 94.650 kb on - strand at 94.651 kb on + strand at 94.657 kb on - strand at 94.657 kb on - strand at 94.657 kb on - strand at 94.657 kb on - strand at 94.658 kb on + strand at 94.660 kb on + strand at 94.663 kb on - strand at 94.663 kb on - strand at 94.663 kb on - strand at 94.663 kb on - strand at 94.664 kb on + strand at 94.664 kb on + strand at 94.664 kb on + strand at 94.667 kb on - strand at 94.671 kb on - strand at 94.672 kb on + strand at 94.675 kb on - strand at 94.683 kb on + strand at 94.684 kb on - strand at 94.685 kb on + strand at 94.691 kb on - strand at 94.704 kb on - strand at 94.704 kb on - strand at 94.708 kb on - strand at 94.708 kb on - strand at 94.775 kb on + strand at 94.797 kb on - strand at 94.799 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas-formate glass attached remove 93,270 - -2.0 93,381 - -4.0 93,381 - -0.4 93,382 + -0.5 93,382 + -4.0 93,382 + +1.6 93,382 + +0.4 93,386 + +0.7 93,398 - +0.1 93,398 - +0.1 93,399 + +0.3 93,399 + -0.1 93,399 + -0.7 93,406 + -0.2 93,411 - +1.0 93,412 + -2.9 93,413 - +0.2 93,413 - -0.7 93,413 - -1.8 93,413 - -0.7 93,414 + -0.7 93,443 + -0.5 93,455 + -4.3 93,455 + -0.2 93,459 - -0.2 93,460 + -1.7 93,460 + -1.2 93,486 - +0.4 93,486 - -0.8 93,486 - -2.1 93,486 - +0.2 93,601 - MMJJ_RS00500 0.29 +0.4 93,632 + MMJJ_RS00500 0.34 -0.7 93,653 + MMJJ_RS00500 0.38 -1.8 93,656 + MMJJ_RS00500 0.39 -0.3 93,691 - MMJJ_RS00500 0.45 -1.4 93,691 - MMJJ_RS00500 0.45 +0.1 93,769 + MMJJ_RS00500 0.59 -0.5 93,772 - MMJJ_RS00500 0.60 -0.3 93,806 + MMJJ_RS00500 0.66 -1.1 94,015 + -1.3 94,015 + -0.1 94,051 - +2.3 94,051 - -0.9 94,051 - -0.1 94,052 + -0.4 94,053 - -0.9 94,053 - +0.0 94,054 + -0.8 94,070 + MMJJ_RS00505 0.12 -0.2 94,106 + MMJJ_RS00505 0.18 +0.5 94,118 - MMJJ_RS00505 0.20 -0.4 94,161 - MMJJ_RS00505 0.28 +0.6 94,184 - MMJJ_RS00505 0.32 +0.6 94,184 - MMJJ_RS00505 0.32 +0.5 94,184 - MMJJ_RS00505 0.32 +0.5 94,185 + MMJJ_RS00505 0.32 +0.2 94,186 - MMJJ_RS00505 0.32 +0.3 94,186 - MMJJ_RS00505 0.32 -0.0 94,209 + MMJJ_RS00505 0.36 -1.1 94,258 - MMJJ_RS00505 0.44 -1.9 94,258 - MMJJ_RS00505 0.44 +0.0 94,258 - MMJJ_RS00505 0.44 -4.0 94,259 + MMJJ_RS00505 0.45 +0.4 94,259 + MMJJ_RS00505 0.45 -1.0 94,300 + MMJJ_RS00505 0.52 +0.5 94,301 - MMJJ_RS00505 0.52 +0.2 94,313 - MMJJ_RS00505 0.54 -0.3 94,404 - +0.6 94,404 - MMJJ_RS00505 0.70 -0.0 94,404 - MMJJ_RS00505 0.70 -1.7 94,405 + MMJJ_RS00505 0.70 -3.6 94,415 - MMJJ_RS00505 0.72 +2.1 94,415 - MMJJ_RS00505 0.72 +0.1 94,416 + MMJJ_RS00505 0.72 +0.9 94,416 + MMJJ_RS00505 0.72 +0.0 94,416 + MMJJ_RS00505 0.72 +1.4 94,416 + MMJJ_RS00505 0.72 +0.3 94,492 + MMJJ_RS00505 0.85 +0.1 94,492 + MMJJ_RS00505 0.85 -0.2 94,509 - MMJJ_RS00505 0.88 -0.3 94,510 + MMJJ_RS00505 0.88 +0.3 94,514 + MMJJ_RS00505 0.89 +0.8 94,564 + -0.3 94,573 - -0.4 94,573 - +0.8 94,573 - -0.0 94,574 + -1.2 94,574 + +0.5 94,574 + -2.9 94,591 - +0.6 94,597 - -0.4 94,598 + +0.5 94,608 - -0.1 94,624 + -0.6 94,632 - +0.5 94,632 - +0.8 94,632 - +1.5 94,632 - +0.6 94,632 - -0.7 94,633 + -0.1 94,633 + -1.0 94,633 + -0.8 94,633 + +0.1 94,633 + +0.3 94,633 + -0.2 94,633 + -1.3 94,634 - +0.4 94,634 - -4.5 94,634 - -0.7 94,634 - -0.5 94,634 - -0.4 94,634 - -0.3 94,635 + +0.1 94,635 + +0.5 94,635 + +0.8 94,635 + +1.1 94,635 + -2.3 94,635 + -0.8 94,635 + -0.9 94,635 + +2.0 94,635 + -0.5 94,635 + +0.3 94,635 + -0.3 94,635 + +0.5 94,636 - +0.8 94,636 - -0.8 94,636 - +1.3 94,636 - +0.2 94,636 - -1.7 94,636 - -0.7 94,636 - -2.2 94,636 - -0.9 94,636 - -0.6 94,636 - +0.7 94,636 - -0.3 94,636 - -0.1 94,636 - +0.9 94,636 - -0.5 94,636 - +1.1 94,636 - -0.2 94,637 + +0.3 94,637 + -0.5 94,637 + +0.3 94,637 + +0.4 94,637 + +1.0 94,637 + +0.6 94,637 + -0.1 94,637 + -1.6 94,637 + +0.4 94,637 + +1.0 94,637 + -3.1 94,637 + -2.1 94,639 + -2.8 94,650 - -0.1 94,650 - -0.3 94,651 + +0.9 94,657 - +1.0 94,657 - +1.9 94,657 - +0.2 94,657 - +1.0 94,658 + +0.6 94,660 + -0.3 94,663 - +0.8 94,663 - +0.7 94,663 - -0.8 94,663 - +0.2 94,664 + -2.8 94,664 + +0.4 94,664 + -2.0 94,667 - +0.4 94,671 - -3.4 94,672 + -3.5 94,675 - -2.3 94,683 + -0.5 94,684 - -0.1 94,685 + -0.0 94,691 - +0.4 94,704 - -0.3 94,704 - -0.3 94,708 - +0.9 94,708 - +0.5 94,775 + -0.6 94,797 - -0.7 94,799 - +0.2
Or see this region's nucleotide sequence