Experiment: 0.1X LB plate, Mixed culture; Micromonospora sp. SAI_152 1:1 starding OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00365 and MPMX20_00366 overlap by 1 nucleotides MPMX20_00366 and MPMX20_00367 are separated by 242 nucleotides
MPMX20_00365: MPMX20_00365 - D-beta-hydroxybutyrate dehydrogenase, at 382,802 to 383,587
_00365
MPMX20_00366: MPMX20_00366 - Acetyl esterase, at 383,587 to 384,672
_00366
MPMX20_00367: MPMX20_00367 - hypothetical protein, at 384,915 to 385,832
_00367
Position (kb) 383
384
385 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 382.604 kb on + strand at 382.618 kb on + strand at 382.618 kb on + strand at 382.626 kb on - strand at 382.626 kb on - strand at 382.663 kb on + strand at 382.664 kb on - strand at 382.676 kb on + strand at 382.724 kb on - strand at 382.726 kb on + strand at 382.726 kb on + strand at 382.726 kb on + strand at 382.729 kb on - strand at 382.734 kb on - strand at 382.739 kb on - strand at 382.772 kb on + strand at 382.831 kb on + strand at 382.845 kb on + strand at 382.853 kb on - strand at 382.855 kb on + strand at 382.863 kb on - strand at 382.874 kb on + strand at 382.900 kb on + strand, within MPMX20_00365 at 382.903 kb on - strand, within MPMX20_00365 at 382.939 kb on + strand, within MPMX20_00365 at 382.957 kb on + strand, within MPMX20_00365 at 382.960 kb on + strand, within MPMX20_00365 at 382.972 kb on - strand, within MPMX20_00365 at 382.989 kb on - strand, within MPMX20_00365 at 382.991 kb on - strand, within MPMX20_00365 at 382.991 kb on - strand, within MPMX20_00365 at 383.002 kb on + strand, within MPMX20_00365 at 383.002 kb on + strand, within MPMX20_00365 at 383.007 kb on + strand, within MPMX20_00365 at 383.010 kb on - strand, within MPMX20_00365 at 383.022 kb on + strand, within MPMX20_00365 at 383.022 kb on + strand, within MPMX20_00365 at 383.030 kb on - strand, within MPMX20_00365 at 383.041 kb on + strand, within MPMX20_00365 at 383.067 kb on + strand, within MPMX20_00365 at 383.096 kb on + strand, within MPMX20_00365 at 383.096 kb on + strand, within MPMX20_00365 at 383.096 kb on + strand, within MPMX20_00365 at 383.104 kb on - strand, within MPMX20_00365 at 383.104 kb on - strand, within MPMX20_00365 at 383.104 kb on - strand, within MPMX20_00365 at 383.147 kb on + strand, within MPMX20_00365 at 383.154 kb on - strand, within MPMX20_00365 at 383.185 kb on + strand, within MPMX20_00365 at 383.185 kb on + strand, within MPMX20_00365 at 383.187 kb on + strand, within MPMX20_00365 at 383.193 kb on - strand, within MPMX20_00365 at 383.262 kb on - strand, within MPMX20_00365 at 383.266 kb on + strand, within MPMX20_00365 at 383.266 kb on + strand, within MPMX20_00365 at 383.276 kb on + strand, within MPMX20_00365 at 383.330 kb on + strand, within MPMX20_00365 at 383.336 