Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3534c

Experiment: 7H9 with Menadione 0.005 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3533c and Rv3534c are separated by 98 nucleotidesRv3534c and Rv3535c overlap by 4 nucleotidesRv3535c and Rv3536c are separated by 10 nucleotides Rv3533c: Rv3533c - PPE family protein PPE62, at 3,970,705 to 3,972,453 Rv3533c Rv3534c: Rv3534c - Probable 4-hydroxy-2-oxovalerate aldolase (HOA), at 3,972,552 to 3,973,592 Rv3534c Rv3535c: Rv3535c - Probable acetaldehyde dehydrogenase (acetaldehyde dehydrogenase [acetylating]), at 3,973,589 to 3,974,500 Rv3535c Rv3536c: Rv3536c - Probable hydratase, at 3,974,511 to 3,975,296 Rv3536c Position (kb) 3972 3973 3974Strain fitness (log2 ratio) -2 -1 0 1at 3971.994 kb on - strand, within Rv3533cat 3971.994 kb on - strand, within Rv3533cat 3972.035 kb on + strand, within Rv3533cat 3972.036 kb on - strand, within Rv3533cat 3972.240 kb on - strand, within Rv3533cat 3972.240 kb on - strand, within Rv3533cat 3972.240 kb on - strand, within Rv3533cat 3972.420 kb on - strandat 3972.423 kb on + strandat 3972.423 kb on + strandat 3972.424 kb on - strandat 3972.446 kb on + strandat 3972.686 kb on - strand, within Rv3534cat 3972.728 kb on - strand, within Rv3534cat 3972.740 kb on - strand, within Rv3534cat 3973.100 kb on - strand, within Rv3534cat 3973.100 kb on - strand, within Rv3534cat 3973.414 kb on + strand, within Rv3534cat 3973.414 kb on + strand, within Rv3534cat 3973.414 kb on + strand, within Rv3534cat 3973.415 kb on - strand, within Rv3534cat 3973.415 kb on - strand, within Rv3534cat 3973.583 kb on + strandat 3973.604 kb on + strandat 3973.605 kb on - strandat 3973.803 kb on + strand, within Rv3535cat 3973.804 kb on - strand, within Rv3535cat 3973.804 kb on - strand, within Rv3535cat 3974.238 kb on + strand, within Rv3535cat 3974.265 kb on + strand, within Rv3535cat 3974.300 kb on - strand, within Rv3535cat 3974.300 kb on - strand, within Rv3535cat 3974.459 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Menadione 0.005 mM
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3,971,994 - Rv3533c 0.74 +0.2
3,971,994 - Rv3533c 0.74 -0.6
3,972,035 + Rv3533c 0.76 +0.7
3,972,036 - Rv3533c 0.76 +0.9
3,972,240 - Rv3533c 0.88 +1.7
3,972,240 - Rv3533c 0.88 -2.0
3,972,240 - Rv3533c 0.88 -0.5
3,972,420 - -0.2
3,972,423 + +0.3
3,972,423 + -0.3
3,972,424 - +1.7
3,972,446 + +0.2
3,972,686 - Rv3534c 0.13 -0.6
3,972,728 - Rv3534c 0.17 -2.3
3,972,740 - Rv3534c 0.18 +0.1
3,973,100 - Rv3534c 0.53 -1.3
3,973,100 - Rv3534c 0.53 +0.1
3,973,414 + Rv3534c 0.83 -0.6
3,973,414 + Rv3534c 0.83 -1.4
3,973,414 + Rv3534c 0.83 -1.1
3,973,415 - Rv3534c 0.83 -1.5
3,973,415 - Rv3534c 0.83 +0.9
3,973,583 + -0.6
3,973,604 + -0.5
3,973,605 - -0.5
3,973,803 + Rv3535c 0.23 +0.1
3,973,804 - Rv3535c 0.24 -0.2
3,973,804 - Rv3535c 0.24 -0.1
3,974,238 + Rv3535c 0.71 -0.3
3,974,265 + Rv3535c 0.74 -0.6
3,974,300 - Rv3535c 0.78 -0.2
3,974,300 - Rv3535c 0.78 -1.2
3,974,459 - -0.9

Or see this region's nucleotide sequence