Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0176

Experiment: 7H9 with Menadione 0.005 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0174 and Rv0175 are separated by 35 nucleotidesRv0175 and Rv0176 overlap by 4 nucleotidesRv0176 and Rv0177 overlap by 4 nucleotidesRv0177 and Rv0178 overlap by 34 nucleotides Rv0174: Rv0174 - Mce-family protein Mce1F, at 205,231 to 206,778 Rv0174 Rv0175: Rv0175 - Probable conserved Mce associated membrane protein, at 206,814 to 207,455 Rv0175 Rv0176: Rv0176 - Probable conserved Mce associated transmembrane protein, at 207,452 to 208,420 Rv0176 Rv0177: Rv0177 - Probable conserved Mce associated protein, at 208,417 to 208,971 Rv0177 Rv0178: Rv0178 - Probable conserved Mce associated membrane protein, at 208,938 to 209,672 Rv0178 Position (kb) 207 208 209Strain fitness (log2 ratio) -2 -1 0 1 2 3at 206.623 kb on - strand, within Rv0174at 206.623 kb on - strand, within Rv0174at 206.636 kb on - strandat 206.711 kb on - strandat 206.711 kb on - strandat 206.795 kb on + strandat 206.808 kb on - strandat 207.420 kb on + strandat 207.422 kb on + strandat 207.423 kb on - strandat 207.423 kb on - strandat 208.010 kb on + strand, within Rv0176at 208.010 kb on + strand, within Rv0176at 208.092 kb on - strand, within Rv0176at 208.152 kb on - strand, within Rv0176at 208.208 kb on - strand, within Rv0176at 208.295 kb on - strand, within Rv0176at 208.295 kb on - strand, within Rv0176at 208.314 kb on - strand, within Rv0176at 208.315 kb on + strand, within Rv0176at 208.316 kb on - strand, within Rv0176at 208.726 kb on + strand, within Rv0177at 208.827 kb on + strand, within Rv0177at 208.828 kb on - strand, within Rv0177at 209.247 kb on + strand, within Rv0178

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Menadione 0.005 mM
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206,623 - Rv0174 0.90 -1.4
206,623 - Rv0174 0.90 +2.8
206,636 - -0.7
206,711 - +1.2
206,711 - -0.7
206,795 + -0.1
206,808 - -0.4
207,420 + -0.4
207,422 + -0.6
207,423 - -0.3
207,423 - -0.2
208,010 + Rv0176 0.58 -1.0
208,010 + Rv0176 0.58 -2.2
208,092 - Rv0176 0.66 -0.7
208,152 - Rv0176 0.72 -1.0
208,208 - Rv0176 0.78 -0.3
208,295 - Rv0176 0.87 -1.3
208,295 - Rv0176 0.87 -1.3
208,314 - Rv0176 0.89 -1.3
208,315 + Rv0176 0.89 +0.8
208,316 - Rv0176 0.89 -0.9
208,726 + Rv0177 0.56 -2.3
208,827 + Rv0177 0.74 -0.9
208,828 - Rv0177 0.74 +1.3
209,247 + Rv0178 0.42 -1.1

Or see this region's nucleotide sequence