Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0408

Experiment: 7H9 with Hydrogen peroxide 3.97 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0407 and Rv0408 overlap by 8 nucleotidesRv0408 and Rv0409 overlap by 8 nucleotides Rv0407: Rv0407 - F420-dependent glucose-6-phosphate dehydrogenase Fgd1, at 490,783 to 491,793 Rv0407 Rv0408: Rv0408 - Probable phosphate acetyltransferase Pta (phosphotransacetylase), at 491,786 to 493,858 Rv0408 Rv0409: Rv0409 - Probable acetate kinase AckA (acetokinase), at 493,851 to 495,008 Rv0409 Position (kb) 491 492 493 494Strain fitness (log2 ratio) -2 -1 0 1 2at 490.799 kb on + strandat 490.804 kb on + strandat 490.804 kb on + strandat 490.805 kb on - strandat 490.805 kb on - strandat 491.196 kb on - strand, within Rv0407at 491.245 kb on + strand, within Rv0407at 491.300 kb on - strand, within Rv0407at 491.390 kb on - strand, within Rv0407at 491.486 kb on - strand, within Rv0407at 491.673 kb on - strand, within Rv0407at 491.680 kb on + strand, within Rv0407at 491.681 kb on - strand, within Rv0407at 491.681 kb on - strand, within Rv0407at 491.982 kb on - strandat 492.359 kb on + strand, within Rv0408at 492.360 kb on - strand, within Rv0408at 492.435 kb on + strand, within Rv0408at 492.869 kb on - strand, within Rv0408at 492.888 kb on - strand, within Rv0408at 493.356 kb on + strand, within Rv0408at 493.357 kb on - strand, within Rv0408at 493.364 kb on + strand, within Rv0408at 493.700 kb on + strandat 493.806 kb on + strandat 493.807 kb on - strandat 494.287 kb on - strand, within Rv0409at 494.358 kb on + strand, within Rv0409at 494.359 kb on - strand, within Rv0409at 494.359 kb on - strand, within Rv0409at 494.557 kb on - strand, within Rv0409at 494.666 kb on - strand, within Rv0409at 494.745 kb on + strand, within Rv0409at 494.852 kb on + strand, within Rv0409

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hydrogen peroxide 3.97 mM
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490,799 + +0.2
490,804 + +0.4
490,804 + -2.3
490,805 - -1.5
490,805 - +2.2
491,196 - Rv0407 0.41 -0.3
491,245 + Rv0407 0.46 -1.5
491,300 - Rv0407 0.51 -1.3
491,390 - Rv0407 0.60 -1.3
491,486 - Rv0407 0.70 +0.4
491,673 - Rv0407 0.88 -0.5
491,680 + Rv0407 0.89 +0.1
491,681 - Rv0407 0.89 -1.8
491,681 - Rv0407 0.89 -0.2
491,982 - -2.5
492,359 + Rv0408 0.28 -0.1
492,360 - Rv0408 0.28 +1.2
492,435 + Rv0408 0.31 -0.1
492,869 - Rv0408 0.52 -0.1
492,888 - Rv0408 0.53 +0.4
493,356 + Rv0408 0.76 -0.4
493,357 - Rv0408 0.76 +0.6
493,364 + Rv0408 0.76 +1.5
493,700 + -0.2
493,806 + -0.4
493,807 - +0.4
494,287 - Rv0409 0.38 -0.6
494,358 + Rv0409 0.44 -0.4
494,359 - Rv0409 0.44 +1.4
494,359 - Rv0409 0.44 -0.7
494,557 - Rv0409 0.61 -0.8
494,666 - Rv0409 0.70 -0.4
494,745 + Rv0409 0.77 -1.2
494,852 + Rv0409 0.86 +0.7

Or see this region's nucleotide sequence