Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2883c

Experiment: 7H9 with Menadione 0.005 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2881c and Rv2882c are separated by 22 nucleotidesRv2882c and Rv2883c are separated by 171 nucleotidesRv2883c and Rv2884 are separated by 234 nucleotides Rv2881c: Rv2881c - Probable integral membrane phosphatidate cytidylyltransferase CdsA (CDP-diglyceride synthetase) (CDP-diglyceride pyrophosphorylase) (CDP-diacylglycerol synthase) (CDS) (CTP:phosphatidate cytidylyltransferase) (CDP-DAG synthase) (CDP-DG synthetase), at 3,190,701 to 3,191,621 Rv2881c Rv2882c: Rv2882c - Ribosome recycling factor Frr (ribosome releasing factor) (RRF), at 3,191,644 to 3,192,201 Rv2882c Rv2883c: Rv2883c - Probable uridylate kinase PyrH (UK) (uridine monophosphate kinase) (UMP kinase), at 3,192,373 to 3,193,158 Rv2883c Rv2884: Rv2884 - Probable transcriptional regulatory protein, at 3,193,393 to 3,194,151 Rv2884 Position (kb) 3192 3193 3194Strain fitness (log2 ratio) -2 -1 0 1at 3192.375 kb on - strandat 3193.254 kb on - strandat 3193.254 kb on - strandat 3193.339 kb on + strandat 3193.461 kb on - strandat 3193.467 kb on + strandat 3193.468 kb on - strandat 3193.645 kb on - strand, within Rv2884at 3193.849 kb on + strand, within Rv2884at 3193.999 kb on - strand, within Rv2884at 3194.015 kb on + strand, within Rv2884at 3194.015 kb on + strand, within Rv2884at 3194.083 kb on + strandat 3194.084 kb on - strandat 3194.084 kb on - strandat 3194.086 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Menadione 0.005 mM
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3,192,375 - +0.4
3,193,254 - -2.3
3,193,254 - -0.6
3,193,339 + -0.6
3,193,461 - -0.2
3,193,467 + +0.1
3,193,468 - -0.1
3,193,645 - Rv2884 0.33 +1.6
3,193,849 + Rv2884 0.60 -1.0
3,193,999 - Rv2884 0.80 -0.6
3,194,015 + Rv2884 0.82 -0.5
3,194,015 + Rv2884 0.82 -0.6
3,194,083 + -0.8
3,194,084 - -1.0
3,194,084 - +0.6
3,194,086 - +0.5

Or see this region's nucleotide sequence