Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3224

Experiment: 7H9 with Dodecyl Sulfate 0.02 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3222c and Rv3223c overlap by 4 nucleotidesRv3223c and Rv3224 are separated by 299 nucleotidesRv3224 and Rv3224A overlap by 65 nucleotidesRv3224A and Rv3224B overlap by 23 nucleotidesRv3224B and Rv3225c overlap by 4 nucleotides Rv3222c: Rv3222c - Conserved hypothetical protein, at 3,598,353 to 3,598,904 Rv3222c Rv3223c: Rv3223c - Alternative RNA polymerase sigma-E factor (sigma-24) SigH (RPOE), at 3,598,901 to 3,599,551 Rv3223c Rv3224: Rv3224 - Possible iron-regulated short-chain dehydrogenase/reductase, at 3,599,851 to 3,600,699 Rv3224 Rv3224A: Rv3224A - Conserved hypothetical protein, at 3,600,635 to 3,600,823 Rv3224A Rv3224B: Rv3224B - Conserved hypothetical protein, at 3,600,801 to 3,601,019 Rv3224B Rv3225c: Rv3225c - GCN5-related N-acetyltransferase, phosphorylase, at 3,601,016 to 3,602,440 Rv3225c Position (kb) 3599 3600 3601Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3599.082 kb on + strand, within Rv3223cat 3599.083 kb on - strand, within Rv3223cat 3599.153 kb on + strand, within Rv3223cat 3599.271 kb on + strand, within Rv3223cat 3599.283 kb on + strand, within Rv3223cat 3599.284 kb on - strand, within Rv3223cat 3599.284 kb on - strand, within Rv3223cat 3599.356 kb on - strand, within Rv3223cat 3599.356 kb on - strand, within Rv3223cat 3599.431 kb on - strand, within Rv3223cat 3599.431 kb on - strand, within Rv3223cat 3599.559 kb on - strandat 3599.559 kb on - strandat 3599.690 kb on + strandat 3599.690 kb on + strandat 3599.691 kb on - strandat 3599.691 kb on - strandat 3599.691 kb on - strandat 3599.778 kb on + strandat 3600.123 kb on + strand, within Rv3224at 3600.124 kb on - strand, within Rv3224at 3600.124 kb on - strand, within Rv3224at 3600.124 kb on - strand, within Rv3224at 3600.155 kb on + strand, within Rv3224at 3600.155 kb on + strand, within Rv3224at 3600.156 kb on - strand, within Rv3224at 3600.223 kb on + strand, within Rv3224at 3600.223 kb on + strand, within Rv3224at 3600.224 kb on - strand, within Rv3224at 3600.224 kb on - strand, within Rv3224at 3600.224 kb on - strand, within Rv3224at 3600.224 kb on - strand, within Rv3224at 3600.230 kb on + strand, within Rv3224at 3600.231 kb on - strand, within Rv3224at 3600.252 kb on + strand, within Rv3224at 3600.252 kb on + strand, within Rv3224at 3600.253 kb on - strand, within Rv3224at 3600.253 kb on - strand, within Rv3224at 3600.334 kb on + strand, within Rv3224at 3600.334 kb on + strand, within Rv3224at 3600.335 kb on - strand, within Rv3224at 3600.335 kb on - strand, within Rv3224at 3600.335 kb on - strand, within Rv3224at 3600.335 kb on - strand, within Rv3224at 3600.349 kb on + strand, within