kb on - strand, within MPMX20_00365 at 383.338 kb on - strand, within MPMX20_00365 at 383.353 kb on - strand, within MPMX20_00365 at 383.358 kb on - strand, within MPMX20_00365 at 383.374 kb on + strand, within MPMX20_00365 at 383.399 kb on + strand, within MPMX20_00365 at 383.404 kb on - strand, within MPMX20_00365 at 383.406 kb on + strand, within MPMX20_00365 at 383.406 kb on + strand, within MPMX20_00365 at 383.406 kb on + strand, within MPMX20_00365 at 383.432 kb on - strand, within MPMX20_00365 at 383.442 kb on + strand, within MPMX20_00365 at 383.476 kb on + strand, within MPMX20_00365 at 383.497 kb on + strand, within MPMX20_00365 at 383.540 kb on - strand at 383.553 kb on + strand at 383.571 kb on + strand at 383.639 kb on - strand at 383.666 kb on + strand at 383.690 kb on + strand at 383.711 kb on + strand, within MPMX20_00366 at 383.757 kb on + strand, within MPMX20_00366 at 383.788 kb on - strand, within MPMX20_00366 at 383.802 kb on - strand, within MPMX20_00366 at 383.802 kb on - strand, within MPMX20_00366 at 383.813 kb on + strand, within MPMX20_00366 at 383.830 kb on + strand, within MPMX20_00366 at 383.885 kb on - strand, within MPMX20_00366 at 383.953 kb on - strand, within MPMX20_00366 at 383.983 kb on + strand, within MPMX20_00366 at 383.983 kb on + strand, within MPMX20_00366 at 384.064 kb on + strand, within MPMX20_00366 at 384.092 kb on + strand, within MPMX20_00366 at 384.175 kb on + strand, within MPMX20_00366 at 384.215 kb on + strand, within MPMX20_00366 at 384.215 kb on + strand, within MPMX20_00366 at 384.223 kb on - strand, within MPMX20_00366 at 384.223 kb on - strand, within MPMX20_00366 at 384.223 kb on - strand, within MPMX20_00366 at 384.223 kb on - strand, within MPMX20_00366 at 384.286 kb on + strand, within MPMX20_00366 at 384.310 kb on + strand, within MPMX20_00366 at 384.345 kb on + strand, within MPMX20_00366 at 384.408 kb on + strand, within MPMX20_00366 at 384.415 kb on + strand, within MPMX20_00366 at 384.472 kb on - strand, within MPMX20_00366 at 384.477 kb on - strand, within MPMX20_00366 at 384.479 kb on - strand, within MPMX20_00366 at 384.513 kb on + strand, within MPMX20_00366 at 384.513 kb on + strand, within MPMX20_00366 at 384.513 kb on + strand, within MPMX20_00366 at 384.521 kb on - strand, within MPMX20_00366 at 384.612 kb on + strand at 384.613 kb on + strand at 384.620 kb on - strand at 384.647 kb on + strand at 384.655 kb on - strand at 384.655 kb on - strand at 384.692 kb on + strand at 384.706 kb on + strand at 384.711 kb on + strand at 384.718 kb on + strand at 384.724 kb on - strand at 384.724 kb on - strand at 384.734 kb on + strand at 384.792 kb on - strand at 384.814 kb on + strand at 384.814 kb on + strand at 384.814 kb on + strand at 384.814 kb on + strand at 384.817 kb on - strand at 