Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2527

Experiment: 7H9 with Menadione 0.005 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2525c and Rv2526 are separated by 516 nucleotidesRv2526 and Rv2527 overlap by 4 nucleotidesRv2527 and Rv2528c are separated by 34 nucleotides Rv2525c: Rv2525c - Conserved hypothetical protein. Secreted; predicted to be a substrate of the twin arginine translocation (tat) export system., at 2,849,852 to 2,850,574 Rv2525c Rv2526: Rv2526 - Possible antitoxin VapB17, at 2,851,091 to 2,851,318 Rv2526 Rv2527: Rv2527 - Possible toxin VapC17, at 2,851,315 to 2,851,716 Rv2527 Rv2528c: Rv2528c - Probable restriction system protein Mrr, at 2,851,751 to 2,852,671 Rv2528c Position (kb) 2851 2852Strain fitness (log2 ratio) -1 0 1at 2850.381 kb on + strand, within Rv2525cat 2850.444 kb on + strand, within Rv2525cat 2850.445 kb on - strand, within Rv2525cat 2850.445 kb on - strand, within Rv2525cat 2850.713 kb on + strandat 2850.714 kb on - strandat 2850.714 kb on - strandat 2850.717 kb on - strandat 2850.732 kb on - strandat 2850.801 kb on + strandat 2850.801 kb on + strandat 2850.802 kb on - strandat 2850.851 kb on + strandat 2850.913 kb on - strandat 2850.913 kb on - strandat 2851.082 kb on + strandat 2851.098 kb on + strandat 2851.436 kb on - strand, within Rv2527at 2851.436 kb on - strand, within Rv2527at 2851.436 kb on - strand, within Rv2527at 2851.460 kb on - strand, within Rv2527at 2851.490 kb on - strand, within Rv2527at 2851.551 kb on - strand, within Rv2527at 2851.595 kb on + strand, within Rv2527at 2851.655 kb on - strand, within Rv2527at 2852.017 kb on - strand, within Rv2528cat 2852.035 kb on - strand, within Rv2528cat 2852.506 kb on - strand, within Rv2528cat 2852.506 kb on - strand, within Rv2528cat 2852.625 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Menadione 0.005 mM
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2,850,381 + Rv2525c 0.73 -0.1
2,850,444 + Rv2525c 0.82 -0.2
2,850,445 - Rv2525c 0.82 +0.1
2,850,445 - Rv2525c 0.82 +0.1
2,850,713 + -0.6
2,850,714 - -0.1
2,850,714 - -0.3
2,850,717 - +0.4
2,850,732 - -1.6
2,850,801 + -0.8
2,850,801 + +0.1
2,850,802 - -0.0
2,850,851 + -0.6
2,850,913 - +0.7
2,850,913 - -0.5
2,851,082 + +0.1
2,851,098 + -0.6
2,851,436 - Rv2527 0.30 +1.1
2,851,436 - Rv2527 0.30 +1.3
2,851,436 - Rv2527 0.30 +0.6
2,851,460 - Rv2527 0.36 -0.6
2,851,490 - Rv2527 0.44 -0.1
2,851,551 - Rv2527 0.59 -0.1
2,851,595 + Rv2527 0.70 +0.7
2,851,655 - Rv2527 0.85 -1.0
2,852,017 - Rv2528c 0.29 +1.2
2,852,035 - Rv2528c 0.31 -0.2
2,852,506 - Rv2528c 0.82 -0.6
2,852,506 - Rv2528c 0.82 -0.0
2,852,625 + -0.3

Or see this region's nucleotide sequence