Rv3224at 3600.349 kb on + strand, within Rv3224at 3600.349 kb on + strand, within Rv3224at 3600.507 kb on - strand, within Rv3224at 3600.507 kb on - strand, within Rv3224at 3600.508 kb on + strand, within Rv3224at 3600.508 kb on + strand, within Rv3224at 3600.508 kb on + strand, within Rv3224at 3600.509 kb on - strand, within Rv3224at 3600.523 kb on + strand, within Rv3224at 3600.523 kb on + strand, within Rv3224at 3600.523 kb on + strand, within Rv3224at 3600.524 kb on - strand, within Rv3224at 3600.554 kb on - strand, within Rv3224at 3600.619 kb on + strandat 3600.822 kb on - strandat 3601.017 kb on + strandat 3601.030 kb on + strandat 3601.111 kb on - strandat 3601.114 kb on - strandat 3601.379 kb on + strand, within Rv3225cat 3601.451 kb on + strand, within Rv3225cat 3601.685 kb on + strand, within Rv3225c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Dodecyl Sulfate 0.02 vol%
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3,599,082 + Rv3223c 0.28 +0.8
3,599,083 - Rv3223c 0.28 -0.8
3,599,153 + Rv3223c 0.39 -0.3
3,599,271 + Rv3223c 0.57 +0.8
3,599,283 + Rv3223c 0.59 +1.8
3,599,284 - Rv3223c 0.59 +0.3
3,599,284 - Rv3223c 0.59 -0.0
3,599,356 - Rv3223c 0.70 -1.5
3,599,356 - Rv3223c 0.70 +0.0
3,599,431 - Rv3223c 0.81 +1.2
3,599,431 - Rv3223c 0.81 -0.0
3,599,559 - +0.8
3,599,559 - -0.6
3,599,690 + +0.1
3,599,690 + -0.7
3,599,691 - -0.3
3,599,691 - -1.0
3,599,691 - +0.4
3,599,778 + -0.5
3,600,123 + Rv3224 0.32 +1.8
3,600,124 - Rv3224 0.32 -1.1
3,600,124 - Rv3224 0.32 +1.2
3,600,124 - Rv3224 0.32 -1.5
3,600,155 + Rv3224 0.36 -0.5
3,600,155 + Rv3224 0.36 -1.0
3,600,156 - Rv3224 0.36 +0.4
3,600,223 + Rv3224 0.44 -1.9
3,600,223 + Rv3224 0.44 +0.3
3,600,224 - Rv3224 0.44 -0.4
3,600,224 - Rv3224 0.44 -0.5
3,600,224 - Rv3224 0.44 -1.0
3,600,224 - Rv3224 0.44 +1.0
3,600,230 + Rv3224 0.45 -1.0
3,600,231 - Rv3224 0.45 -0.8
3,600,252 + Rv3224 0.47 -0.3
3,600,252 + Rv3224 0.47 -0.6
3,600,253 - Rv3224 0.47 +0.4
3,600,253 - Rv3224 0.47 -1.2
3,600,334 + Rv3224 0.57 -0.5
3,600,334 + Rv3224 0.57 -0.7
3,600,335 - Rv3224 0.57 +1.4
3,600,335 - Rv3224 0.57 -0.2
3,600,335 - Rv3224 0.57 +3.4
3,600,335 - Rv3224 0.57 -0.4
3,600,349 + Rv3224 0.59 -0.5
3,600,349 + Rv3224 0.59 -0.3
3,600,349 + Rv3224 0.59 -0.4
3,600,507 - Rv3224 0.77 -1.0
3,600,507 - Rv3224 0.77 -0.0
3,600,508 + Rv3224 0.77 -1.0
3,600,508 + Rv3224 0.77 -1.3
3,600,508 + Rv3224 0.77 +0.5
3,600,509 - Rv3224 0.78 -0.2
3,600,523 + Rv3224 0.79 -0.7
3,600,523 + Rv3224 0.79 -0.8
3,600,523 + Rv3224 0.79 -1.8
3,600,524 - Rv3224 0.79 -0.2
3,600,554 - Rv3224 0.83 -0.3
3,600,619 + +0.4
3,600,822 - -0.5
3,601,017 + +0.6
3,601,030 + -0.5
3,601,111 - -0.4
3,601,114 - -1.0
3,601,379 + Rv3225c 0.25 +1.5
3,601,451 + Rv3225c 0.31 +0.2
3,601,685 + Rv3225c 0.47 +0.1

Or see this region's nucleotide sequence