384.822 kb on - strand at 384.860 kb on - strand at 384.876 kb on + strand at 384.886 kb on + strand at 384.896 kb on + strand at 384.896 kb on + strand at 384.916 kb on + strand at 385.015 kb on - strand, within MPMX20_00367 at 385.017 kb on + strand, within MPMX20_00367 at 385.084 kb on + strand, within MPMX20_00367 at 385.084 kb on + strand, within MPMX20_00367 at 385.092 kb on - strand, within MPMX20_00367 at 385.101 kb on - strand, within MPMX20_00367 at 385.101 kb on - strand, within MPMX20_00367 at 385.175 kb on + strand, within MPMX20_00367 at 385.182 kb on + strand, within MPMX20_00367 at 385.185 kb on - strand, within MPMX20_00367 at 385.192 kb on + strand, within MPMX20_00367 at 385.192 kb on + strand, within MPMX20_00367 at 385.200 kb on - strand, within MPMX20_00367 at 385.200 kb on - strand, within MPMX20_00367 at 385.200 kb on - strand, within MPMX20_00367 at 385.204 kb on + strand, within MPMX20_00367 at 385.209 kb on + strand, within MPMX20_00367 at 385.210 kb on + strand, within MPMX20_00367 at 385.218 kb on - strand, within MPMX20_00367 at 385.220 kb on + strand, within MPMX20_00367 at 385.220 kb on + strand, within MPMX20_00367 at 385.223 kb on - strand, within MPMX20_00367 at 385.228 kb on - strand, within MPMX20_00367 at 385.228 kb on - strand, within MPMX20_00367 at 385.228 kb on - strand, within MPMX20_00367 at 385.228 kb on - strand, within MPMX20_00367 at 385.228 kb on - strand, within MPMX20_00367 at 385.228 kb on - strand, within MPMX20_00367 at 385.228 kb on - strand, within MPMX20_00367 at 385.228 kb on - strand, within MPMX20_00367 at 385.233 kb on - strand, within MPMX20_00367 at 385.257 kb on + strand, within MPMX20_00367 at 385.265 kb on - strand, within MPMX20_00367 at 385.265 kb on - strand, within MPMX20_00367 at 385.322 kb on + strand, within MPMX20_00367 at 385.361 kb on + strand, within MPMX20_00367 at 385.419 kb on - strand, within MPMX20_00367 at 385.445 kb on + strand, within MPMX20_00367 at 385.445 kb on + strand, within MPMX20_00367 at 385.448 kb on + strand, within MPMX20_00367 at 385.448 kb on + strand, within MPMX20_00367 at 385.453 kb on - strand, within MPMX20_00367 at 385.456 kb on - strand, within MPMX20_00367 at 385.461 kb on - strand, within MPMX20_00367 at 385.461 kb on - strand, within MPMX20_00367 at 385.462 kb on - strand, within MPMX20_00367 at 385.470 kb on + strand, within MPMX20_00367 at 385.572 kb on + strand, within MPMX20_00367 at 385.575 kb on + strand, within MPMX20_00367 at 385.595 kb on + strand, within MPMX20_00367 at 385.608 kb on - strand, within MPMX20_00367 at 385.627 kb on + strand, within MPMX20_00367 at 385.635 kb on + strand, within MPMX20_00367 at 385.643 kb on - strand, within MPMX20_00367 at 385.654 kb on + strand, within MPMX20_00367 at 385.662 kb on - strand, within MPMX20_00367 at 385.662 kb on - strand, within MPMX20_00367
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Micromonospora sp. SAI_152 1:1 starding OD to Enterobacter_TBS_079_ML3; Collection-direct remove 382,604 + +0.3 382,618 + +0.8 382,618 + +1.6 382,626 - -0.3 382,626 - -0.4 382,663 + +0.3 382,664 - +1.3 382,676 + -0.9 382,724 - -2.1 382,726 + +0.2 382,726 + -0.0 382,726 + +0.3 382,729 - +0.4 382,734 - -0.5 382,739 - -0.7 382,772 + -0.8 382,831 + -1.3 382,845 + -0.7 382,853 - -0.5 382,855 + -0.7 382,863 - -2.7 382,874 + -4.0 382,900 + MPMX20_00365 0.12 +0.5 382,903 - MPMX20_00365 0.13 -0.5 382,939 + MPMX20_00365 0.17 +5.4 382,957 + MPMX20_00365 0.20 -2.1 382,960 + MPMX20_00365 0.20 -0.5 382,972 - MPMX20_00365 0.22 -2.2 382,989 - MPMX20_00365 0.24 -1.0 382,991 - MPMX20_00365 0.24 -1.0 382,991 - MPMX20_00365 0.24 -0.1 383,002 + MPMX20_00365 0.25 +0.1 383,002 + MPMX20_00365 0.25 +1.3 383,007 + MPMX20_00365 0.26 +0.3 383,010 - MPMX20_00365 0.26 -1.8 383,022 + MPMX20_00365 0.28 -3.1 383,022 + MPMX20_00365 0.28 -2.1 383,030 - MPMX20_00365 0.29 -0.5 383,041 + MPMX20_00365 0.30 +0.8 383,067 + MPMX20_00365 0.34 -0.1 383,096 + MPMX20_00365 0.37 -1.2 383,096 + MPMX20_00365 0.37 +0.2 383,096 + MPMX20_00365 0.37 +0.0 383,104 - MPMX20_00365 0.38 -1.2 383,104 - MPMX20_00365 0.38 +1.9 383,104 - MPMX20_00365 0.38 +0.1 383,147 + MPMX20_00365 0.44 +1.9 383,154 - MPMX20_00365 0.45 -0.6 383,185 + MPMX20_00365 0.49 -0.6 383,185 + MPMX20_00365 0.49 +1.6 383,187 + MPMX20_00365 0.49 +0.5 383,193 - MPMX20_00365 0.50 +1.9 383,262 - MPMX20_00365 0.59 -0.7 383,266 + MPMX20_00365 0.59 +2.9 383,266 + MPMX20_00365 0.59 -2.1 383,276 + MPMX20_00365 0.60 -1.5 383,330 + MPMX20_00365 0.67 -0.4 383,336 - MPMX20_00365 0.68 +0.3 383,338 - MPMX20_00365 0.68 -1.6 383,353 - MPMX20_00365 0.70 -1.8 383,358 - MPMX20_00365 0.71 +0.7 383,374 + MPMX20_00365 0.73 +0.3 383,399 + MPMX20_00365 0.76 +1.8 383,404 - MPMX20_00365 0.77 +0.6 383,406 + MPMX20_00365 0.77 +0.0 383,406 + MPMX20_00365 0.77 -1.1 383,406 + MPMX20_00365 0.77 +6.1 383,432 - MPMX20_00365 0.80 -1.3 383,442 + MPMX20_00365 0.81 -0.3 383,476 + MPMX20_00365 0.86 -1.2 383,497 + MPMX20_00365 0.88 -0.3 383,540 - +0.3 383,553 + -0.7 383,571 + +0.4 383,639 - +0.3 383,666 + -3.4 383,690 + +0.4 383,711 + MPMX20_00366 0.11 +1.8 383,757 + MPMX20_00366 0.16 -0.5 383,788 - MPMX20_00366 0.19 -1.4 383,802 - MPMX20_00366 0.20 +0.9 383,802 - MPMX20_00366 0.20 -0.8 383,813 + MPMX20_00366 0.21 +1.0 383,830 + MPMX20_00366 0.22 -1.1 383,885 - MPMX20_00366 0.27 -4.2 383,953 - MPMX20_00366 0.34 -1.1 383,983 + MPMX20_00366 0.36 +0.6 383,983 + MPMX20_00366 0.36 +1.3 384,064 + MPMX20_00366 0.44 -0.1 384,092 + MPMX20_00366 0.47 -0.3 384,175 + MPMX20_00366 0.54 -2.3 384,215 + MPMX20_00366 0.58 -1.9 384,215 + MPMX20_00366 0.58 +0.3 384,223 - MPMX20_00366 0.59 -0.7 384,223 - MPMX20_00366 0.59 -0.1 384,223 - MPMX20_00366 0.59 -1.5 384,223 - MPMX20_00366 0.59 +0.7 384,286 + MPMX20_00366 0.64 +0.3 384,310 + MPMX20_00366 0.67 -0.1 384,345 + MPMX20_00366 0.70 -0.3 384,408 + MPMX20_00366 0.76 -1.3 384,415 + MPMX20_00366 0.76 -0.7 384,472 - MPMX20_00366 0.81 -0.7 384,477 - MPMX20_00366 0.82 -0.9 384,479 - MPMX20_00366 0.82 -0.5 384,513 + MPMX20_00366 0.85 -0.6 384,513 + MPMX20_00366 0.85 -0.4 384,513 + MPMX20_00366 0.85 -1.5 384,521 - MPMX20_00366 0.86 -1.4 384,612 + -0.7 384,613 + -0.7 384,620 - -1.5 384,647 + -0.3 384,655 - -0.6 384,655 - +1.3 384,692 + +1.0 384,706 + +1.2 384,711 + +0.5 384,718 + -0.0 384,724 - +0.5 384,724 - +0.6 384,734 + -1.9 384,792 - -1.0 384,814 + -0.3 384,814 + +0.4 384,814 + -2.4 384,814 + -0.6 384,817 - +0.4 384,822 - +1.5 384,860 - +0.6 384,876 + +0.7 384,886 + -0.4 384,896 + +1.3 384,896 + +0.4 384,916 + -1.0 385,015 - MPMX20_00367 0.11 -0.5 385,017 + MPMX20_00367 0.11 +0.7 385,084 + MPMX20_00367 0.18 -0.4 385,084 + MPMX20_00367 0.18 +0.6 385,092 - MPMX20_00367 0.19 +0.9 385,101 - MPMX20_00367 0.20 +3.6 385,101 - MPMX20_00367 0.20 -0.7 385,175 + MPMX20_00367 0.28 -1.5 385,182 + MPMX20_00367 0.29 +0.4 385,185 - MPMX20_00367 0.29 -2.4 385,192 + MPMX20_00367 0.30 -1.2 385,192 + MPMX20_00367 0.30 -1.1 385,200 - MPMX20_00367 0.31 +0.3 385,200 - MPMX20_00367 0.31 -0.7 385,200 - MPMX20_00367 0.31 +0.4 385,204 + MPMX20_00367 0.31 +0.4 385,209 + MPMX20_00367 0.32 -0.5 385,210 + MPMX20_00367 0.32 +2.3 385,218 - MPMX20_00367 0.33 +0.3 385,220 + MPMX20_00367 0.33 +0.2 385,220 + MPMX20_00367 0.33 +0.3 385,223 - MPMX20_00367 0.34 +0.8 385,228 - MPMX20_00367 0.34 -0.5 385,228 - MPMX20_00367 0.34 +0.4 385,228 - MPMX20_00367 0.34 +0.2 385,228 - MPMX20_00367 0.34 +1.1 385,228 - MPMX20_00367 0.34 +0.3 385,228 - MPMX20_00367 0.34 +0.2 385,228 - MPMX20_00367 0.34 +1.7 385,228 - MPMX20_00367 0.34 +0.3 385,233 - MPMX20_00367 0.35 -2.7 385,257 + MPMX20_00367 0.37 -0.7 385,265 - MPMX20_00367 0.38 -0.6 385,265 - MPMX20_00367 0.38 -3.0 385,322 + MPMX20_00367 0.44 -0.6 385,361 + MPMX20_00367 0.49 -0.8 385,419 - MPMX20_00367 0.55 -0.7 385,445 + MPMX20_00367 0.58 -1.7 385,445 + MPMX20_00367 0.58 +0.8 385,448 + MPMX20_00367 0.58 +0.5 385,448 + MPMX20_00367 0.58 -0.5 385,453 - MPMX20_00367 0.59 +1.3 385,456 - MPMX20_00367 0.59 -0.4 385,461 - MPMX20_00367 0.59 +0.0 385,461 - MPMX20_00367 0.59 -0.3 385,462 - MPMX20_00367 0.60 -0.1 385,470 + MPMX20_00367 0.60 +1.3 385,572 + MPMX20_00367 0.72 +0.3 385,575 + MPMX20_00367 0.72 -2.4 385,595 + MPMX20_00367 0.74 -3.5 385,608 - MPMX20_00367 0.75 -0.5 385,627 + MPMX20_00367 0.78 +1.4 385,635 + MPMX20_00367 0.78 -1.3 385,643 - MPMX20_00367 0.79 -1.5 385,654 + MPMX20_00367 0.81 +1.5 385,662 - MPMX20_00367 0.81 +0.3 385,662 - MPMX20_00367 0.81 -0.6
Or see this region's nucleotide sequence